Artemisia annua increases resistance to heat and oxidative stresses, but has no effect on lifespan in Caenorhabditis elegans

Abstract

It is suggested that oxidative stress induced by cellular reactive oxygen species is one of the major causal factors of aging. The effect of dietary supplementation of anti-oxidants on response to environmental stressors and lifespan has been studied in various model organisms. In the present study, we examine the effect of Artemisia annua extract on resistance to oxidative, heat, and ultraviolet stresses in the nematode Caenorhabditis elegans. Artemisia annua significantly increases survival under oxidative and heat stresses, however has no effects in response to ultraviolet stress. Then, we measured the in vivo changes in expression of stress-responsive genes by Artemisia annua using green fluorescence protein. The expression of hsp-16.2, known to be involved in response to heat stress, is significantly increased by Artemisia annua supplementation. An anti-oxidant gene, sod-3, was also up-regulated by Artemisia annua. However, both mean and maximum lifespan of Caenorhabditis elegans was not altered by dietary supplementation of Artemisia annua. These findings indicate that Artemisia annua confers health-promoting effects through increasing the resistance to environmental stressors and has no effect on lifespan in C. elegans. Dur study suggests that Artemisia annua can be used for the development of novel natural therapeutics for diseases caused by environmental stressors.Keywords: Artemisia annua; oxidative stress; heat stress; lifespan; C. elegans.Practical Application: This study shows Artemisia annua has an anti-stress activity and induces stress-responsive genes in vivo. These results provide scientific backgrounds necessary for the extension of the therapeutic applications of A. annua.

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“…Among wormwood species, Artemisia annua L. attracts special attention due to its effectiveness against malaria, cancer [1], inflammation [2] and oxidative stresses [3,4]. This plant is rich with terpenoids; phenolic compounds, such as coumarins and flavonoids [5,6]; and lipopolysaccharides [2], and is considered to be the only plant source of artemisininthe main sesquiterpene with a powerful antimalarial effect [7,8].…”

Supplementation with Triptolide Increases Resistance to Environmental Stressors and Lifespan in C. elegans

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