Multiplex real‐time PCR detection and relative quantification of periodontal pathogens

Coffey, Joshua; Choudhry, Mydah; Shlossman, Marc; Makin, Inder Raj S.; Singh, Vipender

doi:10.1002/cre2.37

Cited by 18 publications

(16 citation statements)

References 35 publications

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“…Given that S. cristatus, among other species, has been associated with severe early childhood caries [27], even in the absence of Streptococcus mutans, its role as an important cariogenic species and potential caries risk biomarker should be further studied. Nevertheless, it must be emphasized that streptococci are extremely similar in their 16S rRNA gene sequence, particularly at the V3-V4 region analyzed in the current work, and therefore the suggested association of S. cristatus with caries development should be confirmed by species-specific methodology with higher discriminatory power, like qPCR with specific primers and probes [94].…”

Section: Bacterial Composition and Caries Developmentmentioning

confidence: 77%

Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay

et al. 2018

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Information on how the oral microbiome develops during early childhood and how external factors influence this ecological process is scarce. We used high-throughput sequencing to characterize bacterial composition in saliva samples collected at 3, 6, 12, 24 months and 7 years of age in 90 longitudinally followed children, for whom clinical, dietary and health data were collected. Bacterial composition patterns changed through time, starting with "early colonizers", including Streptococcus and Veillonella; other bacterial genera such as Neisseria settled after 1 or 2 years of age. Dental caries development was associated with diverging microbial composition through time. Streptococcus cristatus appeared to be associated with increased risk of developing tooth decay and its role as potential biomarker of the disease should be studied with species-specific probes. Infants born by C-section had initially skewed bacterial content compared with vaginally delivered infants, but this was recovered with age. Shorter breastfeeding habits and antibiotic treatment during the first 2 years of age were associated with a distinct bacterial composition at later age. The findings presented describe oral microbiota development as an ecological succession where altered colonization pattern during the first year of life may have long-term consequences for child´s oral and systemic health.

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Section: Bacterial Composition and Caries Developmentmentioning

confidence: 77%

Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay

et al. 2018

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“…salivarius , and S . moorei (Coffey, Mydah Choudhry, & Shlossman, ; Corless et al, ; Rolph et al, ; Srinivasan, Gertz, Shewmaker, et al, ). The sequences of primers and TaqMan probes are presented in Table .…”

Section: Methodsmentioning

confidence: 99%

Efficacy of chlorine dioxide mouthwash in reducing oral malodor: A 2‐week randomized, double‐blind, crossover study

Pham

Nguyen

2018

Clinical & Exp Dental Res

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The aim of this clinical trial was to assess the inhibitory effects of a mouthwash containing 0.1% ClO2 used for 2 weeks on oral malodor, periodontal and salivary parameters, tongue coating, and Gram‐negative and Gram‐positive bacteria in saliva. Thirty‐nine subjects with oral malodor were randomly assigned into two groups. In the first stage, one group was instructed to rinse with the experimental mouthwash (containing 0.1% ClO2), whereas the other group was instructed to rinse with the control mouthwash (sodium chloride 0.9%) for 2 weeks. After 4 weeks of washing out, in the second stage, each group then used the other mouthwash for 2 weeks. Oral malodor, periodontal status, tongue coating, salivary pH and flow rate, and the amounts of the salivary bacteria Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Solobacterium moorei, Streptococcus salivarius, Treponema denticola, and Tannerella forsythia were evaluated at baseline and after 2 weeks of mouthwash use. After 12 hr and after 2 weeks, organoleptic scores and the levels of H2S and CH3SH were significantly lower in the experimental group compared with those in the control group. After 2 weeks, the experimental mouthwash appeared significantly effective in reducing plaque index, tongue‐coating score, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in the whole saliva, compared with those at baseline. Mouthwash containing 0.1% ClO2 is effective in reducing oral malodor, dental plaque, tongue‐coating accumulation, and the amounts of F. nucleatum, S. moorei, T. denticola, and T. forsythia in saliva.

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“…Polymerase chain reaction (PCR) was used to detect bacterial DNA using pairs of primers specific to the five periodontal pathogens: Aa, Pg, Td, Tf and Pi. (18,19) The presence of bacterial DNA was confirmed using a universal pair of primers that bind to the 16S rDNA sequence. (18) Each reaction was performed at a final volume of 25µL, containing 1x PCR buffer (Invitrogen, Life Technologies, SP,BR), 0.2mM of dNTP (Pharmacia Biotech, Piscataway, NJ, USA), 0.02U/µL of Platinum Taq polymerase (Invitrogen, Life Technologies, SP, BR), 0.3µM of each primer, 10ng of DNA Template and 14.15µL of RNAse-free H 2 O.…”

Section: Microbiological Evaluationmentioning

confidence: 99%

Microbiological findings of the maternal periodontitis associated to low birthweight

Oliveira

Filho

Stöcker

et al. 2020

Einstein (São Paulo)

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Objective: To determine the association between the presence of periodontal pathogens and low birthweight. Methods: This observational and case-control study consisted of mothers of infants weighing <2,500g (Group A), and mothers of newborns weighing ≥2,500g (Group B), born at Hospital da Mulher in Feira de Santana (BA), Brazil. A semi-structured questionnaire covering demographic data, gestational history and aspects related to general and oral health was employed postpartum. Following a complete periodontal examination, biofilm samples were collected at six sites in the mouth. The participants were further categorized in terms of presence or absence of periodontitis. Differences between the groups were determined using Pearson's χ 2 test, odds ratio, and confidence intervals were obtained using the Mantel-Haenszel test. Results: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Prevotella intermedia were detected by polymerase chain reaction. A total of 303 postpartum women were evaluated, 224 (73.9%) in Group B. Statistically significant differences between the groups were found for age, body mass index and history of previous low birthweight babies. Group A had a higher frequency of periodontitis (33.34%) than Group B (16.22%). P. gingivalis and P. intermedia were detected more frequently among women with periodontitis (74.19% and 88.70%, respectively). Conclusion: In this population, there was no association between the presence of maternal periodontal pathogens and the occurrence of low birthweight infants.

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Multiplex real‐time PCR detection and relative quantification of periodontal pathogens

Cited by 18 publications

References 35 publications

Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay

Oral microbiome development during childhood: an ecological succession influenced by postnatal factors and associated with tooth decay

Efficacy of chlorine dioxide mouthwash in reducing oral malodor: A 2‐week randomized, double‐blind, crossover study

Microbiological findings of the maternal periodontitis associated to low birthweight

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