Limonium duriusculum (de Girard) Kuntze Exhibits Anti-inflammatory Effect Via NF-κB Pathway Modulation

Hamadou, Meriem Hadjer; Kerkatou, Messaoud; Zucal, Chiara; Bisio, Alessandra; Provenzani, Alessandro; Inga, Alberto; Mustafa, Ahmed M.; Benayache, Fadila; Ameddah, Souad

doi:10.1590/1678-4324-2021200179

Cited by 3 publications

(5 citation statements)

References 51 publications

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“…As a nuclear transcriptional factor, NF-κB regulates the expression of various inflammation and immune response-related genes [ 32 ]. It can be activated in LPS-stimulated macrophages, followed by the phosphorylation of the repressor protein IκBα and dissociated from NF-κB, leading to NF-κB translocation into the nucleus to induce the expression of inflammatory cytokines genes [ 33 ]. In order to clarify whether LGSP and CO could regulate NF-κB translocation, the nuclear and cytoplasmic fractions were determined by Western blotting analysis.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Anti-Inflammatory Effects of Lipophilic Grape Seed Proanthocyanidin and Camellia Oil Combination in LPS-Stimulated RAW264.7 Cells

Zhang

Chen²,

Liang

et al. 2022

Antioxidants

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Combination drug therapy has become an effective strategy to control inflammation. Lipophilic grape seed proanthocyanidin (LGSP) and camellia oil (CO) have been independently investigated to show anti-inflammatory effects, but their synergistic anti-inflammatory effects are unknown. The aim of this study was to investigate the synergistic anti-inflammatory effects of LGSP and CO. The anti-inflammatory activity of LGSP and CO individual or in combination on RAW264.7 cells was detected by MTT assay, Griess reagent, RT-PCR, 2′,7′-dichlorfluoroescein diacetate and Western blot analysis. The combined treatment of LGSP with CO (20 μg/mL and 1 mg/mL) synergistically suppressed the production of NO, TNF-α, IL-6 and ROS. Further studies showed that the synergistic effect was attributed to their suppression of the activation of NF-κB and MAPK signaling pathways. Overall, our findings demonstrate the potential synergistic effect between LGSP and CO in LPS-induced inflammation.

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Section: Resultsmentioning

confidence: 99%

Synergistic Anti-Inflammatory Effects of Lipophilic Grape Seed Proanthocyanidin and Camellia Oil Combination in LPS-Stimulated RAW264.7 Cells

Zhang

Chen²,

Liang

et al. 2022

Antioxidants

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“…In similar studies of the immunomodulatory effects of Limonium species, it was demonstrated that the polysaccharides of L. sinense improved the phagocyto-sis activity of macrophages in immunosuppressed mice and increased the proliferation and production of IFN-γ and IL-2 by splenocytes in vitro [11]. The extract of L. duriusculum and the isolated flavonoid apigenin inhibited proinflammatory NF-κB-dependent transcriptional responses in HCT116 and THP-1 cells [39]. These observations, as well as the results of the current study, raise the prospect of using the extracts of L. gmelinii and related species for the treatment of diseases associated with immune disorders.…”

Section: Discussionmentioning

confidence: 95%

“…Therefore, UAE could have advantages over CE, as it is significantly faster and simpler and has a lower solvent consumption and "greener" approach [27]. The LC-QToF-MS analysis clearly showed impressive compositions of both extracts, rich in biologically active compounds with remarkable immunomodulatory potential, such as apigenin [39], epigallocatechin-3gallate [20] and quercetin [20]. This work reported, for the first time, that extracts from the above-ground parts of L. gmelinii may modulate the inflammatory mode of murine macrophages and lymphocytes via the stimulation or suppression of TNF-α and IL-6 production and splenocyte proliferation depending on the status of these immune cells.…”

Section: Discussionmentioning

confidence: 99%

“…The identified compounds are summarized in Table 2, including the retention time, molecular formulas, m/z values in both positive and negative modes, and major high-resolution mass fragment ions. As displayed in Table 2, the extracts of the above-ground parts of L. gmelinii were composed of phenolic acid derivatives (sulfated) (1-10); flavonoids ; lignanamides (37)(38)(39)(40)(41)(42)(43)(44)(45)(46); and other compounds, such as amino acids, citric acids and their derivatives, hydroxyquinoline (quinoline derivative), and N-trans feruloyl tyramine (alkaloid) (47-54).…”

Section: Identification and Characterization Of Phenolic Acid Derivat...mentioning

confidence: 99%

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Phytochemical Profiles and In Vitro Immunomodulatory Activities of Extracts Obtained from Limonium gmelinii Using Different Extraction Methods

