Biphasic Liquid-Liquid Extraction of Biosurfactant from Lactobacillus delbrueckii

Abstract

The present work is focused mainly on optimizing the extraction of glycoprotein-biosurfactant (BS) produced by Lactobacillus delbrueckii in a biphasic liquid-liquid extraction. The production yield of BS is significantly affected by extraction strategies instead of the whole fermentation process, so it becomes a straightforward approach to enhance BS yield while optimizing various extraction parameters. The tailoring of process parameters for BS extraction was achieved by OFAT (one factor at a time) strategy and partition coefficient (Kd) served as the calculation factor for extraction. The optimal yield of BS (5+0.1 g/L) from CFB (from cell-free broth) was achieved by solvents; chloroform, methanol, and, butanol, 1:2:1 (v/v), from cell-free broth (CFB), 30% (v/v), at pH 3.5, temperature 37°C after extraction time of 60 min. Under optimized conditions, the extraction yield was 78.5% higher and subsequently, a co-current, and counter-current system enhanced the extraction yield by 16% (5.8g/L) and 20% (6.0g/L) respectively. The purity of extracted BS (EBS) was confirmed by UV/Visible spectroscopy and HPLC (High-performance liquid chromatography). The concentration-dependent activity profile of EBS analyzed by ODA (oil displacement area; 50.24+0.3 cm 2 ), DCD (drop collapse diameter; 1.1+0.3 cm), and EI24 (73+0.3%), exhibited enhancement by 60, 52, and 55% respectively as compared to control. Thin-layer chromatography (TLC), FTIR (Fourier Transform Infra-Red) and NMR (Nuclear Magnetic Resonance) techniques confirmed the polymeric glycoprotein (65:35 protein: carbohydrates %) nature of BS.

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii : Design expert laden approach

“…The BS was lyophilized (0.12 mbar, 80°C) and stored at 4°C. Furthermore, structural characterization by different techniques such as FTIR, NMR, and TLC evaluated BS as a glycoprotein reported in prior study (Goyal & Singh, 2022).…”

“…The culture broth was centrifuged (10,000 rpm for 20 min at 4°C) to separate the solid biomass. The BS in cell-free broth was solvent extracted as described by previous lab research (Goyal & Singh, 2022). Briefly, solvent was evaporated by rotary evaporator (Rotary Evaporator, Popular India Ltd.) at 50°C.…”

“…Solvent extraction and characterization of BS from L. delbrueckii were conducted following the procedure as illustrated in Figure 8 and evaluated as a glycoprotein with 30:70 (carbohydrate: protein) ratio as described in previous lab research (Goyal & Singh, 2022).…”

Evaluation of the antimicrobial effects of Capsicum, Nigella sativa, Musa paradisiaca L., and Citrus limetta: A review

“…In liquid-liquid extraction separation of required material from liquid solution is made through contact with another insoluble liquid. The required material is separated based on its solubility in different liquids (Chen et al, 2017;El Blidi et al, 2019;Goyal and Singh, 2022).…”

Techno-economics analysis of biosurfactants productions from pseudomonas aeruginosa OPEFB-based with variations in purifications method

“…Until now, oil palm empty fruit bunches have not been used optimally. So far, OPEFB has only been used as raw Generally, there are three main sections of biosurfactant production including pretreatment, fermentation, and purification process [5]. The high demand for biosurfactant production and high production costs have caused many studies to focus on the optimization of production design, extraction process, purification, and characterization [6].…”