Salt stress alleviation by seed priming with silicon in lettuce seedlings: an approach based on enhancing antioxidant responses

Abstract

Seed germination and seedling growth are the stages most sensitive to salt stress, which can induce ion-specific effects and oxidative stress. Although silicon (Si) has been known to improve plant defense systems against stressful-conditions, little is known about seed priming with Si and mechanisms of Si-mediated alleviation of salt stress in seedlings. To further the modulation of salt-stress responses and their relation with Si, seed priming with calcium silicate (0, 0.05 and 0.1 mM Ca 2 SiO 4) was used in lettuce seeds to improve seed germination and tolerance on subsequent salt-stress exposure (0 and 50 mM NaCl). The effect of Si-priming was assessed on germination, germination rate index and mean germination time and survival of seedlings in salt-stressful conditions supported by biochemical approach involving antioxidant responses. Overall results indicated that Si plays a role in alleviating the negative effects of salt stress by improving germination performance and displayed increased SOD, CAT and GR activities and reduced malondialdehyde and H 2 O 2 contents. Thus, our findings demonstrate that seed-priming with Si is an efficient management technique that can be used to alleviate deleterious effects of salt-stressful on germination of lettuce seeds and enhance salt tolerance of seedlings due the increased activity of ROS-scavenging enzymes.

CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance

“…ROS generation under environmental stress conditions exceeds the scavenging capacity of cellular redox potential, resulting in oxidative stress. [ 39,51–53 ] ROS over‐production poses a significant threat to cells via enzymes inhibition, lipids peroxidation, proteins oxidation, damaging nucleic acids, and initiation of programmed cell death (PCD pathway), and consequently leads to cell death. [ 54–56 ] Thus, plant ability to maintain ROS homeostasis is associated with its stress tolerance.…”

Nanoceria seed priming enhanced salt tolerance in rapeseed through modulating ROS homeostasis and α-amylase activities

“…In wheat, seed priming with silver nanoparticles (AgNPs) improved the germination and growth parameters by regulating hormonal balance and photosynthetic efficiency under salt stress [ 44 ]. Under salt stress, nanopriming with calcium silicate (Ca 2 SiO 4 ) in lettuce improved seed germination through triggering antioxidant enzymes including SOD, CAT and GR (glutathione reductase) to effectively scavenge the over-produced ROS [ 45 ]. Water soluble carbon nanoparticles (CNPs) promoted lettuce germination and lateral root growth under salt stress [ 43 ].…”

Tuz Stresi Koşullarında Havuçta (Daucus carota L.) Eksojen Silikon Dioksit Uygulamaları

“…The applied SiO2 had a stimulating effect on germination by regulating antioxidant enzyme activities, total soluble sugar and proline content (Ali et al, 2021). Similarly, it was stated that the application of SiO2 in tomato (Shi et al, 2014) and lettuce (Alves et al, 2020) improved germination depending on the antioxidant enzyme activity. Supporting the results of this study, it was determined that SiO2 applications promoted germination in wheat (Feghhenabi et al, 2020), maize (Khan et al, 2015) and cucumber (Gou et al, 2020) under salt stress conditions.…”