Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding

Miah, Gous; Rafii, Mohd Y.; Ismail, Mohd Razi; Puteh, Adam; Harun, Abdul Rahim; Ashkani, Sadegh; Latif, Abdul

doi:10.1590/1678-4499.0311

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Phenotyping for cultivated rice varieties was not conducted because the phenotypes of the control and some selected varieties for the targeted traits had been reported in earlier studies [ 19 , 20 , 26 , 27 ]. Therefore, only the weedy rice evaluated in this study was phenotyped.…”

Section: Methodsmentioning

confidence: 99%

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Hanafiah

Cheng

Lim

et al. 2022

Life

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While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa) is limited. In this work, we report six novel multiplex assays that use a universal protocol to detect major rice grain quality (amylose content and fragrance) and biotic stress (blast, sheath blight, and bacterial leaf blight) traits with amplified products consisting of up to four primer pairs that can be analyzed using a standard agarose-based gel electrophoresis system. Recent studies have suggested that weedy rice has novel sources of disease resistance. However, an intensive screening of weedy biotypes has not been reported in Malaysia. Accordingly, we employed one of the developed multiplex assays to screen reported genes or quantitative trait loci (QTLs) associated with blast, sheath blight, and bacterial leaf blight diseases in 100 weedy rice biotypes collected from five local fields, with phenotyping performed to validate the genotyping results. In conclusion, our universal multiplex protocol is effective for the large-scale genotyping of rice genetic resources, and it can be employed in routine molecular laboratories with limited resources.

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Section: Methodsmentioning

confidence: 99%

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Hanafiah

Cheng

Lim

et al. 2022

Life

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“…Local varieties that are resistant to the pathogen are the sources of introgression of new resistance genes into some designated rice varieties in a breeding program. Although many researchers pointed out to varieties of rice like IR36, IR64, Moroberekan, OrysicaL lanos5, CO39, Digu, Tetep, Suweon 365, Pongsu Seribu 1, sonarbangla1, and Pongsu Seribu 2 which are found to be resistance to blast attack [53][54][55][56][57]. These varieties are not readily available to rice growers in developing and under-developed parts of the world.…”

Section: The Use Of Resistance Varietiesmentioning

confidence: 99%

Rice Blast Disease (Magnaporthe oryzae): A Menace to Rice Production and Humanity

Agbowuro

Afolabi

Olamiriki

et al. 2020

IJPR

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Rice blast disease is one of the major constraints to rice production, threatening food security globally. Rice grain production losses due to the disease leads economic losses to the farmers, and to an increase in global rice price as a result of the supply that is far below the consumer demand. The losses from the disease annually was estimated to feed over 60 million individual. The disease has been studied comprehensively by researchers due to the importance attached to rice and its vast spread and destructiveness across the globe. A good understanding of the pathogen causing the disease, its life cycle and development, epidemiology, symptoms, management strategy will offer a good insight into the disease incidence and give an appropriate and effective decision-making in its management. Different control measures have been adopted managing the disease, including the use of resistant varieties. Integrated disease management strategies coupled with good agronomy practices are required for successful control of rice blast for food security. This review, therefore, examined the fundamentals of rice blast disease (Magnaporthe oryzae) and offered strategies to minimize the disease activities to ensure proper production and increase the supply of rice grains.

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“…Following that, the Malaysian government has further targeted an increase in the average paddy yield from 4.5 t ha −1 to 6.0 t ha −1 [ 3 ]. Recently, a new variety of rice, currently called PadiU Putra, was developed by [ 4 , 5 ], in which resistance genes from the rice variety Pongsu Seribu 2 were pyramided into MR219. This new variety is claimed to be a blast-resistant rice variety with high yield potential and is to be released for commercial cultivation [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Silicon Improves Yield Performance by Enhancement in Physiological Responses, Crop Imagery, and Leaf and Culm Sheath Morphology in New Rice Line, PadiU Putra

Berahim

Omar

Zakaria

et al. 2021

BioMed Research International

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The PadiU Putra rice line is a blast-resistant and high-yield rice line with high potential. The application of topdressing and the foliar applied method of silicon (Si) treatments could strengthen the culm to resist breakage and ultimately increase yield production. Treatments which consisted of a control, a Si topdressing, and a Si foliar applied were arranged in a randomised complete block design. At 55 days after transplanting (DAT), the foliar applied Si treatments had 59% higher dry matter partitioning to the roots. Meanwhile, at 75 DAT, both Si foliar applied and topdressing method showed increased assimilate partitioning into the culm sheath by 29% and 49%, respectively. Dark green and light yellowish colours were obtained in both Si treatments using UAV, indicating similar results to physiological responses. Remarkably, Si foliar applied treatments enhanced the diameter and width of the outer and inner layers of the diameter of vascular bundles at 75 DAT by 58, 181, and 80%, respectively. The yield production of rice increased by 53% in the Si foliar applied, compared to the control, and produced a 1.63 benefit-cost ratio.

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Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding

Cited by 6 publications

References 29 publications

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Rice Blast Disease (Magnaporthe oryzae): A Menace to Rice Production and Humanity

Silicon Improves Yield Performance by Enhancement in Physiological Responses, Crop Imagery, and Leaf and Culm Sheath Morphology in New Rice Line, PadiU Putra

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