Underrated Staphylococcus species and their role in antimicrobial resistance spreading

Rossi, Ciro César; Pereira, Monalessa Fábia; Giambiagi-deMarval, Márcia

doi:10.1590/1678-4685-gmb-2019-0065

Cited by 54 publications

(35 citation statements)

References 110 publications

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“…nepalensis strains studied in this work are resistant to multiple drugs [7], and the animals from which they were isolated had not been under antimicrobial therapy, it is possible that they received these genetic resistance determinants from other species of Staphylococcus they may have had contact with. Growing evidence, including that of the present study, indicates that bacteria of the genus Staphylococcus can supply each other with multiple-resistance plasmids [25].…”

Section: Discussionmentioning

confidence: 75%

Staphylococcus nepalensis, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance

et al. 2020

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Staphylococcus nepalensis is a commensal bacterium from the oral microbiota of domestic cats, with a still obscure clinical importance. In this work, we analysed the ability of feline strains of S. nepalensis to transfer antimicrobial resistance genes to Staphylococcus aureus isolated from humans through plasmids. To this end, we first analysed all publicly available genomes from cat staphylococci using computational methods to build a pan-resistome. Genes that encode resistance to erythromycin, gentamicin, mupirocin and tetracycline, common to human and cat staphylococci and previously described to be located in mobile genetic elements, were chosen for the next analyses. We studied 15 strains of S. nepalensis , which were shown to be genetically different by GTG5-PCR. As observed by disc diffusion, resistance to tetracycline was widespread (80 %), followed by resistance to erythromycin (40 %), gentamicin (27 %) and mupirocin (7 %). The strains were positive for several antimicrobial resistance genes and more than half of them harboured plasmids. The loss of plasmids and resistance genes in some strains were induced by stress with SDS. Through conjugation experiments, we observed that these plasmids can be transferred to S. aureus , thus increasing its potential to resist drug therapy. Our findings show that S. nepalensis , an underestimated inhabitant of the cat microbiota, can be a reservoir of antimicrobial resistance genes for S. aureus and, like many other staphylococci, be an overlooked and silent threat to their animal hosts and humans living with them.

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Section: Discussionmentioning

confidence: 75%

Staphylococcus nepalensis, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance

et al. 2020

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“…Bacillus toyoniensis was isolated from South Africa marine sediment by Ugbenyen et al (2017) for the production of flocculant used in the biodegrading of pollutants. Staphylococcus spp belonging to Firmicutes occurs ubiquitously in nature and have been isolated from various animals such as birds and mammals (Rossi et al, 2020). Staphylococcus xylosus and S. succinus identified in this study have been used in Italy for the fermentation of traditional sausages (Ratsimba et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Kiama¹,

Njire²,

Kambura³

et al. 2021

Afr. J. Microbiol. Res.

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There is still unexplored reservoir of microorganisms from sediments and water within Lakes Olbolosat and Oloiden using culture dependent technique. The current study compares bacterial diversity within Lake Olbolosat a freshwater lake and Lake Oloiden a saline alkaline lake. Out of 60 isolates obtained from sediments and water samples, 35 were from Lake Olbolosat and 25 from Lake Oloiden. Microbial count ranged between 0-1.75× 10 5 cfu/ml from both lakes. There was a significant difference between bacterial density and sampling points (p<0.001, F=6.667), 58 were Gram-positive and 2 Gram-negative. Fifty-five isolates that were rod-shaped, 3 were cocci and 2 filamentous. There was excellent growth of isolates at an optimum growth pH range of 6-10, a temperature range of 25-30°C and a salinity range of 0-5%. There was significant difference at p<0.001 for bacterial growth within physiological parameters. The isolates utilized skimmed milk, starch, olive oil, cellulose powder and xylan, hence the production of extracellular enzymes. There was antimicrobial activity against Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Candida albicans by bacterial isolates. BLAST analysis of partial sequences showed there were 4 different phyla. Firmicutes scored 77% closely affiliated with 20 strains, Actinobacteria scored 15% closely affiliated with 4 strains, Proteobacteria and Bacteroidetes scored 4% affiliated with 1 strain each. Novel bacteria from this study could provide insights into their diversity and biotechnological applications.

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“…Coagulase-negative staphylococci (CoNS) have so far been considered as contaminants or commensals with minimum or no clinical significance (except when isolated from medical and healthcare device-related infections). The emergence and spread of antimicrobial resistance and growing evidence suggesting the transfer of resistance elements among staphylococci (including S. aureus and MRSA) make this group of organisms important to identify and study further [9]. While a majority of the studies report S. epidermidis as the most common (~75%) CoNS species among bacteremia and hospital-acquired infection (HAI) cases [10], in our experience, the majority of the CoNS isolates with clinical significance are S. haemolyticus followed by S. epidermidis [11].…”

Section: Discussionmentioning

confidence: 99%

Accuracy of different methods for identification of Staphylococcus haemolyticus

Manoharan¹,

Sistla²,

Ray³

2021

Journal of Microbiology and Infectious Diseases

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Objectives: Staphylococcus haemolyticus is associated with device-related infections in immunocompromised individuals and acts as a reservoir for antibiotic resistance genes. It is also the species with the highest antibiotic resistance rates. However, identification is still difficult in most clinical laboratories. Simplified biochemical tests give variable results while newer methods such as MALDI-TOF MS and automated systems may not be readily available. Aim: To compare the performance of the simplified biochemical scheme, BD-Phoenix automated system, and PCR for nuc gene for the identification of S. haemolyticus with MALDI-TOF MS as the gold standard. Methods: This study included 427 coagulase-negative staphylococci (CoNS) isolates of which 356 were identified as S. haemolyticus and 71 as other species by MALDI-TOF MS. These isolates were subjected to a simplified biochemical scheme using tests like the fermentation of maltose, sucrose, trehalose, mannose, urease, xylose, ornithine, and susceptibility to novobiocin. Conventional PCR targeting the nuc gene and BD-Phoenix were also used for identification. The accuracy of these methods was assessed in comparison with MALDI-TOF MS. Results: The sensitivity and specificity of biochemical tests, BD-Phoenix and nuc PCR were 97.5% and 97.2%: 97.8% and 100%: 100% and 100% respectively. Inaccurate identification was observed for some of the isolates (2.2% by BD-Phoenix and 2.5% by biochemical tests). These isolates were identified as S. haemolyticus by the other methods. Conclusion: Identification of S. haemolyticus by biochemical tests and BD-Phoenix had good accuracy comparable to PCR as well as MALDI-TOF MS. This simplified biochemical scheme can be easily implemented even in laboratories with limited resources.

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Underrated Staphylococcus species and their role in antimicrobial resistance spreading

Cited by 54 publications

References 110 publications

Staphylococcus nepalensis, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance

Staphylococcus nepalensis, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance

Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya

Accuracy of different methods for identification of Staphylococcus haemolyticus

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