Polyspecies biofilm formation on implant surfaces with different surface characteristics

Abstract

Objective:To investigate the microbial adherence and colonization of a polyspecies biofilm on 7 differently processed titanium surfaces.Material and Methods:Six-species biofilms were formed anaerobically on 5-mm-diameter sterilized, saliva-preconditioned titanium discs. Material surfaces used were either machined, stained, acid-etched or sandblasted/acid-etched (SLA). Samples of the latter two materials were also provided in a chemically modified form, with increased wettability characteristics. Surface roughness and contact angles of all materials were determined. The discs were then incubated anaerobically for up to 16.5 h. Initial microbial adherence was evaluated after 20 min incubation and further colonization after 2, 4, 8, and 16.5 h using non-selective and selective culture techniques. Results at different time points were compared using ANOVA and Scheffé post hoc analysis.Results:The mean differences in microorganisms colonizing after the first 20 min were in a very narrow range (4.5 to 4.8 log CFU). At up to 16.5 h, the modified SLA surface exhibited the highest values for colonization (6.9±0.2 log CFU, p<0.05) but increasing growth was observed on all test surfaces over time. Discrepancies among bacterial strains on the differently crafted titanium surfaces were very similar to those described for total log CFU. F. nucleatum was below the detection limit on all surfaces after 4 h.Conclusion:Within the limitations of this in vitro study, surface roughness had a moderate influence on biofilm formation, while wettability did not seem to influence biofilm formation under the experimental conditions described. The modified SLA surface showed the highest trend for bacterial colonization.

Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing

“…Also, in another investigation, it has been shown that regardless of the titanium's surface roughness, bacterial colonization of different implant materials is quite similar over time (Schmidlin et al. ). However, we must take into account that these previous studies used culture techniques and that more than 70% of bacteria associated with peri‐implant tissue health is uncultivated (Kumar et al.…”

Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

“…18,19 There are plenty of studies in literature assessing the influence of titanium implant surface characteristics on biofilm formation. [20][21][22][23][24][25][26] In particular, increase in surface roughness and surface free energy seems to facilitate biofilm formation, although this conclusion is derived from largely descriptive literature. 11 As detailed and precise information, as it permits the direct visualization and quantification of living biofilms in their natural hydrated state.…”

Epistaxis and Staphylococcus aureus Colonization in the Nasal Vestibule

“…We may also speculate that a rough texture of crusted nasal mucosa may influence biofilm formation and facilitate nasal colonization because we know that the rough surface characteristics facilitate bacteria colonization on biotic or abiotic structures. 13 Regarding the pattern of biologic events with time, season, and even moon phase, there are numerous studies that had investigated the relation of chronobiology and epistaxis in the literature. 2,14,15 Walker et al 2 showed that seasons have an impact on the occurrence of epistaxis.…”