Diagnostic value of miR-145 and its regulatory role in macrophage immune response in tuberculosis

Abstract

Tuberculosis (TB) induced by Mycobacterium tuberculosis (Mtb) is a serious global health burden. This study sought to investigate the expression and diagnostic value of serum miR-145 in TB patients and explore the biological function of miR-145 using macrophages. Serum expression levels of miR-145 were estimated by quantitative real-time PCR. A receiver operating characteristic curve was plotted to evaluate the diagnostic accuracy of miR-145. This study further focused on the effects of miR-145 on cell viability and inflammation in macrophages upon Mtb infection, and explored the potential target gene of miR-145. Serum expression levels of miR-145 were decreased in TB patients, and the upregulated inflammatory cytokines in TB patients were negatively correlated with the serum expression levels of miR-145. miR-145 had considerable diagnostic accuracy in distinguishing of TB patients from healthy individuals and differentiating between active TB cases and latent TB cases. Mtb infection induced an increase in cell viability and inflammatory responses in macrophages, but these promoting effects were rescued by the overexpression of miR-145. CXCL16 was determined as a target gene of miR-145 in macrophages. Overall, this study demonstrated that the decreased serum miR-145 expression serves a candidate diagnostic biomarker in TB patients. The overexpression of miR-145 in macrophages upon Mtb infection can suppress cell viability and infection-induced inflammation via regulating CXCL16, indicating the potential of miR-145 as a therapeutic target of TB.

circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

“…miR-145 regulates the polarization of macrophage through IL-16 [ 24 ]. miR-145 also regulates macrophage immune response in tuberculosis [ 25 ]. In our research, we found that miR-145 was the downstream of circCOL1A1.…”

The Role of Extracellular Vesicles from Human Macrophages on Host-Pathogen Interaction

“…In addition, higher sensitivity of sMR was found to better predict mortality than other inflammatory markers such as procalcitonin (PCT), C-reactive protein (CRP), and sCD163 [ 104 ]. Serum expression levels of miR-145 from macrophages were observed to distinguishing Mtb-infected patients from healthy individuals and differentiating between active and latent tuberculosis cases [ 105 ]. Similarly, macrophages containing SARS-CoV-2 viral particles were found to express high levels of IL-6, and the presence of this inflammatory mediator was associated with severe depletion of lymphocytes from the spleen and lymph nodes in severe Coronavirus disease 2019 (COVID-19) patients [ 106 ].…”

miR-29 as diagnostic biomarkers for tuberculosis: a systematic review and meta-analysis

“…Furthermore, miRNA has been employed as a biological indicator to differentiate between latent and active TB (7) and as a biomarker for TB coinfection in individuals with HIV (8). Recent studies have also showcased the significant role of these biomarkers in predicting the progression of the disease (9). In prior investigations, microarray technology has been employed in multiple studies to pinpoint miRNAs as potential biomarkers for TB in multiple sample sources, such as serum, blood, sputum macrophages, and peripheral blood mononuclear cells of TB patients (10)(11)(12).…”