In silico comparisons of lipid-related genes between Mycobacterium tuberculosis and BCG vaccine strains

Sarno, Alice; Bitencourt, Júlia Valéria de Oliveira Vargas; Queiroz, Adriano; Arruda, Sérgio

doi:10.1590/1678-4685-gmb-2021-0024

Cited by 1 publication

(1 citation statement)

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“…Recently, we performed an in silico evaluation and found 14 nonhomologous lipid-related genes absent in the six BCG strains most used worldwide relative to Mtb . Those genes were associated with the functional categories “cell wall and cell processes”, “virulence, detoxification and adaptation”, “lipid metabolism”, and “intermediary metabolism and respiration”, and together, these gene modifications may favor a dormant-like state of the BCG strains ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Impaired macrophage and memory T-cell responses to Bacillus Calmette-Guerin nonpolar lipid extract

Sarno,

Leite,

Augusto

et al. 2024

Front. Immunol.

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IntroductionThe attenuation of BCG has led to the loss of not only immunogenic proteins but also lipid antigens.MethodsThus, we compared the macrophage and T-cell responses to nonpolar lipid extracts harvested from BCG and Mycobacterium tuberculosis (Mtb) to better understand the role of BCG lipids in the already known diminished responses of the vaccine strain.ResultsRelative to Mtb, nonpolar lipid extract from BCG presented a reduced capacity to trigger the expression of the genes encoding TNF, IL-1b, IL-6 and IL-10 in RAW 264.7 macrophages. Immunophenotyping of PBMCs isolated from healthy individuals revealed that lipids from both BCG and Mtb were able to induce an increased frequency of CD4+ and CD8+ T cells, but only the lipid extract from Mtb enhanced the frequency of CD4-CD8-double-negative, γσ+, CD4+HLA-DR+, and γσ+HLA-DR+ T cells relative to the nonstimulated control. Interestingly, only the Mtb lipid extract was able to increase the frequency of CD4+ memory (CD45RO+) T cells, whereas the BCG lipid extract induced a diminished frequency of CD4+ central memory (CD45RO+CCR7-) T cells after 48 h of culture compared to Mtb.DiscussionThese findings show that the nonpolar lipids of the BCG bacilli presented diminished ability to trigger both proinflammatory and memory responses and suggest a potential use of Mtb lipids as adjuvants to increase the BCG vaccine efficacy.

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Section: Introductionmentioning

confidence: 99%