Genomic sequencing of different sequevars of Ralstonia solanacearum belonging to the Moko ecotype

Abstract

Banana vascular wilt or Moko is a disease caused by Ralstonia solanacearum . This study aimed to sequence, assemble, annotate, and compare the genomes of R. solanacearum Moko ecotypes of different sequevar strains from Brazil. Average nucleotide identity analyses demonstrated a high correlation (> 96%) between the genome sequences of strains CCRMRs277 (sequevar IIA-24), CCRMRs287 (IIB-4), CCRMRs304 (IIA-24), and CCRMRsB7 (IIB-25), which were grouped into phylotypes IIA and IIB. The number of coding sequences present in chromosomes and megaplasmids varied from 3,070 to 3,521 and 1,669 to 1,750, respectively. Pangenome analysis identified 3,378 clusters in the chromosomes, of which 2,604 were shared by all four analyzed genomes and 2,580 were single copies. In megaplasmids, 1,834 clusters were identified, of which 1,005 were shared by all four genomes and 992 were identified as single copies. Strains CCRMRsB7 and CCRMRs287 differed from the others by having unique clusters in both their chromosomes and megaplasmids, and CCRMRsB7 possessed the largest genome among all Moko ecotype strains sequenced to date. Therefore, the genomic information obtained in this study provides a theoretical basis for the identification, characterization, and phylogenetic analysis of R. solanacearum Moko ecotypes.

A pangenomic atlas reveals that eco-evolutionary dynamics shape plant pathogen type VI secretion systems

“…Pioneering studies on Moko disease by Luis Sequeira and Ivan Buddenhagan demonstrated that the causal pathogens are facultatively transmitted by either soil transmission or contact transmission of insects with sap, similar to BDB (86,87). We and other groups have sequenced isolates from the Sequiera and Buddenhagan collection from the 1960s Moko epidemic (88)(89)(90)(91), and we now know that multiple RSSC lineages were responsible for the epidemic, including T6SS + lineages (IIB-4 and IIB-3) and T6SS null lineages (IIA-6). Further epidemiological research is needed to understand how variation in transmission routes shapes the evolution and behavior of RSSC lineages.…”

A Meta-analysis of the known Global Distribution and Host Range of theRalstoniaSpecies Complex

“…To get a better estimate of the diversity of potential hosts for Ralstonia, we did a search of the literature using search terms like "weeds", "host range", and older names for the pathogen (Pseudomonas solanacearum, Burkholderia solanacearum). [189][190][191][192][193] 2022 [194][195][196][197][198] Converting strain metadata into a structured format for data science:…”

Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants

“…In spite of Moko ecotype identification (Albuquerque et al, 2014(Albuquerque et al, , 2021a and genomic characterization of representative strains by Silva et al (2020) and Pais et al (2021), no genomic studies have looked the different symptomatology caused by the R. solanacearum Moko ecotype. Therefore, the objective of this study was to investigate the representatives of the Moko ecotype that induce different symptoms in banana trees using comparative genomics and phylogenomic approaches.…”