Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro

Abstract

Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and gene expression in SaOS-2 cells. Material and Methods: SaOS-2 deposition of mineralizing matrix in response to increasing doses of ST (0-1000 nM) was evaluated through Alizarin Red S and Calcein Green staining techniques at 6, 12 and 24 days. Gene expression of runt-related transcription factor 2 (RUNX2), vitamin D receptor (VDR), osteopontin (SPP1) and osteonectin (ON) was analyzed by RT-PCR. Results: ST significantly influenced SaOS-2 osteogenic activity: stainings showed the presence of rounded calcified nodules, which increased both in number and in size over time and depending on ST dose. RT-PCR highlighted ST modulation of genes related to osteogenic differentiation. Conclusions: This study provided encouraging results, showing ST promoted the osteogenic commitment of SaOS-2 cells. Further studies are required to validate these data in primary osteoblasts and to investigate ST molecular pathway of action.

Viburnum opulus L. Juice Phenolic Compounds Influence Osteogenic Differentiation in Human Osteosarcoma Saos-2 Cells

“…Saos-2 cells can be differentiated to osteoblast-type cells in the presence of ascorbic acid and β-glycerol-phosphate [33]. Because the membrane-bound alkaline phosphatase enzyme is known as an early marker of osteoblast differentiation, essential for matrix mineralization, in the next step V. opulus influence on its activity was checked.…”

Evaluation of Isoflavones as Bone Resorption Inhibitors upon Interactions with Receptor Activator of Nuclear Factor-κB Ligand (RANKL)

“…Cells were cultured in DMEM-low glucose with 10% fetal bovine serum (FBS) medium supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin and 25 µg/mL amphotericin B. After 24 h osteogenesis process was induced with medium consisting of DMEM-low glucose completed with 2-phospho-l-ascorbic acid (100 µM), L-proline (34.8 µM) and β2glycerol phosphate (5 mM) [44]. On the following day, medium was changed with fresh osteogenic medium containing isoflavones for 14 days.…”

Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

“…Human osteosarcoma Saos-2 cells were cultured in DMEM Low Glucose with 10% FBS medium, supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin and 25 μg/mL amphotericin B. After 24 h, the osteogenesis process was induced with medium consisting of DMEM Low Glucose supplemented with 2-phospho- l -ascorbic acid (100 μM), l -proline (34.8 μM) and β2-glycerol phosphate (5 mM) [ 49 ]. The medium was changed every two days and contained the test compounds.…”