Association of three putative periodontal pathogens with chronic periodontitis in Brazilian subjects

Abstract

Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis.Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples.Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively).Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.

Subgingival microbial profile of women with breast cancer: a cross-sectional study

“…and Prevotella sp. It is important to note that the microbial profile of our study population was in agreement with previous studies that found higher levels of these bacterial species in the subgingival biofilm of subjects with periodontitis [26][27][28] and no differences were observed between our data and the data provided by the literature. However, it is important to highlight that our study population presented a clinical profile compatible with mild periodontitis, and we also found a complex microbiota in their subgingival biofilm.…”

Multiplex real‐time PCR detection and relative quantification of periodontal pathogens

“…Real‐time quantitative PCR (qPCR) has been used as an approach to rapidly quantify specific periodontal pathogens (Masunaga et al, 2010). In addition to real‐time qPCR, other DNA‐based techniques being used in assessing the oral bacteria include checkerboard hybridization (Dahlen et al, 2016; Goncalves et al, 2016; Vieira Colombo et al, 2016). This technique compared with a real‐time qPCR is more labor intensive and time‐consuming.…”

“…Another limitation of checkerboard hybridization is the detection limit that requires the presence of about 10 4 to 10 6 bacterial cells in most samples (Brito et al, 2007). However, with this approach, the presence of a larger microbial population can be analyzed simultaneously (Dahlen et al, 2016; Goncalves et al, 2016; Vieira Colombo et al, 2016). The benefits of a real‐time qPCR, on the other hand, are that it offers a sensitive means of detecting and quantifying small number of bacteria in clinical samples.…”

Support vector machine-based differentiation between aggressive and chronic periodontitis using microbial profiles

“…In addition, several species belonging to different complexes have been associated with periodontal health, in particular those from the genera Actinomyces , Streptococcus and Capnocytophaga . It is also important to note that there is moderate evidence in the literature to support the existence of newly identified periodontal pathogens or host‐compatible species but the role of these species as true pathogens or as markers for periodontal stability are yet to be established, especially by risk assessment and interventional studies. Therefore, the 40 bacterial species defined by Socransky et al .…”