Proteomics characterization of Staphylococcus spp. from goat mastitis and phenogeno-typical assessment of resistance to beta-lactamics

Abstract

Mastitis occupies a prominent place among the diseases that affect dairy herds due to economic problems and public health. Staphylococcus spp. are infectious agents more involved in the etiology of caprine mastites, especially coagulase-negative Staphylococcus. Nineteen isolates of Staphylococcus spp. were obtained from subclinical caprine mastitis. All isolates were characterized by MALDI-TOF MS, being 47.36% (9/19) identified for S. epidermidis, 15.78% (3/19) for S. warneri, 10.52% (2/19) for S. aureus and S. caprae and 5.26% (1/19) for S. lugdunensis, S. simulans, and S. cohnii. All isolates characterized by MALDI-TOF were subjected a to polymerase chain reaction (PCR) for the 16S rRNA gene of Staphylococcus spp. to confirm the gender. After determining the species, tests for phenotypic detection of resistance to beta-lactams were carried out simple disk diffusion oxacillin, cefoxitin, penicillin G and amoxicillin + clavulanic acid, agar “screen” oxacillin and microdilution (MIC) cefoxitin. The disk diffusion test showed a strength of 58% (11/19) for penicillin G, 26.31% (5/19) for cefoxitin and 26.31% (5/19) for oxacillin. All strains were susceptible to amoxicillin + clavulanic acid and agar “screen” oxacillin. In the MIC, 63.15% (12/19) of the samples were cefoxitin resistant (MIC >4.0μg/ml). Then isolates were subjected to detection of the mecA resistance genes and regulators (mecl and mecRI), mecC and blaZ. Two samples of Staphylococcus epidermidis had the mecA gene. All isolates were negative for the mecA gene variant, mecl, mecRI, mecC and blaZ. These findings reinforce the importance of this group of microorganisms in the etiology of subclinical mastitis in goats and open perspectives for future research to investigate the epidemiology of the disease.

Detection of Staphylococcus Isolates and Their Antimicrobial Resistance Profiles and Virulence Genes from Subclinical Mastitis Cattle Milk Using MALDI-TOF MS, PCR and Sequencing in Free State Province, South Africa

“…Moreover, S. aureus , a bacterium that causes mastitis and is related to food-borne diseases, has also been found to be resistant to several antibiotics [ 24 ]. Additionally, the majority of methicillin-resistant S. aureus (MRSA) isolates have been detected from humans [ 25 ], livestock [ 26 ], and the environment [ 27 ]. Notably, some bacteria that cause mastitis contain genes that make them resistant to antibiotics, including the mecA gene for methicillin resistance [ 28 ].…”

Scientometrics Evaluation of Published Scientific Papers on the Use of Proteomics Technologies in Mastitis Research in Ruminants

“…Moreover, some articles studied more than one mastitis aspect. In contrast, the small number of articles on the aetiology of the infection reflects that proteomics is not widely used for pathogen identification; articles on proteomics and mastitis aetiology referred to the use of proteomics technologies (MALDI biotyping) for the identification of pathogens [ 35 , 36 , 37 ].…”

Occurrence of Multi-Resistant Staphylococcus aureus in Artisan Goat Coalho Cheese in Northeastern Brazil

“…Therefore, the multiplicity of factors associated with phenotypic resistance requires careful investigation. The detection of different genetic resistance markers, as well as the study of the regulation of gene expression, allows for a deeper understanding of the real value of their detection in predicting antimicrobial resistance, as recommended by the Clinical and Laboratory Standards Institute [19].…”