Detection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Brazilian mastitic milk goats by multiplex-PCR

Abstract

This study evalueted the prevalence of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in milk samples from 257 goats (513 half-udders) and ten bulk tanks, from ten dairy goat farms of São Paulo State, Brazil, by multiplex-PCR. The samples were screened by microbiological culture (gold-standard), and tested by different multiplex-PCR protocols for the detection of each bacterium. A total of 178 half-udders resulted positive by microbiological culture, with coagulase-negative staphylococci (70%), S. aureus (13.5%), S. intermedius (7.9%), and Enterobacteriaceae (4%) the prevalent pathogens. In other way, multiplex-PCR detected 173 pathogens in 151/523 (28.9%; CI95% 25.2-32.9%) milk samples 144/513 (28.1%) half-udders and 7/10 (70%) bulk tanks, with E. coli (86/162, 51.9%) and S. aureus (50/162, 30.9%) the prevalent ones in half-udders, and S. aureus (6/10, 60%) and E. coli (4/5, 36.4%) in bulk tanks. Multiplex-PCR showed a high performance for the detection of three bacteria at a time in mastitic goat milk direct from half-udders or bulk tanks. Thus, this multiplex-PCR protocol proved to be an adequate tool for the identification of the most common mastitis pathogens, independent of their phenotypic characteristics in the diagnosis of clinical mastitis in goats, allowing a continuous and better vigilance and monitoring the herd, being included in quality programs.

β-lactam resistance in bacteria associated with subclinical mastitis in goats in Thika Subcounty, Kenya

“…Enterobacteriaceae are also associated with mastitis [41]. We isolated a relatively high proportion of members of the Enterobacteriaceae family (55.5%) in our study, which is comparable to a previous report of the isolation of 64.5% Enterobacteriaceae from milk samples of goats with mastitis in Thika East subcounty [21].…”

Phenotypic and Molecular Characterization of <i>Staphylococcaceae</i> and <i>Enterobacteriaceae</i> Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou

“…The use of staur primers allowed the amplification of 23S rDNA specific regions in most tested strains, thus confirming that they are Staphylococcus aureus species. This primer has been used in several studies for the identification of Staphylococcus aureus isolates [115] [116]. However, the nuc gene was detected in 86% of the Staphylococcus aureus strains tested.…”

mastitis-in-small-ruminants

“…The pathogens identified are Staphylococcus spp. (S. aureus, S. caprae, S. epidermidis, S. saprophyticus, S. hyicus, S. intermedius, S. simulans, S. equorum, S. capitis, S. lentus, S. gallinarum and S. xylosus [4,19,20], M. capricolum, M. putrefaciens and S. equi subspecies ruminatorum [21,22] and less frequently Mycoplasma agalactiae, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Brucella spp., Mycobacterium spp., Yersinia pseudotuberculosis, Coxiella burnetti, Mannheimia haemolytica, Corynebacterium spp., Yersinia pseudotuberculosis and Nocardia spp., As well as fungi and yeasts: Candida albicans, Aspergillus fumigatus, Aspergillus terreus, Cryptococcus albidus, Cryptococcus neoformans, Rhodotorula glutinis and Geotrichum candidum [10,11,14,18,23], besides the algae [5].…”