Palmitic acid decreases cell migration by increasing RGS2 expression and decreasing SERCA expression

Galindo-Hernández, Octavio; Leija-Montoya, Ana Gabriela; Romero‐García, Tatiana; Vazquez-Jimenez, J. Gustavo

doi:10.1590/1678-4685-gmb-2020-0279

Cited by 5 publications

(5 citation statements)

References 24 publications

(10 reference statements)

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“…6h ), suggesting that aromatase repressed RGS2 activity. As expected, overexpression of RGS2 significantly inhibited JEG-3 cell migration and invasion, which was consistent with previous reports 32 , 33 , while coexpression of aromatase effectively attenuated the RGS2-suppressed migratory and invasive capacities of JEG-3 cells (Fig. 6i–k ).…”

Section: Resultssupporting

confidence: 92%

RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy

Tang

Jin

et al. 2023

Exp Mol Med

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Production of estradiol (E2) by the placenta during human pregnancy ensures successful maintenance of placental development and fetal growth by stimulating trophoblast proliferation and the differentiation of cytotrophoblasts into syncytiotrophoblasts. Decreased levels of E2 are closely associated with obstetrical diseases such as preeclampsia (PE) in the clinic. However, the mechanisms underlying the inhibition of placental E2 biosynthesis remain poorly understood. Here, we report that regulator of G-protein signaling 2 (RGS2) affects E2 levels by regulating aromatase, a rate-limiting enzyme for E2 biosynthesis, by using human trophoblast-derived JEG-3 cells and human placental villus tissues. RGS2 enhanced the protein degradation of the transcription factor heart and neural crest derivatives expressed 1 (HAND1) by suppressing ubiquitin-specific protease 14 (USP14)-mediated deubiquitination of HAND1, resulting in the restoration of HAND1-induced trans-inactivation of the aromatase gene and subsequent increases in E2 levels. However, aromatase bound to RGS2 and repressed RGS2 GTPase activating protein (GAP) activity. Moreover, we observed a positive correlation between RGS2 and aromatase expression in clinical normal and preeclamptic placental tissues. Our results uncover a hitherto uncharacterized role of the RGS2-aromatase axis in the regulation of E2 production by human placental trophoblasts, which may pinpoint the molecular pathogenesis and highlight potential biomarkers for related obstetrical diseases.

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Section: Resultssupporting

confidence: 92%

RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy

Tang

Jin

et al. 2023

Exp Mol Med

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“…There is limited evidence that supports or refutes oleic and palmitic acid's role on neuronal migration. Both fatty acids can either stimulate or inhibit migration and may be dependent on the concentration and the cellular subtype examined [89][90][91]. The results from this study show oleic acid to reduce neuronal migration with no effect by palmitic acid.…”

Section: High Fat Diet-induced Neurogenesis Is Partially Mediated By ...mentioning

confidence: 66%

Concentration-dependent change in hypothalamic neuronal transcriptome by the dietary fatty acids: oleic and palmitic acids

Valencia¹,

Marino²,

Noutsos³

et al. 2022

The Journal of Nutritional Biochemistry

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“…There is limited evidence that supports or refutes oleic and palmitic acid’s role on neuronal migration. Both fatty acids can either stimulate or inhibit migration and may be dependent on the concentration and the cellular subtype examined [89–91]. The results from this study show oleic acid to reduce neuronal migration with no effect by palmitic acid.…”

Section: Discussionmentioning

confidence: 90%

Concentration-dependent change in hypothalamic neuronal transcriptome by the dietary fatty acids: oleic and palmitic acids

Valencia

Mariño

Noutsos

et al. 2021

Preprint

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Prenatal high-fat diet exposure increases hypothalamic neurogenesis events in embryos and programs offspring to be obesity-prone. The molecular mechanism involved in these dietary effects of neurogenesis are unknown. This study investigated the effects of oleic and palmitic acids, which are abundant in a high-fat diet, on the hypothalamic neuronal transcriptome and how these changes impact neurogenesis events. The results show differential effects of low and high concentrations of oleic or palmitic acid treatment on differential gene transcription. Gene ontology analysis uncovered significant gene enrichment in several cellular pathways involved in gene regulation and protein production, particularly with proliferation, migration, and cell survival. The enriched signaling pathways include Wnt, integrin, PDGF, and apoptosis, in addition endocrine function signaling pathways CCKR and GnRH. Further examination of proliferation and migration show low concentrations of oleic acid to stimulate proliferation and high concentrations of both oleic and palmitic acid to stimulate apoptosis. Oleic acid also reduced hypothalamic neuronal migration, with little effects by palmitic acid. The results show direct impact of the two most abundant fatty acids in a high fat diet to directly impact hypothalamic neuronal proliferation and migration. The results also uncovered signaling pathways affected by oleic and palmitic acid and suggest a mechanism of prenatal high-fat diet induced neurogenesis events is through these two abundant fatty acids.

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Palmitic acid decreases cell migration by increasing RGS2 expression and decreasing SERCA expression

Cited by 5 publications

References 24 publications

RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy

RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy

Concentration-dependent change in hypothalamic neuronal transcriptome by the dietary fatty acids: oleic and palmitic acids

Concentration-dependent change in hypothalamic neuronal transcriptome by the dietary fatty acids: oleic and palmitic acids

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