Subgingival bacterial microbiota associated with ovine periodontitis

Silva, Natália S.; Borsanelli, Ana Carolina; Júnior, Élerson Gaetti-Jardim; Schweitzer, Christiane Marie; Silveira, José Alcides Sarmento da; Bomjardim, Henrique A.; Dutra, Iveraldo S.

doi:10.1590/1678-5150-pvb-5913

Cited by 9 publications

(10 citation statements)

References 38 publications

(66 reference statements)

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“…In the present study, a high prevalence of Fusobacterium was observed in the oral microbiota of the two clinical conditions evaluated. This genus has recognised importance in the formation of dental biofilms and in recent studies its occurrence has also been reported in the oral microbiota of healthy and diseased sheep, goats and cattle with periodontitis [1, 15, 22]. In addition to being one of the most prevalent genera in animals with periodontitis, Petrimonas was also the most abundant genus among the unique genera identified in the dental microbiome of sheep with periodontitis.…”

Section: Discussionmentioning

confidence: 99%

“…In efforts to identify or associate microorganisms with the occurrence of the disease in sheep, classic studies used conventional bacterial culture of dental biofilms [9–11], until the introduction of culture-independent molecular methods [12–15]. Although these results have shown a set of potential periodontal pathogens, they have limits as a discriminatory reference for robust studies on aetiopathogenesis or even in the development and evaluation of disease control measures.…”

Section: Introductionmentioning

confidence: 99%

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Dental biofilm and its ecological interrelationships in ovine periodontitis

Borsanelli

Athayde

Agostinho

et al. 2021

Journal of Medical Microbiology

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Introduction. Periodontitis, one of the most common oral disorders in sheep, is caused by a mixed and opportunistic microbiota that severely affects the health and welfare of animals. However, little is known about the ecological processes involved and the composition of the microbiota associated with the development of the disease. Hypothesis/Gap Statement. Using high-throughput sequencing of the 16S ribosomal RNA gene and network analysis it would be possible to discriminate the microbiomes of clinically healthy sheep and those with periodontitis and possibly identify the key microorganisms associated with the disease. Aim. The present study aimed to characterise the composition of dental microbiomes and bacterial co-occurrence networks in clinically healthy sheep and animals with periodontitis. Methodology. Dental biofilm samples were collected from ten sheep with periodontitis and ten clinically healthy animals. Bacteria were identified using high-throughput sequencing of the 16S ribosomal RNA gene. Results. The most prevalent genera in the dental microbiota of sheep with periodontitis were Petrimonas , Acinetobacter , Porphyromonas and Aerococcus . In clinically healthy animals, the most significant genera were unclassified Pasteurellaceae, Pseudomonas, and Neisseria. Fusobacterium was found at high prevalence in the microbiomes of both groups. The dental microbiota of sheep in the two clinical conditions presented different profiles and the diversity and richness of bacteria was greater in the diseased animals. Network analyses showed the presence of a large number of antagonistic interactions between bacteria in the dental microbiota of animals with periodontitis, indicating the occurrence of a dysbiotic community. Through the interrelationships, members of the Prevotella genus are likely to be key pathogens, both in the dental microbiota of healthy animals and those with periodontitis. Porphyromonas stood out among the top three nodes with more centrality and the largest number of hubs in the networks of animals with periodontitis. Conclusion. The dental biofilm microbiota associated with ovine periodontitis is dysbiotic and with significant antagonistic interactions, which discriminates healthy animals from diseased animals and highlights the importance of key bacteria, such as Petrimonas , Porphyromonas , Prevotella and Fusobacterium species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dental biofilm and its ecological interrelationships in ovine periodontitis

Borsanelli

Athayde

Agostinho

et al. 2021

Journal of Medical Microbiology

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“…Fusobacterium is considered an important "bridging" species in the subgingival ecosystem, facilitating species co-aggregation in the formation and maturation of dental biofilm. In animals, Fusobacterium has already been identified in cats and dogs with gingivitis and periodontitis (Dewhirst et al, 2012;Harris et al, 2015) as well as in sheep, goats, and cattle with periodontitis (Dutra et al, 2000;Campello et al, 2019;Silva et al, 2019;Borsanelli et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

et al. 2022

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Extensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.

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“…Periodontitis affecting sheep was firstly reported in sheep by Döbereiner et al (1974). Currently, it was described in the North (Silva et al 2016(Silva et al , 2019, Southeast (Borsanelli et al 2017) and Northeast (Wicpolt et al 2022) Brazilian regions.…”

Section: Introductionmentioning

confidence: 99%

“…Periodontitis is associated with a multispecies biofilm, in which bacteria are essential but insufficient for developing the inflammatory process (West & Spence 2000). Among the bacteria most reported as potential periodontal agents in sheep, members of Fusobacterium, Treponema, Tannerella, Porphyromonas, Prevotella and, Petrimonas genera were identified by culture-independent methods (Borsanelli et al 2017, 2021, Silva et al 2019. The imbalance and polymicrobial synergism affect the biofilm ecology and result in loss of oral homeostasis (Hajishengallis 2015).…”

Section: Introductionmentioning

confidence: 99%

Pathology of chronic ovine periodontitis

et al. 2022

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Periodontitis is an inflammatory process of infectious origin affecting the teeth and their supporting structures, causing significant economic losses and reducing animal welfare. Bacteria in the gingival biofilm are one of the main factors in initiating inflammatory lesions. Bacteria act directly on tissues or indirectly through substances that cause tissue damage. Studies on the etiopathogenesis of periodontitis in Brazilian sheep herds are scarce. The present study aimed to characterize histologically periodontal lesions of culled sheep from the Brazilian breed, Santa Inês. Periodontal lesions, such as periodontal pockets containing plant tissue and bacteria, replacement of the periodontal ligament by connective tissue and inflammatory cells, superficial pustules, hydropic epithelial degeneration, and epithelium hyperplasia, were observed. Submucosal changes were characterized by granulation tissue, edema, swelling of the endothelial cells, bacteria, and predominantly perivascular lymphoplasmacytic inflammatory infiltrate. In the alveolar bone, osteoclastic resorption and bone apposition were observed. This study revealed subacute to chronic inflammation, alveolar bone resorption, and cortical bone apposition in ovine periodontitis. Thus, these findings can contribute to the evolution of knowledge about the etiopathogenesis of ovine periodontitis and, possibly, the development of measures to control the disease.

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Subgingival bacterial microbiota associated with ovine periodontitis

Cited by 9 publications

References 38 publications

Dental biofilm and its ecological interrelationships in ovine periodontitis

Dental biofilm and its ecological interrelationships in ovine periodontitis

Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

Pathology of chronic ovine periodontitis

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