Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

Abstract

Fungi have always attracted a lot of attention as they are able to produce a vast repertoire of enzymes that find a broad spectrum of uses in biotechnological and industrial fields. Undoubtedly, one of the most promising biocatalysts is the lipase, which has been widely used for the biotransformation of a number of commercial products due to its high stability, high catalytic efficiency, versatility and selectivity, making it one of the most attractive and best-studied enzymes. In this study we report the isolation and molecular identification of new lipase-producing fungi from different environmental samples from Morocco. The production and activity of extracellular lipases, at different parameters, was evaluated using the Rhodamine B agar, submerged fermentation and biochemical methods. HIGHLIGHTS  This study focused on new extracellular lipase producing fungi  Identification of the selected lipase-producing fungi was carried out.  Determination of the lipase production in two different media and different pHs.  Study of temperature and pH influence on lipase activity.

Multi-Strain Tropical Bacillus spp. as a Potential Probiotic Biocontrol Agent for Large-Scale Enhancement of Mariculture Water Quality

“…After individual fermentation for 36 h, the fermentation broth of B. flexus QG-3, B. flexus NS-4, and B. licheniformis XCG-6 were separately put into cans for further use ( Supplementary Figure 3 ). The activities of proteinase and lipase in fermentation broth were detected using fat-free milk ( Nygren et al, 2007 ) and rhodamine B ( El Aamri et al, 2020 ), respectively. Bacterial suspension with the appropriate dilution gradient of multi-strain tropical Bacillus spp.…”

“…and rhodamine B(El Aamri et al, 2020), respectively. Bacterial suspension with the appropriate dilution gradient of multi-strain tropical Bacillus spp.…”

Lipases: Sources of Acquisition, Ways of Production, and Recent Applications

“…[80, 81] Rhizopus microsporus [82] Bacillus amyloliquefaciens [83] Pseudomonas cepacia [84] Arthrographis curvata [85] Rhodosporidium babjevae [85] Kluyveromyces marxianus [86]…”

“…The nitrogen sources favor microbial growth, and among the most applied are corn steep liquor, soy flour, yeast extract, peptone, urea, nitrate, and ammonium salts [140]. Many studies used yeast extract as the nitrogen source [55,85].…”

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

“…Lipases also catalyze the reverse reaction by esterification of glycerol and fatty acid. Microbial lipase is produced by many bacteria Bacillus licheniformis [107], Geobacillus thermodenitrificans [108], Pseudomonas aeuriginosa [109], and fungal cells including Aspergillus niger [110,111], Penicillium verrucosum [112], Fusarium solani [113], Arthrographis curvata, and Rhodosporidium babjevae [114]. The remarkable specificity and thermal stability, in addition to the easier recovery procedure of the extracellular fungal lipase over other microbial sources, attracted great attention from the application point of view [115][116][117].…”