Effects of ClpP protease on biofilm formation of Enterococcus faecalis

Abstract

Effects of ClpP protease on biofilm formation of Enterococcus faecalis Enterococcus faecalis (E. faecalis), one of the main pathogens responsible for refractory periapical periodontitis and nosocomial infections, exhibits markedly higher pathogenicity in biofilms. Objectives: Studies have shown that caseinolytic protease P (ClpP) is involved in biofilm formation. However, to date, few studies have investigated the role of ClpP in the survival of E. faecalis, and in enhancing biofilm formation. Therefore, we investigated the role of ClpP in the formation of E. faecalis biofilms. Methodology: In our study, we used homologous recombination to construct clpP deleted and clpP complement strains of E. faecalis ATCC 29212. A viable colony counting method was used to analyze the growth patterns of E. faecalis. Crystal violet staining (CV) and confocal scanning laser microscopy (CLSM) were used to characterize biofilm mass formation and scanning electron microscopy (SEM) was used to observe the biofilm microstructure. Data was statistically analyzed via Student's t-test or one-way analysis of variance (ANOVA). Results: The results exhibited altered growth patterns for the clpP deletion strains and depleted polysaccharide matrix, resulting in reduced biofilm formation capacity compared to the standard strains. Moreover, ClpP was observed to increase biofilm formation in E. faecalis. Conclusion: Our study shows that ClpP can increase biofilm formation in E. faecalis and emphasizes the importance of ClpP as a potential target against E. faecalis.

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

“…Because a variety of bacterial species were presented in the oral cavity, microorganisms and their by-products are associated with polymicrobial EIs [ 2 , 3 ]. Particularly, Enterococcus faecalis ( E. faecalis ) related biofilms are covered in the root canals and invade into dentinal tubules, which may result in persistent infection and refractory periapical periodontitis [ 4 , 5 ]. Meanwhile, because of the complex root canal system (e.g., dentinal tubules, isthmuses, the apical delta, etc.…”

Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects

“…ClpP has been reported to play roles in stress tolerance, antimicrobial tolerance, and biofilm formation of Ef (Zheng et al 2020 ). Feng Y et al reported ClpP deletion strains inhibiting Ef’s survival and biofilm-forming (Feng et al 2021 ). PerR, a regulator of oxidative-stress response, could affect the virulence and resistance of Ef to hydrogen peroxide challenge (Verneuil et al 2005 ).…”

Pleiotropic Activity of Hop Extract: Unveiling its Antimicrobial Efficacy in Oral Cavity

“…Four representative oral pathogens were selected. Enterococcus faecalis (E. faecalis, Gram-positive) is found in tongue surface (42%), gingival groove (14%) and saliva (10%) and as the main bacterium responsible for refractory periapical inflammation, it may lead to persistent infection [23,24] . The prevalence of E. faecalis is found to be 67.5% in cases of primary periapical infection and notably higher at 89.6% in instances of secondary infection [25] .…”