A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis

Carnielli-Queiroz, Lorena; Fernandes, Patrícia Machado Bueno; Fernandes, Antônio Alberto Ribeiro; Ventura, José Aires

doi:10.1590/1678-4324-2019180591

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…Using specific primers, the phytopathogenic fungus Fusarium sp. was identified by a PCR diagnostic protocol in the work of Carnielli-Queiroz, Fernandes, Fernandes, and Ventura (2019). Also according to the authors, the equipment and reagents used for this purpose were able to track samples of pineapple naturally infected with this species.…”

Section: Sensitivity and Specificity Of The Pcr Technique At Differen...mentioning

confidence: 99%

“…For plant phytopathogens, morphological characterization is still the most used method of fungal identification to this day (Carnielli-Queiroz et al, 2019). However, PCR is already being used to identify the species of fungi in food samples, causing a difference in the final quantity of the product (Fungaro & Sartori, 2009).…”

Section: F Verticillioides Detection On Maize Seeds With Different In...mentioning

confidence: 99%

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Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

et al. 2021

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The objective of this study was to evaluate the transmissibility of F. verticillioides, in corn seeds, when inoculated in different phenological stages. The plants were inoculated with the pathogen at the following stages: V7, V9, R1, R2, R4, and R6. The experiments were conducted in pots, in a completely randomized design, with six replications. The fungus infection rate in the seeds was verified through the health test (Blotter Test). The significance of the contrast between the phenological stages and in the two experiments (rainy season and dry season), and productivity showed plant-seed transmission of F. verticillioides in all phenological stages. The PCR technique was able to reveal up to the lowest level, 1% incidence of F. verticillioides in corn seeds. There was a significant difference between the times of plant inoculation and the incidence of the fungus in the seeds. Plants inoculated in the reproductive phase transmitted F. verticillioides for seeds with the highest rate of infection, in the two experiments, in the rainy season (V7 - 48; V9 - 46; R1 - 77.33; R2 - 84; R4 - 96; R6 - 96.67; and Test - 14.67%), and dry season (V7 - 46.67; V9 - 66; R1 - 75.33; R2 - 95.33; R4 - 98; and R6 - 97.33%). In this way, the present work demonstrated that in the reproductive phenological stages R2, R4, and R6, the corn plant was more susceptible to infection with F. verticillioides, and that it also resulted in a decrease in grain productivity.

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Section: Sensitivity and Specificity Of The Pcr Technique At Differen...mentioning

confidence: 99%

Section: F Verticillioides Detection On Maize Seeds With Different In...mentioning

confidence: 99%

Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

et al. 2021

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“…Fusariosis of pineapple is always associated with several species of Fusarium fungi. In Brazil, Fusarium guttiforme has been reported to cause the disease with an estimated loss of about 30-40% (Carnielli-Queiroz et al, 2019). Meanwhile, Jacobs et al (2010) demonstrated F. ananatum as the emerging species involved in pineapple fruit lesions in South Africa.…”

Section: Causal Pathogenmentioning

confidence: 99%

Supplementary 4

2021

Food Res.

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Pineapple is a perennial fruit-bearing tropical plant that belongs to the Bromeliaceae family, which has more than 2500 species. Pineapple is known excellent source of minerals and vitamins. It produces substantial calcium, potassium, glucose, the proteindigesting enzyme bromelain, fibre, vitamin A, B and C. In Malaysia, twelve registered varieties of pineapple have been introduced and commercially planted such as Moris (AC1), Sarawak (AC2), Gandul (AC3), Maspine (AC4), Josapine (AC5) Yankee (AC6) Moris Gajah (AC7), N36 (AC8), MD2 (AC9), View of Sunset (AC10), Madu Kaca (AC11), and Keningau Diamond (AC12). The disease is one of the important factors that can contribute to the huge losses of pineapple yield worldwide. This review paper aimed to discuss the main diseases of pineapple and their control management, particularly in Malaysia. The common diseases of pineapple that cause significant yield losses in the farms such as mealybug wilt-associated virus, bacterial heart rot, fruit collapse, butt rot, fruitlet core rot, black rot, yeasty and fusariosis are highlighted and discussed in detail on the causal pathogens, disease symptoms and signs, disease infection and development. The available control measures for managing pineapple diseases were also included in this paper.

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Current trends, limitations and future research in the fungi?

Hyde,

Baldrian,

Chen

et al. 2024

Fungal Diversity

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The field of mycology has grown from an underappreciated subset of botany, to a valuable, modern scientific discipline. As this field of study has grown, there have been significant contributions to science, technology, and industry, highlighting the value of fungi in the modern era. This paper looks at the current research, along with the existing limitations, and suggests future areas where scientists can focus their efforts, in the field mycology. We show how fungi have become important emerging diseases in medical mycology. We discuss current trends and the potential of fungi in drug and novel compound discovery. We explore the current trends in phylogenomics, its potential, and outcomes and address the question of how phylogenomics can be applied in fungal ecology. In addition, the trends in functional genomics studies of fungi are discussed with their importance in unravelling the intricate mechanisms underlying fungal behaviour, interactions, and adaptations, paving the way for a comprehensive understanding of fungal biology. We look at the current research in building materials, how they can be used as carbon sinks, and how fungi can be used in biocircular economies. The numbers of fungi have always been of great interest and have often been written about and estimates have varied greatly. Thus, we discuss current trends and future research needs in order to obtain more reliable estimates. We address the aspects of machine learning (AI) and how it can be used in mycological research. Plant pathogens are affecting food production systems on a global scale, and as such, we look at the current trends and future research needed in this area, particularly in disease detection. We look at the latest data from High Throughput Sequencing studies and question if we are still gaining new knowledge at the same rate as before. A review of current trends in nanotechnology is provided and its future potential is addressed. The importance of Arbuscular Mycorrhizal Fungi is addressed and future trends are acknowledged. Fungal databases are becoming more and more important, and we therefore provide a review of the current major databases. Edible and medicinal fungi have a huge potential as food and medicines, especially in Asia and their prospects are discussed. Lifestyle changes in fungi (e.g., from endophytes, to pathogens, and/or saprobes) are also extremely important and a current research trend and are therefore addressed in this special issue of Fungal Diversity.

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A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis

Cited by 6 publications

References 34 publications

Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

Supplementary 4

Current trends, limitations and future research in the fungi?

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