Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin

Abstract

Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay.Material and Methods The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05).Results All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05).Conclusion Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants.

Analysis of Oral Microbiome on Temporary Anchorage Devices Under Different Oral Hygiene Conditions

“…This led to a higher proportion of subgingival bacteria. Moreover, previous studies targeting stable and unstable TADs unveiled a slight elevation, though not signi cant, in the prevalence and quanti cation of these periodontopathic pathogens in successful TADs [31], which indicate the presence of periodontal pathogens alone might not induce signi cant changes to the local environment towards TADs.…”

Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants

“…As previously described by Andrucioli, et al 23 (2018), participants had good general and oral health, were nonsmokers and had not used antibiotics or anti-inflammatory drugs within 3 months before mini-implant removal. Two groups of mini-implants were obtained: 11 well-fixed mini-implants (successful), removed after completion of orthodontic mechanics or at the end of the treatment; and 7 unstable mini-implants, which became loose before the production of the desired tooth movement, thus being removed earlier (failed).…”

Protetores bucais esportivos: avaliação da contaminação microbiana, da rugosidade superficial e da eficácia do spray de clorexidina como método de desinfecção, em crianças

“…Streptococcus mutans 25175 a Streptococcus sobrinus As amostras provenientes dos protetores bucais foram avaliadas utilizando a técnica Checkerboard DNA-DNA Hybridization (Socransky et al,1994;Nelson-Filho et al, 2011a;Nelson-Filho et al, 2011b;Nelson-Filho et al, 2012;De Oliveira et al, 2015;Andrucioli et al, 2018;Bergamo et al, 2018;Bergamo et al, 2019). As suspensões contidas nos tubos plásticos foram fervidas em banho-maria por 10 minutos e, em seguida, neutralizadas pela adição de 800µL de acetato de amônia a A membrana foi pré-hibridada a 42ºC, por 1 hora, em solução contendo 50% formamida (Vetec Química Fina Ltda -Rio de Janeiro -RJ -Brasil), 1% de caseína (Vetec Química Fina Ltda-Rio de Janeiro -RJ -Brasil), 5x solução salina citratada (SSC) [1x SSC=150 mM NaCl (Vetec Química Fina Ltda -Rio de Janeiro -RJ -Brasil), 15M de citrato de sódio (SSC) [1 x SSC= 150mM NaCl (Vetec Química Fina Ltda -Rio de Janeiro -RJ -Brasil), 15M de citrato de sódio (J.T.Baker -Edo.…”

“…Com a finalidade de avaliar a contaminação microbiana de escovas dentais e aparelhos ortodônticos removíveis e a eficácia de diferentes soluções antissépticas na sua desinfecção, tem sido empregadas técnicas como a cultura microbiana (Batoni et al, 2001;Lessa et al, 2007;Maruo et al, 2008;Peixoto et al, 2011;Bagatin et al, 2017), técnicas de biologia molecular como o Checkerboard DNA-DNA Hybridization preconizada por Socransky (Socransky, 1994;Nelson-Filho et al, 2011a;Nelson-Filho et al, 2011b;Nelson-Filho et al, 2014;Ogawa et al, 2012;Andrucioli et al, 2018;Bergamo et al, 2019), que apresentam maior praticidade e rapidez, quando comparadas às técnicas microbiológicas convencionais. Deve-se ressaltar que técnicas que avaliam a viabilidade de células humanas por meio de ensaios colorimétricos, como o MTTtetrazólio 3-(4,5-dimetiltiazol-2-il)-2,5-difenilbrometo, preconizado por Mosmann (1983), podem ser também empregadas para avaliar a viabilidade celular bacteriana (Grela et al, 2018).…”