Selenium protects against LPS-induced MC3T3-E1 cells apoptosis through modulation of microRNA-155 and PI3K/Akt signaling pathways

Huang, Yan; Jia, Zhen; Xu, Yongqiang; Qin, MeiLan; Feng, Siyin

doi:10.1590/1678-4685-gmb-2019-0153

Cited by 13 publications

(10 citation statements)

References 31 publications

(24 reference statements)

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“…As a result of treatment with SeNPs and H 2 O 2 together, cell viability increased compared to the untreated group when selenium was applied, and the SeNP-treated group showed an approximately 17.24–39.58% increase in cell viability compared to the SeNP-untreated group, when 300 μM H 2 O 2 was treated ( Figure 3 d). This confirmed the cell viability at a relatively higher selenium concentration compared to previous studies that treated MC3T3-E1 cells with selenium at 0–800 ng/mL [ 60 ]. The highest concentration of 20 μg/mL SeNPs and 200 μM H 2 O 2 was used for further experiments, and we analyzed the effect of SeNPs on ROS in H 2 O 2 .…”

Section: Resultssupporting

confidence: 87%

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Lee

Patel

et al. 2021

Nanomaterials

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Reactive oxygen species (ROS) regulate various functions of cells, including cell death, viability, and differentiation, and nanoparticles influence ROS depending on their size and shape. Selenium is known to regulate various physiological functions, such as cell differentiations and anti-inflammatory functions, and plays an important role in the regulation of ROS as an antioxidant. This study aims to investigate the effect of selenium nanoparticles (SeNPs) on the differentiation of osteogenic MC3T3-E1 cells. After fabrication of SeNPs with a size of 25.3 ± 2.6 nm, and confirmation of its oxidase-like activity, SeNPs were added to MC3T3-E1 cells with or without H2O2: 5~20 μg/mL SeNPs recovered cells damaged by 200 μM H2O2 via the intracellular ROS downregulating role of SeNPs, revealed by the ROS staining assay. The increase in osteogenic maturation with SeNPs was gradually investigated by expression of osteogenic genes at 3 and 7 days, Alkaline phosphatase activity staining at 14 days, and Alizarin red S staining at 28 days. Therefore, the role of SeNPs in regulating ROS and their therapeutic effects on the differentiation of MC3T3-E1 cells were determined, leading to possible applications for bone treatment.

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Section: Resultssupporting

confidence: 87%

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Lee

Patel

et al. 2021

Nanomaterials

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“…As an infectious bone disease, COM is a dysregulation of bone formation and resorption, which results in excessive bone resorption. Akt signaling is critical for osteoclast differentiation and apoptosis, which is a vital part of osteomyelitis [ 32 , 33 ]. MMP9 is an osteoclast-specific gene and involves in the degradation of the bone matrix during bone resorption [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-Based Analysis on Lonicera japonica for Chronic Osteomyelitis Treatment

Shao

Huang

2022

Journal of Oncology

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Ethnopharmacological Relevance. Lonicera japonica (LJP) is a broadly used traditional Chinese medication treatment for chronic osteomyelitis (COM). But, the main antiosteomyelitis compounds and functional targets of LJP are still unclear. Aim of the Study. To screen LJP drug targets and active compounds in COM treatment. Materials and Methods. Active compounds of LJP were examined established on the analysis platform, Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. DrugBank identified drug targets and annotated them on UniPort and GeneCards. Besides, the COM-related genes were identified on GeneCards. The network of the drug, main active compounds, targets, and diseases was built utilizing Cytoscape. STRING was utilized to build the protein-protein interaction network. Moreover, the KEGG and GO pathway enrichment analysis were applied to analyze biological function. Results. 23 active compounds of LJP were screened, and 204 drug targets and 686 COM-related genes were identified. Forty-five intersection genes were overlapped from 204 drug targets and 686 COM-related genes. The drug-active compounds-target protein-diseases network was established based on 23 active compounds of LJP and 45 intersection genes. Moreover, the interaction of 45 intersection genes was explored by the PPI network, and the drug-active compounds-target protein-diseases network was formed grounded by 23 active compounds of LJP, 45 intersection genes, and PPI network. The KEGG and GO pathway enrichment analysis specified that 45 intersection genes primarily enriched in immune-related pathways and oxidative stress-related pathways. Conclusions. In the research done, the main active compounds of LJP and drug targets in the treatment of COM were identified. Our findings might provide the ingredient option of LJP and drug targets of LJP in COM treatment.

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“…Activation of PI3K/AKT pathway contributes to fracture healing 25 . Additionally, the AKT pathway is involved in osteoblastic osteogenesis of MC3T3‐E1 cells 26,27 . The expression of AKT3 was upregulated in osteoarthritis chondrocytes 28 .…”

Section: Discussionmentioning

confidence: 99%

lncRNA MALAT1 promotes osteogenic differentiation through the miR‐217/AKT3 axis: A possible strategy to alleviate osteoporosis

Song

Guo

Chen

et al. 2022

The Journal of Gene Medicine

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Background: Accumulating evidence demonstrates that long non-coding RNAs (lncRNAs) are associated with the development of osteoporosis. The present study aimed to investigate the effect of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on osteogenic differentiation in osteoporosis.Methods: MALAT1 levels were detected by a real-time polymerase chain reaction (RT-qPCR). Moreover, the levels of osteogenic differentiation-related factors (Bmp4, Col1a1 and Spp1) were measured by a RT-qPCR and western blotting. Alkaline phosphatase (ALP) activity was detected using an ALP staining assay.Results: MALAT1 levels were downregulated in hindlimb unloading mice and simulated in microgravity (MG) treated MC3T3-E1 cells. Moreover, MG treatment induced the downregulation of the expression of ALP, BMP4, Col1a1 and Spp1, whereas overexpression of MALAT1 abolished the downregulation. MG also inhibited ALP activity, whereas MALAT1 reversed the effect. Furthermore, miR-217 was identified as a target of MALAT1, and AKT3 was verified as a target of miR-217.Overexpression of miR-217 rescued the promotion of osteogenic differentiation induced by MALAT1 in MG treated cells. Knockdown of AKT3 abolished the facilitation of osteogenic differentiation induced by downregulation of miR-217.Conclusions: MALAT1 promotes osteogenic differentiation through regulating miR-217/AKT3 axis, suggesting that MALAT1 is a potential target with respect to alleviating osteoporosis.

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Selenium protects against LPS-induced MC3T3-E1 cells apoptosis through modulation of microRNA-155 and PI3K/Akt signaling pathways

Cited by 13 publications

References 31 publications

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Network Pharmacology-Based Analysis on Lonicera japonica for Chronic Osteomyelitis Treatment

lncRNA MALAT1 promotes osteogenic differentiation through the miR‐217/AKT3 axis: A possible strategy to alleviate osteoporosis

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