Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

O’Rourke, Jamie A.; Morrisey, Michael J.; Merry, Ryan; Espina, Mary Jane; Lorenz, Aaron J.; Stupar, Robert M.; Graham, Michelle A.

doi:10.3390/ijms222011032

Cited by 3 publications

(5 citation statements)

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“…Based on this, Glyma.06G115800 is likely to maintain Al balance by transporting Al into cells or out of cells. Previous research has demonstrated that Glyma.15G251300 encoding nicotianamine synthase NAS1 plays a central role in long-distance Fe transport [ 44 ]. Though Al transport was not involved in the research, other research has proved that mugineic acid (MA), with nicotianamine (NA) as the important intermediate in its production, has the ability to bind to Al [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

GmWRKY81 Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean

Shu

Zhou

Xian

et al. 2022

IJMS

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Aluminum (Al) toxicity is an essential factor that adversely limits soybean (Glycine max (L.) Merr.) growth in acid soils. WRKY transcription factors play important roles in soybean responses to abiotic stresses. Here, GmWRKY81 was screened from genes that were differentially expressed under Al treatment in Al-tolerant soybean Baxi10 and Al-sensitive soybean Bendi2. We found that GmWRKY81 was significantly induced by 20 μM AlCl3 and upregulated by AlCl3 treatment for 2 h. In different tissues, the expression of GmWRKY81 was differentially induced. In 0–1 cm root tips, the expression of GmWRKY81 was induced to the highest level. The overexpression of GmWRKY81 in soybean resulted in higher relative root elongation, root weight, depth, root length, volume, number of root tips and peroxidase activity but lower root average diameter, malonaldehyde and H2O2 contents, indicating enhanced Al tolerance. Moreover, RNA-seq identified 205 upregulated and 108 downregulated genes in GmWRKY81 transgenic lines. Fifteen of these genes that were differentially expressed in both AlCl3-treated and GmWRKY81-overexpressing soybean had the W-box element, which can bind to the upstream-conserved WRKY domain. Overall, the combined functional analysis indicates that GmWRKY81 may improve soybean Al tolerance by regulating downstream genes participating in Al3+ transport, organic acid secretion and antioxidant reactions.

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Section: Discussionmentioning

confidence: 99%

GmWRKY81 Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean

Shu

Zhou

Xian

et al. 2022

IJMS

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“…SPAD measurements have been used to map IDC QTLs and confirm the identification of IDC QTLs visual scores ( Lin et al., 1997 , Lin et al., 2000 ; Assefa et al., 2020 ). In addition, SPAD measurements have been used in IDC gene expression analyses ( O’Rourke et al., 2007 , O’Rourke et al., 2009 ; Atencio et al., 2021 ; Kohlhase et al., 2021 ) and to phenotype VIGS plants in terms of response to iron stress ( Atwood et al., 2014 ; O’Rourke et al., 2021 ). The average of six SPAD readings per trifoliolate, two readings per leaflet, was calculated for the first and second trifoliolates for eight plants per construct.…”

Section: Methodsmentioning

confidence: 99%

“…In soybean, researchers have adopted virus-induced gene silencing (VIGS) as a relatively fast and inexpensive tool that can target single genes or gene families ( Zhang and Ghabrial, 2006 ; Zhang et al., 2013 ). Targeted traits include resistance genes and defense gene networks ( Meyer et al., 2009 ; Liu et al., 2011 ; Pandey et al., 2011 ; Liu et al., 2012 ; Cooper et al., 2013 ; Kandoth et al., 2013 ; Morales et al., 2013 ; Xu et al., 2018 ; Pedley et al., 2019 ) and candidate abiotic stress genes ( Atwood et al., 2014 ; Sun et al., 2016 ; Ogata et al., 2017 ; O’Rourke et al., 2021 ; O’Rourke and Graham, 2022 ). Atwood et al.…”

Section: Introductionmentioning

confidence: 99%

“…RNA-seq of GmRPA3c -silenced plant and empty vector controls revealed that GmRPA3c silencing resulted in massive transcriptional reprogramming of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis, and iron uptake and transport ( Atwood et al., 2014 ). Similarly, O’Rourke et al. (2021) used VIGS to target 10 genes within the IDC QTL on Gm05 identified by Merry et al.…”

