Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses

Pang, Xinyue; Xue, Min; Ren, Maozhi; Nan, Dina; Wu, Yaqi; H, Guo

doi:10.1590/1678-4685-gmb-2018-0101

Cited by 18 publications

(7 citation statements)

References 53 publications

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“…In addition, overexpression of ANAC069 can decrease plant ROS scavenging capability and proline biosynthesis, while knock down of ANAC069 improves plant salt and osmotic stress tolerance [ 36 ], indicating ANAC069 as a negative regulator of plant stress tolerance. In Arabidopsis, the homologous gene of PsnNAC036 is ANAC07 2 (AT4G27410.2, RD26 ), which has been reported to respond to drought, salinity, ABA, SA, and MeJA [ 37 , 38 ]. In this study, we provide several lines of evidence, showing that the PsnNAC036 can improve plant salinity and heattolerance.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of PsnNAC036 under Salinity and High Temperature Stresses

Zhang

Cheng

Yao

et al. 2021

IJMS

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Plant growth and development are challenged by biotic and abiotic stresses including salinity and heat stresses. For Populus simonii × P. nigra as an important greening and economic tree species in China, increasing soil salinization and global warming have become major environmental challenges. We aim to unravel the molecular mechanisms underlying tree tolerance to salt stress and high temprerature (HT) stress conditions. Transcriptomics revealed that a PsnNAC036 transcription factor (TF) was significantly induced by salt stress in P. simonii × P. nigra. This study focuses on addressing the biological functions of PsnNAC036. The gene was cloned, and its temporal and spatial expression was analyzed under different stresses. PsnNAC036 was significantly upregulated under 150 mM NaCl and 37 °C for 12 h. The result is consistent with the presence of stress responsive cis-elements in the PsnNAC036 promoter. Subcellular localization analysis showed that PsnNAC036 was targeted to the nucleus. Additionally, PsnNAC036 was highly expressed in the leaves and roots. To investigate the core activation region of PsnNAC036 protein and its potential regulatory factors and targets, we conducted trans-activation analysis and the result indicates that the C-terminal region of 191–343 amino acids of the PsnNAC036 was a potent activation domain. Furthermore, overexpression of PsnNAC036 stimulated plant growth and enhanced salinity and HT tolerance. Moreover, 14 stress-related genes upregulated in the transgenic plants under high salt and HT conditions may be potential targets of the PsnNAC036. All the results demonstrate that PsnNAC036 plays an important role in salt and HT stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Functional Characterization of PsnNAC036 under Salinity and High Temperature Stresses

Zhang

Cheng

Yao

et al. 2021

IJMS

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“…For example, the NAC11 gene of Ammopiptanthus mongolicus encodes a transcription factor that can respond to drought, low temperature, high salt and other stresses. The translated protein is localized in the nucleus and plays an important role in drought, cold and salt tolerance ( Xinyue et al, 2019 ). In the NAC family of corn ( Zea mays L.), overexpression of ZmNAC 071 in Arabidopsis enhanced the sensitivity of transgenic plants to ABA and osmotic stress ( Lin et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

Yang

Zhu

Huang

et al. 2021

PeerJ

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Solanum muricatum (Pepino) is an increasingly popular solanaceous crop and is tolerant of drought conditions. In this study, 71 NAC transcription factor family genes of S. muricatum were selected to provide a theoretical basis for subsequent in-depth study of their regulatory roles in the response to biological and abiotic stresses, and were subjected to whole-genome analysis. The NAC sequences obtained by transcriptome sequencing were subjected to bioinformatics prediction and analysis. Three concentration gradient drought stresses were applied to the plants, and the target gene sequences were analyzed by qPCR to determine their expression under drought stress. The results showed that the S. muricatum NAC family contains 71 genes, 47 of which have conserved domains. The protein sequence length, molecular weight, hydrophilicity, aliphatic index and isoelectric point of these transcription factors were predicted and analyzed. Phylogenetic analysis showed that the S. muricatum NAC gene family is divided into seven subfamilies. Some NAC genes of S. muricatum are closely related to the NAC genes of Solanaceae crops such as tomato, pepper and potato. The seedlings of S. muricatum were grown under different gradients of drought stress conditions and qPCR was used to analyze the NAC expression in roots, stems, leaves and flowers. The results showed that 13 genes did not respond to drought stress while 58 NAC genes of S. muricatum that responded to drought stress had obvious tissue expression specificity. The overall expression levels in the root were found to be high. The number of genes at extremely significant expression levels was very large, with significant polarization. Seven NAC genes with significant responses were selected to analyze their expression trend in the different drought stress gradients. It was found that genes with the same expression trend also had the same or part of the same conserved domain. Seven SmNACs that may play an important role in drought stress were selected for NAC amino acid sequence alignment of Solanaceae crops. Four had strong similarity to other Solanaceae NAC amino acid sequences, and SmNAC has high homology with the Solanum pennellii. The NAC transcription factor family genes of S. muricatum showed strong structural conservation. Under drought stress, the expression of NAC transcription factor family genes of S. muricatum changed significantly, which actively responded to and participated in the regulation process of drought stress, thereby laying foundations for subsequent in-depth research of the specific functions of NAC transcription factor family genes of S. muricatum.

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“…The seven soybean NAC genes correspond to four Arabidopsis NAC homologs and are all up-regulated by FeD conditions. Glyma.14G084300 and Glyma.17G240700 (NAC011, At1g32510) enhance tolerance to drought and cold stress [77]. Glyma.02G222300, Glyma.07G048000, and Glyma.16G016700 (NAC9, At4g35580) are associated with osmotic stress signaling and plant immunity [78,79].…”

Section: Gene Expression In Mandarin (Ottawa) Leaves and Rootsmentioning

confidence: 99%

Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

O’Rourke¹,

Morrisey²,

Merry

et al. 2021

IJMS

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The soybean (Glycine max L. merr) genotype Fiskeby III is highly resistant to a multitude of abiotic stresses, including iron deficiency, incurring only mild yield loss during stress conditions. Conversely, Mandarin (Ottawa) is highly susceptible to disease and suffers severe phenotypic damage and yield loss when exposed to abiotic stresses such as iron deficiency, a major challenge to soybean production in the northern Midwestern United States. Using RNA-seq, we characterize the transcriptional response to iron deficiency in both Fiskeby III and Mandarin (Ottawa) to better understand abiotic stress tolerance. Previous work by our group identified a quantitative trait locus (QTL) on chromosome 5 associated with Fiskeby III iron efficiency, indicating Fiskeby III utilizes iron deficiency stress mechanisms not previously characterized in soybean. We targeted 10 of the potential candidate genes in the Williams 82 genome sequence associated with the QTL using virus-induced gene silencing. Coupling virus-induced gene silencing with RNA-seq, we identified a single high priority candidate gene with a significant impact on iron deficiency response pathways. Characterization of the Fiskeby III responses to iron stress and the genes underlying the chromosome 5 QTL provides novel targets for improved abiotic stress tolerance in soybean.

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Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses

Cited by 18 publications

References 53 publications

Functional Characterization of PsnNAC036 under Salinity and High Temperature Stresses

Functional Characterization of PsnNAC036 under Salinity and High Temperature Stresses

Transcriptome-wide and expression analysis of the NAC gene family in pepino (Solanum muricatum) during drought stress

Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

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