Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera

Abstract

The potential of cantabrian anchovy (Engraulis encrarischolus) viscera as a source of bioactive compounds is of interest for both, pharmaceutical and food industries. Cantabrian anchovy guts and heads were freeze-dried, extracted with methanol and subjected to fractionation by solvent partitioning using hexane, ethyl acetate, and butanol. Fractions were tested for antimutagenic, antioxidant, antifungal, and antibacterial activity using the Ames test; DPPH, ABTS, and FRAP assays; the radial grown inhibition assay; and the microbroth dilution method, respectively. Five fractions were obtained from the anchovy gut methanolic extract, in addition to the hexane-(HF), ethyl acetate-(EAF), and butanol-soluble (BF) fractions, an aqueous-soluble fraction (ALF) and precipitated crystals (ACF) in this were also obtained. HF and EAF resulted to be antimutagenic, HF and ALF showed antifungal activity, BF and ACF showed the highest antioxidant potential, and HF and BF were antibacterial against several strains. Anchovy gut, which to the present study had not been reported for any bioactivity, has antimutagenic, antifungal, antioxidant, and antibacterial compounds, which need to be isolated for full characterization and study.

Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying

“…According to Burgos-Hernández et al [23] and Ramos-Hernández et al [10], the composition of the primary level of the extract, prevents HBVC from fully expressing their biological effects. Hence, a separation into fractions by column chromatography is required.…”

Structural and biological properties of protein hydrolysates from seafood by-products: a review focused on fishery effluents

“…Suárez-Jiménez et al (2015) reported that collagen hydrolysates from jumbo squid by-products exhibited an effective inhibition of the induced mutation with AFB 1 of S. typhimurium TA98 and TA100 strains. Additionally, Burgos-Hernández et al (2016) investigated the antimutagenic potential of several fractions obtained from anchovy viscera against AFB 1 using S. typhimurium TA98 and TA100 tester strains. Dn the other hand, several studies reported the antimutagenic activity of some compounds obtained from seafood products such as white shrimp (López-Saiz et al, 2016), octopus (Cruz-Ramírez et al, 2015), and some other marine resources such as several species of seaweed (Dsuna-Ruiz et al, 2016).…”

Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera

“…Lately, there is a particular interest in researching the antioxidant activity of fish viscera, e.g., Abdelhedi et al (2017) had obtained low molecular weight (MW) peptide (<1 kDa) by the ultrafiltration from smooth hound viscera protein hydrolysates and the peptide exhibited good antioxidant capacity. Burgos-Hernandez et al (2016) investigated bioactive fractions from cantabrian anchovy ( Engraulis encrarischolus ) viscera and the fractions showed the antioxidant activity through various assays. In addition, there were many other studies on fish viscera protein hydrolysates ( Villamil, Vaquiro & Solanilla, 2017 ).…”