Kassymova,

Zhusupova,

Ogay

et al. 2023

Plants

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Limonium (L.) gmelinii is a valuable pharmacopoeial Kazakhstani plant. Several studies have reported on the various biological activities of the plant. The purpose of our research was to study and compare the extraction yields, immunomodulatory activities, and chemical compositions of extracts from the above-ground parts of L. gmelinii obtained via conventional extraction (CE; Extract 1) and ultrasound-assisted extraction (UAE; Extract 2). The extracts were characterized by a considerable number of polyphenols and flavonoids: 378.1 ± 4.5 and 382.2 ± 3.3 GAE mg/g, and 90.22 ± 2.8 and 94.61 ± 1.9 QE mg/g in Extract 1 and Extract 2, respectively. Extract 2 had a slightly higher extraction yield (33.5 ± 2.4%) than Extract 1 (30.2 ± 1.6%). Liquid Chromatography–Diode-Array Detection–Electrospray Ionization–Quadrupole Time-of-Flight Mass Spectrometry (LC-QToF-MS) revealed the presence of 54 biologically active compounds in both extracts. It was shown that the studied extracts stimulate the secretion of TNF-α and IL-6 by intact mouse peritoneal macrophages and splenic lymphocytes, whilst they have an inhibitory effect on the secretion of these cytokines by activated immune cells. Both extracts demonstrated similar patterns of stimulation and inhibition in a splenocyte proliferation assay. Altogether, the L. gmelinii extracts obtained via CE and UAE might be suggested as effective immunomodulatory agents. The application of UAE for this purpose seems to be more efficient with a view of obtaining of a highly potent extract in a much shorter time.

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“…Some results found in the literature report characteristics in the leaves of different statice species as high contents of flavonoids, vitamin C, total phenolic compounds and antioxidant activity [6][7] . The plant exhibits antiinflammatory and antitumor action [8][9] , and is employed in the treatment of fever, menstrual disorders and colic 5 .…”

Section: Introductionmentioning

confidence: 99%

Physicochemical attributes and bioactive properties of statice leaves

Souza,

Jung,

Benedicto

et al. 2023

evid.

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Some ornamental plants have been receiving special attention, not only for their use in landscaping, but also because of their bioactive, aromatic and spicy properties. The objective of this study was to characterize physical and chemical attributes and antioxidant activity in leaves of three cultivars of statice (Limonium sinuatum). The cultivars used were QIS White, QIS Blue Dark, and QIS Yellow. The content of total soluble solids (TSS), total titratable acidity (TTA), TSS/TTA ratio, pH, vitamin C, anthocyanin, flavonoids, total phenolic compounds (TPC), total antioxidant activity (TAA) and the relationship between TPC and flavonoids and TAA were analyzed for each cultivar. The results showed that the leaves of the QIS White cultivar have a higher TSS/TTA ratio, indicating better flavor. The QIS White cultivar also showed higher vitamin C and flavonoid content. The cultivars QIS White and QIS Blue Dark showed higher TPC contents, but there were no differences in TTA and anthocyanin content. The average TAA values were 94.3%, indicating that all cultivars have a high capacity to sequester the DPPH radical. There was a positive relationship between TPC with flavonoids and TAA (R2=0.6168 and 0.8826, respectively). The leaves of statice plants, traditionally used in landscaping and as a cut flower, have potential for use in human food.

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Limonium duriusculum (de Girard) Kuntze Exhibits Anti-inflammatory Effect Via NF-κB Pathway Modulation

Abstract: HIGHLIGHTS L. duriusculum n-BuOH extract reduces inflammatory responses both in vitro and in vivo. L. duriusculum n-BuOH extract inhibits NF-κB-dependent transcriptional responses. L. duriusculum n-BuOH extract decreases the expression of TNF-α and IL-6 genes.

Cited by 3 publications

References 51 publications

Synergistic Anti-Inflammatory Effects of Lipophilic Grape Seed Proanthocyanidin and Camellia Oil Combination in LPS-Stimulated RAW264.7 Cells

Synergistic Anti-Inflammatory Effects of Lipophilic Grape Seed Proanthocyanidin and Camellia Oil Combination in LPS-Stimulated RAW264.7 Cells

Phytochemical Profiles and In Vitro Immunomodulatory Activities of Extracts Obtained from Limonium gmelinii Using Different Extraction Methods

Physicochemical attributes and bioactive properties of statice leaves

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