Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks

Loreto, Élgion Lúcio da Silva; Deprá, Maríndia; Diesel, Jose Francisco; Panzera, Yanina; Valente, Vera Lúcia da Silva

doi:10.1590/1678-4685-gmb-2017-0068

Cited by 8 publications

(9 citation statements)

References 52 publications

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“…At least a fraction of non-autonomous elements, that usually exceed in number the autonomous one, could be still mobilized by the in trans action of the wild type transposition machinery expressed from autonomous elements. It is believed that trans-mobilized non-autonomous elements are the principal contributors of the dissemination of cis-acting regulatory sequences throughout the genome, inducing transcriptional network rewiring and the alteration of wild type transcriptional patterns [36].…”

Section: Drosophila Tes: a Brief Overviewmentioning

confidence: 99%

“What You Need, Baby, I Got It”: Transposable Elements as Suppliers of Cis-Operating Sequences in Drosophila

Moschetti

Palazzo

Lorusso

et al. 2020

Biology

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Transposable elements (TEs) are constitutive components of both eukaryotic and prokaryotic genomes. The role of TEs in the evolution of genes and genomes has been widely assessed over the past years in a variety of model and non-model organisms. Drosophila is undoubtedly among the most powerful model organisms used for the purpose of studying the role of transposons and their effects on the stability and evolution of genes and genomes. Besides their most intuitive role as insertional mutagens, TEs can modify the transcriptional pattern of host genes by juxtaposing new cis-regulatory sequences. A key element of TE biology is that they carry transcriptional control elements that fine-tune the transcription of their own genes, but that can also perturb the transcriptional activity of neighboring host genes. From this perspective, the transposition-mediated modulation of gene expression is an important issue for the short-term adaptation of physiological functions to the environmental changes, and for long-term evolutionary changes. Here, we review the current literature concerning the regulatory and structural elements operating in cis provided by TEs in Drosophila. Furthermore, we highlight that, besides their influence on both TEs and host genes expression, they can affect the chromatin structure and epigenetic status as well as both the chromosome's structure and stability. It emerges that Drosophila is a good model organism to study the effect of TE-linked regulatory sequences, and it could help future studies on TE-host interactions in any complex eukaryotic genome.

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Section: Drosophila Tes: a Brief Overviewmentioning

confidence: 99%

“What You Need, Baby, I Got It”: Transposable Elements as Suppliers of Cis-Operating Sequences in Drosophila

Moschetti

Palazzo

Lorusso

et al. 2020

Biology

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“…Recently, there has been a growing interest in studies of the behavior of hobo elements which not only can be massively activated under conditions of classical dysgenic crossing [Hmales (with hobo transposons) × E-females (without hobo)] (Blackman et al, 1987) but they can also be active in nongenetic systems, i.e., under conditions of interlinear (between H-males and H-females) and reciprocal (between H-females and E-males) crosses (Bazin and Higuet, 1996;O'Hare et al, 1998;Zakharenko et al, 2006;Yushkova, 2019). The hobo transposon of D. melanogaster belongs to the Ac elements of the hAT superfamily (Loreto et al, 2018). It is active in the germ line and somatic tissue cells (Calvi and Gelbart, 1994;O'Hare et al, 1998;Zakharenko et al, 2006) and also is sensitive to radiation exposure (Ivashenko and Grishaeva, 2002;Yushkova and Zainullin, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…It is active in the germ line and somatic tissue cells (Calvi and Gelbart, 1994;O'Hare et al, 1998;Zakharenko et al, 2006) and also is sensitive to radiation exposure (Ivashenko and Grishaeva, 2002;Yushkova and Zainullin, 2014). The full-length hobo element (∼ 3 kb) has an open reading frame (ORF) encoding transposase and short terminal inverted repeats (TIRs) 12 bp in size, forms duplications at the insertion site of 8 bp (Loreto et al, 2018). Many studies have shown that Drosophila genomes are loaded the defect or truncated hobo copies (up to 1.5 kb) which have a high similarity to a transcriptionally active canonical hobo transposons (Torres et al, 2006;Ortiz and Loreto, 2008;Deprá et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Yushkova

2020

Front. Genet.

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The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.

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“…The discovery of unexpected high amounts of TEs in the genome of distinct species has pointed out toward functions of TEs on these genomes [1, 2, 3]. In fact, current knowledge indicates that TEs have been shaping the evolution of genomes and host species [4], contributing to the creation of new genes [5, 6] and promoting rearrangements frequently associated with new regulatory networks [7, 8, 9]. Moreover, there is evidence that TEs may assist in the control of embryonic development [9, 10] and genomic plasticity [11].…”

Section: Introductionmentioning

confidence: 99%

Evolutionary history and classification of Micropia retroelements in Drosophilidae species

et al. 2019

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Transposable elements (TEs) have the main role in shaping the evolution of genomes and host species, contributing to the creation of new genes and promoting rearrangements frequently associated with new regulatory networks. Support for these hypotheses frequently results from studies with model species, and Drosophila provides a great model organism to the study of TEs. Micropia belongs to the Ty3/Gypsy group of long terminal repeats (LTR) retroelements and comprises one of the least studied Drosophila transposable elements. In this study, we assessed the evolutionary history of Micropia within Drosophilidae, while trying to assist in the classification of this TE. At first, we performed searches of Micropia presence in the genome of natural populations from several species. Then, based on searches within online genomic databases, we retrieved Micropia-like sequences from the genomes of distinct Drosophilidae species. We expanded the knowledge of Micropia distribution within Drosophila species. The Micropia retroelements we detected consist of an array of divergent sequences, which we subdivided into 20 subfamilies. Even so, a patchy distribution of Micropia sequences within the Drosophilidae phylogeny could be identified, with incongruences between the species phylogeny and the Micropia phylogeny. Comparing the pairwise synonymous distance (dS) values between Micropia and three host nuclear sequences, we found several cases of unexpectedly high levels of similarity between Micropia sequences in divergent species. All these findings provide a hypothesis to the evolution of Micropia within Drosophilidae, which include several events of vertical and horizontal transposon transmission, associated with ancestral polymorphisms and recurrent Micropia sequences diversification.

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Drosophila relics hobo and hobo-MITEs transposons as raw material for new regulatory networks

Cited by 8 publications

References 52 publications

“What You Need, Baby, I Got It”: Transposable Elements as Suppliers of Cis-Operating Sequences in Drosophila

“What You Need, Baby, I Got It”: Transposable Elements as Suppliers of Cis-Operating Sequences in Drosophila

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Evolutionary history and classification of Micropia retroelements in Drosophilidae species

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