Development and identification of an elite wheat-Hordeum californicum T6HcS/6BL translocation line ND646 containing several desirable traits

Abstract

Hordeum californicum ( H. californicum , 2n=2X=14, H c H c ), one of the wild relatives of wheat ( Triticum aestivum L.), harbors many desirable genes and is a potential genetic resource for wheat improvement. In this study, an elite line ND646 was selected from a BC 4 F 5 population, which was developed using 60 Co-γ irradiated wheat- H. californicum disomic addition line WJ28-1 (DA6H c ) as the donor parent and Ningchun 4 as the recurrent parent. ND646 was identified as a novel wheat- H. californicum 6H c S/6BL translocation line using genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and H. californicum -specific expressed sequence tag (EST) markers. Further evaluation revealed that ND646 had excellent performance in several traits, such as a higher sedimentation value (SV), higher water absorption rate (WAR), and higher hardness index (HI). More importantly, it had more kernels per spike (KPS), a higher grain yields (GY), and good resistance to powdery mildew, leaf rust, and 2,4-D butylate (2,4-D). Its excellent phenotypic performance laid the foundation for further investigation of its genetic architecture and makes ND646 a useful germplasm resource for wheat breeding.

The Genomic Variation and Differentially Expressed Genes on the 6P Chromosomes in Wheat–Agropyron cristatum Addition Lines 5113 and II-30-5 Confer Different Desirable Traits

“…Transferring desirable genes from wild relatives into common wheat is an effective approach for broadening wheat genetic diversity [ 3 ]. Many alien genes have been transferred into common wheat through chromosome translocations from its wild relatives, such as Secale cereale [ 4 ], Hordeum californicum [ 5 ], Thinopyrum [ 6 , 7 ], and Aegilops peregrina [ 8 , 9 ]. The 1RS chromosome of rye has been successfully introduced into wheat through a rye–wheat 1RS.1BL translocation line, and its resistance genes Pm8 , Lr26 , and Yr9 have been widely used in wheat breeding [ 4 , 10 , 11 , 12 ].…”