Identification and quantification of genipin and geniposide from Genipa americana L. by HPLC-DAD using a fused-core column

Abstract

On this work, it was developed a fast, simple and selective method for quantification of genipin and geniposide from unripe fruits of genipap, which are known as natural colorants, blue and yellow, respectively. The compounds separation was performed in a fused-core C18 column using as mobile phase water (A) and acetonitrile (B) both acidified with 0.1% formic acid, with the following gradient: 0 min, 99% A; 9 min, 75% A; 10 min, 99% A and 13 min, 99% A. The temperature and flow rate that allowed the best chromatographic performance were 35 °C and 1.5 mL/min, respectively, resulting a total run time of 13 min, including column clean-up and re-equilibration. This short analysis time represents an advantage compared to the methods reported in the literature where the running times are 2-5 times greater. The detection wavelength was set at 240 nm. The method validation was performed based on specificity, linearity, detection and quantification limits, precision and accuracy, according to OCH methodology. Finally, the developed method was suitable for monitoring analysis of those compounds content in vegetable samples.

Physicochemical Characterization, Stability and Cytotoxicity of a Blue Dye Obtained from Genipap (Genipa americana L.) Fruit

“…Water has a higher polarity than ethanol, and may justify better extraction of the dye ( 18 ). Genipin is present in the immature genipap fruit and is responsible for the formation of the blue colour through the reaction with amino acids in the presence of oxygen ( 19 ). The extraction performed by Neves et al ( 14 ) resulted in bluish tones, which corroborate the findings of this study.…”

Synergic effect of natural deep eutectic solvent and high-intensity ultrasound on obtaining a ready-to-use genipin extract: Crosslinking and anti-neurodegenerative properties

“…Genipin was separated using a column Poroshell 120, C 18 (4.6 × 100 mm, 2.7 μm) (Agilent Technologies, Santa Clara, CA, United States). Separation was carried out according to Nathia-Neves, Nogueira, Vardanega, and Meireles (2018) , using a mobile phase composed of water (A) and ACN (B), both acidified with 0.1 % formic acid and applying the following gradient: 0 min, 99 % A; 9 min, 75 % A; 10 min, 99 % A and 13 min, 99 % A. Injection volume was 10 µL. The column temperature was set at 35 °C and the flow rate at 1.5 mL/min.…”

In Vitro and In Vivo Antidiabetic Potential of Monoterpenoids: An Update

“…The iridoids genipin [methyl-1-hydroxy-7-(hydrozymethyl)-1,4a,5,7 tetrahydrocyclopenta[c]pyran-4-carboxylate] and geniposide [methyl (1S,4aS,7aS)-7-(hydroxymethyl)-1-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1,4a,5,7a tetrahydrocyclopenta[c]pyran-4-carboxylate] exist in many plants as secondary metabolites. The basic structural skeleton of iridoids is a cyclopentane-[C]-pyran ring fused with a six-membered heterocycle oxygenate [ 185 ]. At C1 position of the pyran ring, the hydroxyl group can be replaced with a sugar moiety to form the genipin glycoside, geniposide.…”