Section: Introductionmentioning

confidence: 99%

“…In soybean, researchers have adopted virusinduced gene silencing (VIGS) as a relatively fast and inexpensive tool that can target single genes or gene families (Zhang and Ghabrial, 2006;Zhang et al, 2013). Targeted traits include resistance genes and defense gene networks (Meyer et al, 2009;Liu et al, 2011;Pandey et al, 2011;Liu et al, 2012;Cooper et al, 2013;Kandoth et al, 2013;Morales et al, 2013;Xu et al, 2018;Pedley et al, 2019) and candidate abiotic stress genes (Atwood et al, 2014;Sun et al, 2016;Ogata et al, 2017;O'Rourke et al, 2021;O'Rourke and Graham, 2022). Atwood et al (2014) used VIGS to silence Replication Protein A subunit 3 (GmRPA3c), one of the most significantly differentially expressed genes identified by O'Rourke et al (2009), which is located within an IDC QTL on soybean chromosome Gm20 (Lin et al, 1997, Lin et al, 1998.…”

Section: Introductionmentioning

confidence: 99%

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GmGLU1 and GmRR4 contribute to iron deficiency tolerance in soybean

Kohlhase,

O’Rourke,

Graham

2024

Front. Plant Sci.

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Iron deficiency chlorosis (IDC) is a form of abiotic stress that negatively impacts soybean yield. In a previous study, we demonstrated that the historical IDC quantitative trait locus (QTL) on soybean chromosome Gm03 was composed of four distinct linkage blocks, each containing candidate genes for IDC tolerance. Here, we take advantage of virus-induced gene silencing (VIGS) to validate the function of three high-priority candidate genes, each corresponding to a different linkage block in the Gm03 IDC QTL. We built three single-gene constructs to target GmGLU1 (GLUTAMATE SYNTHASE 1, Glyma.03G128300), GmRR4 (RESPONSE REGULATOR 4, Glyma.03G130000), and GmbHLH38 (beta Helix Loop Helix 38, Glyma.03G130400 and Glyma.03G130600). Given the polygenic nature of the iron stress tolerance trait, we also silenced the genes in combination. We built two constructs targeting GmRR4+GmGLU1 and GmbHLH38+GmGLU1. All constructs were tested on the iron-efficient soybean genotype Clark grown in iron-sufficient conditions. We observed significant decreases in soil plant analysis development (SPAD) measurements using the GmGLU1 construct and both double constructs, with potential additive effects in the GmRR4+GmGLU1 construct. Whole genome expression analyses (RNA-seq) revealed a wide range of affected processes including known iron stress responses, defense and hormone signaling, photosynthesis, and cell wall structure. These findings highlight the importance of GmGLU1 in soybean iron stress responses and provide evidence that IDC is truly a polygenic trait, with multiple genes within the QTL contributing to IDC tolerance. Finally, we conducted BLAST analyses to demonstrate that the Gm03 IDC QTL is syntenic across a broad range of plant species.

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Coupling VIGS with Short- and Long-Term Stress Exposure to Understand the Fiskeby III Iron Deficiency Stress Response

O’Rourke

Graham

2022

IJMS

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Yield loss due to abiotic stress is an increasing problem in agriculture. Soybean is a major crop for the upper Midwestern United States and calcareous soils exacerbate iron deficiency for growers, resulting in substantial yield losses. Fiskeby III is a soybean variety uniquely resistant to a variety of abiotic stresses, including iron deficiency. Previous studies identified a MATE transporter (Glyma.05G001700) associated with iron stress tolerance in Fiskeby III. To understand the function of this gene in the Fiskeby III response to iron deficiency, we coupled its silencing using virus-induced gene silencing with RNAseq analyses at two timepoints. Analyses of these data confirm a role for the MATE transporter in Fiskeby III iron stress responses. Further, they reveal that Fiskeby III induces transcriptional reprogramming within 24 h of iron deficiency stress, confirming that like other soybean varieties, Fiskeby III is able to quickly respond to stress. However, Fiskeby III utilizes novel genes and pathways in its iron deficiency response. Identifying and characterizing these genes and pathways in Fiskeby III provides novel targets for improving abiotic stress tolerance in elite soybean lines.

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Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

Cited by 3 publications

References 127 publications

GmWRKY81 Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean

GmWRKY81 Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean

GmGLU1 and GmRR4 contribute to iron deficiency tolerance in soybean

Coupling VIGS with Short- and Long-Term Stress Exposure to Understand the Fiskeby III Iron Deficiency Stress Response

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