Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus

Sanches, Márcio Martinello; Sihler, W.; Silva, Cláudia Efigênia Pereira; Guimarães, Gregório Corrêa; Benito, Norton Pólo; Sosa–Gómez, Daniel Ricardo; Souza, Marlinda Lobo de

doi:10.1590/1678-4324-2019180688

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…In these cases, applications of cooccluded mixtures of HearSNPV and HearMNPV might allow the simultaneous control of both pests, although this required verification in careful field testing. Moreover, other researchers are exploring the application of virus coocclusion technology for the control of complexes of lepidopteran pests in certain crops [58,59]. Commercial producers have also addressed this issue by mixing OBs of different viruses to create products capable of controlling complexes of pests [60].…”

Section: Discussionmentioning

confidence: 99%

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Arrizubieta

Simón

Ricarte-Bermejo

et al. 2022

Viruses

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Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) is a virulent pathogen of lepidopterans in the genera Heliothis and Helicoverpa, whereas Helicoverpa armigera multiple nucleopolyhedrovirus (HearSNPV) is a different virus species with a broader host range. This study aimed to examine the consequences of coocclusion of HearSNPV and HearMNPV on the pathogenicity, stability and host range of mixed-virus occlusion bodies (OBs). HearSNPV OBs were approximately 6-fold more pathogenic than HearMNPV OBs, showed faster killing by approximately 13 h, and were approximately 45% more productive in terms of OB production per larva. For coocclusion, H. armigera larvae were first inoculated with HearMNPV OBs and subsequently inoculated with HearSNPV OBs at intervals of 0–72 h after the initial inoculation. When the interval between inoculations was 12–24 h, OBs collected from virus-killed insects were found to comprise 41–57% of HearSNPV genomes, but the prevalence of HearSNPV genomes was greatly reduced (3–4%) at later time points. Quantitative PCR (qPCR) analysis revealed the presence of HearSNPV genomes in a small fraction of multinucleocapsid ODVs representing 0.47–0.88% of the genomes quantified in ODV samples, indicating that both viruses had replicated in coinfected host cells. End-point dilution assays on ODVs from cooccluded mixed-virus OBs confirmed the presence of both viruses in 41.9–55.6% of wells that were predicted to have been infected by a single ODV. A control experiment indicated that this result was unlikely to be due to the adhesion of HearSNPV ODVs to HearMNPV ODVs or accidental contamination during ODV band extraction. Therefore, the disparity between the qPCR and end-point dilution estimates of the prevalence of mixed-virus ODVs likely reflected virus-specific differences in replication efficiency in cell culture and the higher infectivity of pseudotyped ODVs that were produced in coinfected parental cells. Bioassays on H. armigera, Spodoptera frugiperda and Mamestra brassicae larvae revealed that mixed-virus OBs were capable of infecting heterologous hosts, but relative potency values largely reflected the proportion of HearMNPV present in each mixed-virus preparation. The cooccluded mixtures were unstable in serial passage; HearSNPV rapidly dominated during passage in H. armigera whereas HearMNPV rapidly dominated during passage in the heterologous hosts. We conclude that mixed-virus coocclusion technology may be useful for producing precise mixtures of viruses with host range properties suitable for the control of complexes of lepidopteran pests in particular crops, although this requires validation by field testing.

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Section: Discussionmentioning

confidence: 99%

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Arrizubieta

Simón

Ricarte-Bermejo

et al. 2022

Viruses

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“…O ODV é responsável pela infecção primária no intestino médio do inseto, enquanto os BVs são responsáveis pela infecção secundária ou sistêmica, de célula a célula. Os ODVs ficam encapsulados dentro de uma matriz cristalina protéica (OB), que protege os vírus das adversidades ambientais (SANCHES et al, 2019;SOSA-GÓMEZ et al, 2020).…”

Section: Modo De Açãounclassified

Smart City E Smart Grid No Brasil: O Papel Do Poder Público Na Produção De Cidades Sustentáveis

Brasil,

Silva,

Gouveia

et al. 2023

Meio Ambiente: Agricultura, Desenvolvimento E Sustentabilidade 2

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Editora Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterála de nenhuma forma ou utilizá-la para fins comerciais.Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético. Conselho Editorial Ciências Agrárias e Multidisciplinar

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“…The need for full testing of the efficacy of virus mixtures to control complexes of pests in the field is also a priority for establishing which pests and pest complexes are most amenable to control by mixed-virus technology. This approach has begun to be studied by others, for example for the control of lepidopteran pests in soybean ( Sanches et al, 2019 , 2021 ). Some manufacturers currently provide mixtures of OBs of different variants to overcome resistance in the pest population ( Gueli-Alletti et al, 2017 ).…”

Section: Future Perspectivesmentioning

confidence: 99%

Nucleopolyhedrovirus Coocclusion Technology: A New Concept in the Development of Biological Insecticides

2022

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Nucleopolyhedroviruses (NPV, Baculoviridae) that infect lepidopteran pests have an established record as safe and effective biological insecticides. Here, we describe a new approach for the development of NPV-based insecticides. This technology takes advantage of the unique way in which these viruses are transmitted as collective infectious units, and the genotypic diversity present in natural virus populations. A ten-step procedure is described involving genotypic variant selection, mixing, coinfection and intraspecific coocclusion of variants within viral occlusion bodies. Using two examples, we demonstrate how this approach can be used to produce highly pathogenic virus preparations for pest control. As restricted host range limits the uptake of NPV-based insecticides, this technology has recently been adapted to produce custom-designed interspecific mixtures of viruses that can be applied to control complexes of lepidopteran pests on particular crops, as long as a shared host species is available for virus production. This approach to the development of NPV-based insecticides has the potential to be applied across a broad range of NPV-pest pathosystems.

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Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus

Cited by 6 publications

References 29 publications

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Coocclusion of Helicoverpa armigera Single Nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera Multiple Nucleopolyhedrovirus (HearMNPV): Pathogenicity and Stability in Homologous and Heterologous Hosts

Smart City E Smart Grid No Brasil: O Papel Do Poder Público Na Produção De Cidades Sustentáveis

Nucleopolyhedrovirus Coocclusion Technology: A New Concept in the Development of Biological Insecticides

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