Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Bano, Aneela Shah; Khattak, Abdul Mateen; Basıt, Abdul; Alam, Muhd Nazrul Hisham Zainal; Shah, Syed Tanveer; Ahmad, Naveed; Gilani, Syed Abdul Qadir; Ullah, Izhar; Anwar, Sumera; Mohamed, Heba I.

doi:10.1590/1678-4324-2022210200

Cited by 12 publications

(2 citation statements)

References 67 publications

(84 reference statements)

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“…reported that 1 mg/l IBA without BAP had the highest rate of organogenesis.In the second experiment, the high frequency of callogenesis was achieved in a high auxin media which agreed with the ndings of the rst experiment. TheBano et al (2022) showed that the combined 2,4-D and BAP indicated the highest callus weight in 1 mg/l 2,4-D + 1 mg/l BAP medium.. Besides, the result of the second experiment indicated that sometimes calli generated adventitious shoots as well.…”

mentioning

confidence: 90%

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Ouzhand

Mohayeji

Pourseyedi

et al. 2023

Preprint

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Black Zira (Bunium persicum) is an important endemic medicinal plant in Iran. This research was conducted out to assay breaking seed dormancy, develop new protocols for callus induction, somatic embryogenesis, and artificial seed. Seeds were treated with ultrasonic waves in different periods (0, 1, 3, 5, 7, and 9 min). Then, two experiments with IBA (0, 1, 2, 3, and 4 mg L− 1) and a combination of different levels of 2,4-D (0, 1, 2, and 4 mg/l) and BAP (0, 0.5, 1, and 2 mg/l) were designed. Different concentrations of sodium alginate (2, 3, and 4%) and CaCl2 (50, 75, and 100 mM) were prepared for artificial seed production. According to the results, the 7 min ultrasonic waves had the maximum effect on germination (81.87%), while the highest negative effect on germination (2.5%) was reported from 9 min exposure time. In the IBA experiment, the highest percentages of callogenesis, callus weight, and adventitious root were obtained from MS medium containing 4 mg/l IBA. Moreover, 1 mg/l IBA resulted in the highest embryogenesis percentage. In the second experiment, the highest percentage of callus formation and callus weight were achieved in 1 mg/l BAP + 4 mg/l 2,4-D, 0.5 mg/l BAP + 4 mg/l 2,4-D, and 2 mg/l 2,4-D media. The maximum embryogenesis percentage was seen in 0.5 mg/l BAP + 1 mg/l 2,4-D, 0.5 mg/l BAP + 2mg/l 2,4-D, and 1 mg/l 2,4-D media. The highest germination rate (77.77%) was obtained from encapsulated embryos with 3% sodium alginate and 100 mM CaCl2.

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mentioning

confidence: 90%

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Ouzhand

Mohayeji

Pourseyedi

et al. 2023

Preprint

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“…Dua jenis ZPT yang sangat penting dalam memacu pertumbuhan tanaman BAP dan NAA untuk Media Perbanyakan Nanas Toboali adalah sitokinin dan auksin (Dhurve et al 2021;Roussos et al 2021;Shekhawat et al 2021). Golongan sitokinin yang sering digunakan untuk multiplikasi tunas secara in vitro adalah BAP (Benzyl Amino Purine) dan golongan auksin adalah NAA (Naphthalene Acetic Acid) (Ming et al 2019;Batti et al 2020;Choudhary et al 2020;Camalle et al 2021;Insuan et al 2021;Ramabulana et al 2021;Bano et al 2022).…”

Section: Pendahuluanunclassified

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Dahniar

Elvavina

2022

Agrotechnology Res J

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Toboali pineapple variety is a smooth cayenne variety because it contains high antioxidants in the form of vitamins A and C. In addition, it is a source of citric and malic acids, substances that can be used to enhance the taste of the fruit. Pineapple is a plant that is propagated with vegetative organs such as suckers, slips, and crowns. However, conventional propagation has a low propagation rate. Tissue culture is an in vitro technique used in the rapid propagation of plants and in large quantities to meet the needs of cultivation and research. The selection of regeneration media is very influential on the development of plants in tissue culture. This study aimed to obtain the optimal media combination with BAP and NAA in the regeneration of Smooth Cayenne pineapple (Toboali) in vitro for mass propagation of seedlings and genetic engineering research. This study used 6 levels of BAP, namely 0, 1, 2, 3, 4, 5 mg.L-1 and 4 levels of NAA, namely 0, 1, 2, 3 mg.L-1. The treatment used was a combination of BAP and NAA as many as 24 treatments with 3 replications. The best pineapple regeneration medium for mass plant propagation in this study was BAP 3 mg.L-1 without NAA media which produced the highest shoots of 17.33 shoots per explant at 12 week after planting. The best pineapple regeneration medium for genetic engineering research was a combination BAP 3 mg.L-1 and NAA 2 mg.L-1 media which produced 18.33 shoots per explant from embryogenic callus.

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Exploring Melatonin’s Potential as an Alternative Strategy for Protecting Plants from Biotic Stresses

Bhoi,

Samal,

Mahanta

et al. 2023

Melatonin in Plants: A Pleiotropic Molecule for Abiotic Stresses and Pathogen Infection

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Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin

Cited by 12 publications

References 67 publications

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Exploring Melatonin’s Potential as an Alternative Strategy for Protecting Plants from Biotic Stresses

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