Optimizing functional properties of perilla protein isolate using the response surface methodology

He, Dongliang; Zhang, Zhijun; Li, Huizhen; Xia, Yaoyao; Li, Xiaojun; Chen, Tie

doi:10.1590/1678-457x.00817

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Furthermore, compared with traditional methods, ultrasonic-assisted extraction (UAE) is considered as an effective extraction method that can greatly reduce the use of solvents and extraction time [17]. UAE can increase the extraction yield of proteins from defatted rice bran [18], perilla [19], defatted soy flakes [20], and the sea bass [21]. In general, ultrasound can produce strong shear forces, disrupt the cell structure [22], and increase the mass and heat transfer [23,24], which can enhance the yield of extracted components and the extraction rate.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins

Taha

et al. 2019

Molecules

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The effects of ultrasonic-assisted extraction (UAE, 200 W, 20 min) on the yield and physicochemical properties of different walnut proteins (WNPs, including albumin, globulin, and glutelin) were investigated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that UAE could result in protein molecular fragmentation of albumin, but did not affect the major bands of globulin and glutelin. The CD spectra demonstrated that different WNPs obtained by UAE had different changes in their secondary structure. Under UAE, there was an increase in surface hydrophobicity (H0) of albumin and gluten and no change in the fluorescence intensity, while decreases were observed in the H0 and fluorescence intensity of globulin; and the contents of total and surface free sulfhydryl in albumin dramatically decreased. UAE reduced the size of the particles and the dimension of the microstructures in albumin and gluten, indicating that ultrasound could unfold protein aggregates. In addition, UAE increased the solubility, emulsifying activity (EA), foaming capacity (FC), and foam stability (FS) of the obtained proteins. The above results indicate that ultrasound extraction is a promising approach to improve the extraction yield and properties of walnut proteins.

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Section: Introductionmentioning

confidence: 99%

Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins

Taha

et al. 2019

Molecules

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“…Recently, most researches pay attention to investigate the effects of ultrasonication technology and microwave treatments on the extraction of biologically active substances and natural products due to simple operation, high extraction efficiency, and environmental protection (Munira et al., 2019). Some literatures have reported that ultrasonic, microwave assisted extraction can accelerate the protein dissolution, increase the protein yield from rice bran, perilla, soy flakes, and sweet potato (Habinshuti et al., 2020; He et al., 2018). In addition to the extraction methods, various parameters such as solvent/solid ratio, pH, temperature, ionic strength, solvent type, extraction time, also affect protein extractability (Zhao et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Ultrasound‐microwave assisted extraction of proteins from Moringa oleifera leaves: Comparative optimization study and LC‐MS analysis of the protein concentrate

Cheng

Shu

Chen

et al. 2021

J. Food Process. Preserv.

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Moringa oleifera leaves contain significant amount of high quality protein which can be utilized as nutrition supplements and food functional ingredients. In this study, the extraction process of M. oleifera leaves protein was optimized by response surface methodology. Traditional solvent extraction conditions for maximum protein recovery were 0.15‐M Tris‐HCl concentration, 38‐min extraction time, and 128:1 (v/w) solvent/solid ratio at 34°C, and the protein yield was 68.99 mg/g. Furthermore, ultrasound‐microwave assisted extraction (UMAE) was adopted to product the leaves protein, and the results showed that the optimum extraction conditions were solvent/solid ratio 91:1 (v/w), extraction time 148 s, extraction temperature 41°C, microwave power 81 W, the yield of protein was 82.07 mg/g. Compared with the conventional solvent extraction method, UMAE exhibited significantly high extraction efficiency. Through LC‐MS technology identification and bioinformatics analysis, it is verified that the extracted proteins could provide energy and material basis for the functions of M. oleifera leaf. Practical applications Moringa oleifera leaves have a high protein content and reasonable composition of essential amino acids, which is a high‐quality plant protein with great development potential. In the present study, the response surface method was used to optimize the process of ultrasonic and microwave synergistic extraction of M. oleifera leaves protein, which is effective and significantly increased the protein yield. According to the results of LC‐MS identification and bioinformatics analysis, the proteins played a leading role in maintaining the normal tissue, structure, and function of M. oleifera leaves, participated in the growth and metabolism of M. oleifera leaves, and provided material and energy supply for the functional performance of M. oleifera leaves.

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Polypeptides from traditional Chinese medicine: Comprehensive review of perspective towards cancer management

Jia,

Zhu,

Zhou

et al. 2024

International Journal of Biological Macromolecules

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Optimizing functional properties of perilla protein isolate using the response surface methodology

Abstract: (Dnline) 2 Materials and Methods 2.1 Sample preparation and chemicals Fresh whole perilla seeds were collected in Dctober 2015

Cited by 6 publications

References 28 publications

Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins

Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins

Ultrasound‐microwave assisted extraction of proteins from Moringa oleifera leaves: Comparative optimization study and LC‐MS analysis of the protein concentrate

Polypeptides from traditional Chinese medicine: Comprehensive review of perspective towards cancer management

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