Contribution of non-target-site resistance in imidazolinone-resistant Imisun sunflower

Breccia, Gabriela; Gil, Mercedes; Vega, Tatiana Alejandra; Altieri, Emiliano; Bulos, Mariano; Picardi, Liliana Amelia; Nestares, G.

doi:10.1590/1678-4499.2016.336

Cited by 12 publications

(26 citation statements)

References 25 publications

(27 reference statements)

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“…Ahas1 is a multiallelic locus and the only member of this gene family where all the induced and natural mutations for herbicide resistance in sunflower have been described (Kolkman et al, 2004;. Our group findings revealed that the mechanism of resistance endowed by modifier gene Imr2 is related to non-target-site resistance (Breccia et al, 2017). Our group findings revealed that the mechanism of resistance endowed by modifier gene Imr2 is related to non-target-site resistance (Breccia et al, 2017).…”

Section: Partial Male Sterility In Imisun Sunflower: Imazapyr Treatmementioning

confidence: 63%

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Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters ahas Gene Expression

et al. 2018

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Imidazolinones are powerful herbicides that inhibit branched‐chain amino acid biosynthesis by targeting the catalytic subunit of acetohydroxyacid synthase (AHAS). Imidazolinone application in the advanced vegetative or early reproductive developmental stages is associated with male sterility in resistant sunflower (Helianthus annuus L.); however, the underlying mechanism of this sterility remains unknown. This study describes the morphological, cytoembryological, and molecular alterations induced by imazapyr (IM) treatment on reproductive tissues at different developmental stages in two sunflower genotypes, resistant and intermediate resistant, respectively. Pollen and seed physiological variables were compared between the treated and control plants. The number of pollen grains per flower and viable seeds were negatively affected by IM treatment in the intermediate‐resistant genotype, and the biometric traits of early developed disc flower were also significantly different in this genotype. Differential interference contrast microscopy revealed that IM treatment slightly accelerates megagamethophyte development. Anther observations at microsporogenesis using confocal microscopy show that the sporogenous tissue was damaged. Furthermore, the expression profiles of the sunflower AHAS paralogs (ahas1, ahas2, and ahas3) were measured by quantitative polymerase chain reaction in the anthers and pistils of two developmental stages in treated and control plants. Imazapyr treatment in early reproductive growth stages clearly induces divergent expression patterns in the ahas gene family. These findings provide new insight into a novel chemical method for inducing male sterility in sunflowers and enhance our understanding of the effects of AHAS‐inhibitor herbicides in reproductive tissues.

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Section: Partial Male Sterility In Imisun Sunflower: Imazapyr Treatmementioning

confidence: 63%

“…The Imr2 locus has been related to a non-target-site resistance mechanism associated with P450s isozymes (Breccia et al, 2017). The effective herbicide action probably differed because R and I genotypes differ in their herbicide resistance level.…”

Section: Discussionmentioning

confidence: 99%

Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters ahas Gene Expression

et al. 2018

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“…The major semidominant gene Imr1 is an allelic variant of the ahasl1 locus that codes for the acetohydroxyacid synthase (AHAS) catalytic subunit, which is the target of imidazolinones (Kolkman et al, 2004). The identity of the modifier gene Imr2 remains unknown, but it could be related to metabolism of xenobiotics (Breccia et al, 2017). Therefore, resistance in Imisun sunflower appears as a combination of both…”

Section: Researchmentioning

confidence: 99%

“…Afterward, the activated xenobiotic is conjugated using thiols or sugars and actively transported into the vacuole or extracellular space, where it is finally degraded (Yuan et al, 2007). Few studies have described the participation of these gene families in metabolizing herbicides in the Helianthus genera, and they were particularly focused on P450s (Didierjean et al, 2002;Kaspar et al, 2011;Breccia et al, 2017) and GSTs (Balabanova et al, 2017). Few studies have described the participation of these gene families in metabolizing herbicides in the Helianthus genera, and they were particularly focused on P450s (Didierjean et al, 2002;Kaspar et al, 2011;Breccia et al, 2017) and GSTs (Balabanova et al, 2017).…”

Section: Transcript Profiling Of Non-target-site Imidazolinone Resistmentioning

confidence: 99%

Transcript Profiling of Non‐Target‐Site Imidazolinone Resistance in Imisun Sunflower

et al. 2018

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Imidazolinone resistance found in a wild sunflower (Helianthus annuus L.) population was successfully transferred to a cultivated inbred line developing ‘Imisun’ sunflowers. Genetic regulation of this trait has been reported to involve two genes: Imr1, an allelic variant of ahasl1 locus that codes for acetohydroxyacid synthase catalytic subunit, and the modifier Imr2, whose identity remains unknown, but it could be related to non‐target‐site resistance such as xenobiotic metabolism. The aim of the present study was to characterize the gene expression of resistant and susceptible sunflower lines in response to imazethapyr herbicide by complementary DNA amplified fragment‐length polymorphism (cDNA‐AFLP). Three assays were performed to determine (i) optimal herbicide treatment concentration, (ii) duration of herbicide treatment, and (iii) in vitro acetohydroxyacid synthase activity to assess enzyme inhibition levels. An important number of genes related to metabolism of xenobiotics and stress was found: cytochrome P450 monooxygenases, UDP‐glucuronosyl/UDP‐glucosyltransferases, glycosyltransferases, and ATP‐binding cassette transporters, among others. These results suggest that non‐target‐site resistance mechanisms may contribute to herbicide resistance in Imisun sunflower and could be related to the modifier gene Imr2. Using cDNA‐AFLP, we were able to detect candidate detoxification‐related genes potentially involved in imidazolinone resistance in sunflower.

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“…The inheritance of Imisun is additively controlled by two genes, one of them is Ahasl1‐1 and the other a modifier or enhancer factor . The modifier gene is involved in the non‐target‐site component of resistance of this trait . Ahasl1‐4 allele endows high levels of resistance to at least four of the five families of herbicides targeting AHAS but this trait is not commercially available yet …”

Section: Introductionmentioning

confidence: 99%

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower

Breccia

Gianotto

Altieri³

et al. 2018

Pest Management Science

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Contribution of non-target-site resistance in imidazolinone-resistant Imisun sunflower

Cited by 12 publications

References 25 publications

Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters ahas Gene Expression

Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters ahas Gene Expression

Transcript Profiling of Non‐Target‐Site Imidazolinone Resistance in Imisun Sunflower

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower

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