Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters <i>ahas</i> Gene Expression

Ochogavía, Ana Claudia; Novello, María Alejandra; Bianchi, Marta B.; Picardi, Liliana Amelia; Nestares, G.

doi:10.2135/cropsci2017.12.0726

Cited by 5 publications

(22 citation statements)

References 40 publications

(67 reference statements)

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“…The number of pollen grains per flower was counted with a hemocytometer on five closed flowers (E1 phenophase). A pollen grains suspension was made and analyzed under stereomicroscope as described by Ochogavía et al (2018).…”

Section: Number Of Pollen Grains Per Flowermentioning

confidence: 99%

“…Pollen grains were analyzed by differential interference contrast (DIC) microscopy as described by Ochogavía et al (2018). Anthers of five flowers of three different treated plants (i.e., IM-1X, IM-2X, and the control of each genotype) were clarified using the Herr's solution (Herr Jr., 1971).…”

Section: Pollen Grain Diameter Determinationmentioning

confidence: 99%

“…Fresh pollen was collected during E3 phenophase (anther dehiscence period (Schneiter and Miller 1981) and treated with lactophenol and 0.01% aniline blue (W/V) (Maneval 1936). The percentage of viable pollen grains was estimated as described by Ochogavía et al 2018).…”

Section: Pollen Viabilitymentioning

confidence: 99%

“…In sunflower, described IM-induced male sterility during the early reproductive development stages in imidazolinone resistant lines. Recently, a study by Ochogavía et al (2018) have provided evidence of morphological, cytoembryological, and molecular alterations in the reproductive tissue in 'Imisun' sunflower lines. It was demonstrated that the field recommended dose of imazapyr affects the female and male reproductive development, reducing the pollen grain number and finally the seed production.…”

Section: Introductionmentioning

confidence: 99%

“…It was demonstrated that the field recommended dose of imazapyr affects the female and male reproductive development, reducing the pollen grain number and finally the seed production. The treatment also alters the expression patterns of the ahas anther genes during early reproductive stages in sunflower (Ochogavía et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Evidence of accelerated and altered pollen development after Imazapyr treatment in resistant sunflower

et al. 2020

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Imazapyr (IM) treatment applied at early reproductive developmental stages have been associated with induced male sterility in sunflower (Helianthus annuus L.). The aim of this work was to determine the effects induced by two different doses of IM-treatment (1X and 2X; 80 and 160 g a.i.•ha -1 ) on pollen grain yield, pollen viability and on the exine surface, in an intermediate resistant (I) and a completely resistant (R) genotype. An anatomical analysis of some stages during the microsporogenesis and microgametogenesis was also achieved by confocal microscopy. Plants of both genotypes survived the herbicide treatments and showed no external injury symptoms. Differential response to different doses of IM was obtained in R and I genotypes.The pollen grains per flower and the viability in seeds were significantly reduced in the I genotype by the 2X treatment; however, the pollen viability of the remaining grains was not altered, and the diameter of the pollen grains was increased by the herbicide treatment. Anatomical and cytological observations of the sporogenous tissue development were made via confocal microscopy. IM treatment induced alterations in sporogenous cells during the initial phases of microsporogenesis in both genotypes, and the 2X dose accelerated the microgametogenesis process, particularly in the I genotype. These findings improved the understanding of the mechanism underlying the gametocide effects of the imidazolinones in sunflower.

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Section: Number Of Pollen Grains Per Flowermentioning

confidence: 99%

Section: Pollen Grain Diameter Determinationmentioning

confidence: 99%

Section: Pollen Viabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evidence of accelerated and altered pollen development after Imazapyr treatment in resistant sunflower

et al. 2020

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An integrated approach for the characterization of one‐ and two‐gene imazamox‐resistant wheat lines

et al. 2020

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In wheat (Triticum aestivum L.), cultivars resistant to imidazolinone herbicides are an important tool for weed management. The target site of imidazolinones is acetohydroxyacid synthase (AHAS), an enzyme coded by three homeologous genes (ahasL‐A1, ahasL‐B1, and ahasL‐D1). This study aimed to evaluate the effect of the imidazolinone herbicide imazamox on a single‐gene (AhasL‐D1) resistant line (R1) and a two‐gene (AhasL‐B1 and AhasL‐D1) resistant line (R2) at multiple biological levels. These lines showed variation in the transcriptional levels of ahasL homeologs, but no differences in total AHAS activity. The relative contribution of resistant alleles to the ahasL transcript pool accounted for 47% in the R1 line and 77% in the R2 line. The in vitro AHAS inhibition of the R1 line was intermediate between the R2 and the susceptible lines. At an anatomical level, high imazamox concentrations induced alterations in the root tip features of R1 plants but did not affect R2 plants, whereas at in vivo AHAS activity and whole‐plant levels, both resistant lines showed high resistance. The herbicide dose that reduced shoot biomass of resistant lines by 50% was 35‐fold higher than that of the susceptible line. We conclude that homeolog variation allows a higher contribution of resistance genes to the ahasL transcript pool in the R1 and R2 lines. Although both resistant lines showed high whole‐plant resistance, the differential root damage induced by imazamox between wheat lines suggests that two‐gene resistant cultivars could be an effective option to avoid the negative impacts caused by herbicide residues in the soil.

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Gene expression profiling by cDNA‐AFLP reveals potential candidate genes for imazapyr‐induced male sterility in sunflower

et al. 2021

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Chemically induced male sterility (CIMS) is a valuable tool for hybrid breeding in crops. In this context, the acetohydroxyacid synthase (AHAS)‐inhibiting imazapyr has been proposed to induce male sterility in Imisun sunflower (Helianthus annuus L.) when applied during early reproductive development in a two‐fold higher than recommended field dose. This study aimed to perform a comparative transcriptomic analysis to find out the molecular bases of the gametocidal effect of imazapyr on two Imisun genotypes differing in their herbicide resistance level: completely resistant and intermediate resistant. The CIMS of treated plants was confirmed at physiological and anatomical levels. A total of 948 differentially expressed transcript‐derived fragments (TDFs) were identified by complementary DNA amplified fragment‐length polymorphism (cDNA‐AFLP) analysis at the three most informative developmental stages of anther development. Several genes related to xenobiotic metabolism and stress, including cytochrome P450 monooxygenases and ATP‐binding cassette transporters, were identified in the completely resistant genotype. Interestingly, a group of genes, including protein kinases, sulfonylurea receptor 1, aspartic signal peptide‐peptidase, and specific membrane transporters, were recognized from the differential TDFs simultaneously detected in both genotypes. Some of these transcripts were validated by quantitative real‐time polymerase chain reaction (qPCR) in both genotypes. Therefore, these transcripts could be exclusively related to the CIMS in sunflower. Based on the results, we suggest a probable model of action for the gametocidal effect of imazapyr. Our research provides new insights into the molecular mechanisms of male sterility induction in sunflower.

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Partial Male Sterility in Imisun Sunflower: Imazapyr Treatment in Advanced Vegetative Stages Decreases Pollen Yield and Alters ahas Gene Expression

Cited by 5 publications

References 40 publications

Evidence of accelerated and altered pollen development after Imazapyr treatment in resistant sunflower

Evidence of accelerated and altered pollen development after Imazapyr treatment in resistant sunflower

An integrated approach for the characterization of one‐ and two‐gene imazamox‐resistant wheat lines

Gene expression profiling by cDNA‐AFLP reveals potential candidate genes for imazapyr‐induced male sterility in sunflower

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