Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

Azevedo‐Alanis, Luciana Reis; Tolentino, Elen de Souza; Assis, Gérson Francisco de; Cestari, Tânia Mary; Lara, Vanessa Soares; Damante, José Humberto

doi:10.1590/1678-775720150139

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…There were marked cytoplasmic vacuolization, nuclear alteration, autolysis, and acinar cells atrophy, besides mononuclear cells infiltration, increase the interstitial spaces, and decrease the number of intralobular ducts. These findings, to a great extent, are mimic those observed with oxidative stress [9,10], irradiation [11,12], and age-related changes, which have reported in human sublingual glands [13], labial glands [14,15] and salivary glands in mice [16]. Each morphological alteration is a consequent event for molecular changes that affect many reactions.…”

Section: Discussionsupporting

confidence: 81%

Histological and Biochemical Evaluation of the Effect of Desloratadine Drug in Parotid Gland Tissues

.¹,

.²,

.³

et al. 2021

amj

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Background: The functional deterioration of salivary glands is a consequence of a wide range of factors and significantly interfered with life quality. Desloratadine (an antihistamine) is among the drugs listed to cause dry mouth; however, its effect on major salivary gland tissues has not been well studied. Objectives: To evaluate the effects of desloratadine treatment on parotid gland tissues, histological features, and their impact on serum oxidative and antioxidant markers. Materials and methods: Thirty rats were used in this study. They were divided into three groups (each containing ten rats). Group A: control rats. Group B and C have received desloratadine at dose 0.142 and 0.245 mg/kg of body weight respectively for three weeks. After three weeks, serum levels of sialic acid, malondialdehyde, catalase, lactate dehydrogenase, superoxide dismutase, creatin kinase, and glutathione were estimated for three groups. Then, animals were sacrificed and five µm formalin-fixed paraffin-embedded tissue sections were prepared routinely from parotid glands for histological evaluation under light microscope. Results: The histological evaluation of salivary gland tissues in both treated-groups was revealed a remarkable cytoplasmic vacuolization, atrophy, and degranulation in acinic cells. The serous acinar cells were showed autolysis and nuclear changes (pyknosis, karyorrhexis, and karyolysis). There was an increase in the interstitial spaces between each parenchymal element associated with few mononuclear cell infiltrations. The intra-lobular ducts were reduced in size and were indistinct throughout lobes. The severe changes were associated with higher desloratadine dose. Regarding biochemical analysis, the treated-groups had significantly increased serum levels of malondialdehyde, sialic acid, lactate dehydrogenase and creatin kinase, and significantly reduced serum levels of superoxide dismutase, catalase, and glutathione. Conclusion: Desloratadine administration produces noticeable histological changes in a dosedependent manner associated with increased oxidative stress markers and decreased antioxidative activity.

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Section: Discussionsupporting

confidence: 81%

Histological and Biochemical Evaluation of the Effect of Desloratadine Drug in Parotid Gland Tissues

.¹,

.²,

.³

et al. 2021

amj

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“…Some studies have shown that matrix metalloproteinases influenced the stroma of lip salivary glands, matrix proteins and basal membranes in Sjögren’s syndrome. In this study, we found that there was an increase in proteolytic activity on basal membrane and stromal proteins, most prominent change in fibronectin, laminin and type I collagen 26 . Similarly, type I collagen immunoreactivity was significantly higher in old mice and there was deterioration in acinar and ductal cells with aging.…”

Section: Discussionsupporting

confidence: 49%

Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice

Tümer

Çiçek

2018

J. Appl. Oral Sci.

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Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice.Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson’s Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2.Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001).Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging.

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“…7,8,10,16 Three studies have been conducted to associate changes in salivary gland oral mucosa with time since death. 11,12,20 Based on evaluations of the cellular changes in antemortem and post-mortem mucosa, Gururaj suggested that the decomposition process begins in the initial 24 hours, while Pradeep et al concluded that the decomposition process at the cellular level begins within 10 hours of death. 7,21 Yadav et al investigated 10 antemortem mucosa samples to determine nuclear and cytoplasmic changes at various time intervals and proposed the usefulness of histological changes in the oral mucosa for estimating PMI.…”

Section: Discussionmentioning

confidence: 99%

“…10 Due to sample size limitations, none of the aforementioned studies were able to provide statistical confirmation of the usefulness of light microscopic cellular changes in the oral mucosa as a method of predicting time since death. [9][10][11]15,20,21 Moreover, several studies either evaluated post-mortem cellular changes in the gingiva alone or in the labial mucosa. 7,8,15 No studies have yet sought to assess the role of these changes in the buccal mucosa.…”

Section: Discussionmentioning

confidence: 99%

“…7 Degenerative post-mortem changes to the epithelium and connective tissue in the oral mucosa may demonstrate specific patterns that could prove useful to determine PMI; however, few studies have sought to evaluate post-mortem cellular changes in the oral tissues including the gingiva, salivary glands and dental pulp. [7][8][9][10][11][12][13][14] Moreover, inadequacies in terms of sample size and standardised methodology have often precluded confirmation of this approach. Therefore, the current study aimed to investigate post-mortem light microscopic cellular changes in the oral tissues with the objective of estimating their potential for PMI prediction.…”

Section: Clinical and Basic Researchmentioning

confidence: 99%

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The Potential of Light Microscopic Features of the Oral Mucosa in Predicting Post-mortem Interval

Patro

Panda

Mohanty

et al. 2021

Sultan Qaboos Univ Med J

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Objectives: The post-mortem interval (PMI) refers to the amount of time elapsed between death and discovery of the body. This study aimed to evaluate light microscopic cellular changes in the oral mucosa and identify the potential of this method for predicting PMI. Methods: This prospective study was conducted between July 2016 and January 2018 at the Institute of Dental Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India. A total of 150 post-mortem (including 75 gingival and 75 buccal mucosa samples) and 40 ante-mortem (including 20 gingival and 20 buccal mucosa samples) tissue samples were compared using haematoxylin and eosin, periodic acid-Schiff (PAS) and van Gieson stains. Microscopic changes in the epithelium and connective tissue were categorised according to PMI stage as early (<12.5 hours since death), intermediate (12.5–20.5 hours since death) or late (>20.5 hours since death). Results: Most epithelial cellular changes occurred early, except for arc-shaped nuclei and epithelial shredding which were intermediate and late changes, respectively. However, microscopic changes in the connective tissue were only observable at ≥12.5 hours. There was a progressive decrease in intensity in van Gieson stains and an increase in intensity in PAS stains as PMI increased. Several microscopic features were found to be significant predictors of PMI including epithelial homogenisation, cytoplasmic vacuolation, nuclear degeneration, arc-shaped nuclei, chromatin clumping, red blood cell clumping and lysis, melanin incontinency, myofibril degeneration, salivary gland acini degeneration and epithelial connective tissue separation (P <0.050 each). Conclusion: These findings indicate that microscopic evaluation of the oral mucosa may be helpful for PMI prediction. KEYWORDS Post-mortem Changes; Light Microscopy; Oral Mucosa; Epithelial Cells; Lamina Propria; Salivary Glands; Histocytochemistry; Periodic Acid-Schiff Reaction; India.

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Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study

Cited by 5 publications

References 21 publications

Histological and Biochemical Evaluation of the Effect of Desloratadine Drug in Parotid Gland Tissues

Histological and Biochemical Evaluation of the Effect of Desloratadine Drug in Parotid Gland Tissues

Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice

The Potential of Light Microscopic Features of the Oral Mucosa in Predicting Post-mortem Interval

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