Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair

Abstract

Treatment with 2% SDS for 48 h results in 92.54% reduction in DNA contents and complete acellularity of the bubaline diaphragm.• FTIR spectrum of decellularized diaphragm has shown all characteristic transmittance peaks of the collagen.• Bubaline diaphragm matrix shows excellent repair efficiency and biocompatibility for abdominal hernia repair in cattle without complications.• Insert a highlight no longer than 85 characters. HIGHLIGHTS 2 Vora, S.D.; et al.

Preparation, characterization, and xenotransplantation of the caprine acellular dermal matrix

“…Animal tissues are extensively used as scaffolds to repair/regenerate large tissue defects in humans and animals. However, tissue processing is required before its xenogenic use . Animal tissues are typically subjected to de‐cellularization process to remove xenoantigens and preserve extracellular matrix (ECM) bioactivity.…”

Fourier Transform Infrared Spectroscopy of the Animal Tissues

“…The transmittance peaks indicated the presence of organic collagen amide A, amide B, amide I, amide II and amide III chemical functional groups in both native and decellularized aortae. The amide A band (3294 cm −1 ) is associated with H-bonded N-H stretching [11,13,20] and was found at 3282.95 cm −1 for native aorta and 3280 cm −1 for decellularized aorta. The amide B band (2953 and 2928 cm −1 ) is related to CH 2 asymmetric stretching [11,13,21] and was observed at 2958.9 cm −1 for native aorta and 2954.08 cm −1 for decellularized aorta.…”

“…The extracellular matrix (ECM) scaffolds primarily composed of structural collagen protein are widely used in tissue engineering and regenerative medicine [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. These are usually prepared from animal tissues by decellularization process.…”

“…Enzymatic techniques of decellularization include the use of protease (trypsin) [1-5, 8, 10, 12-15], endonucleases and exonucleases. Chemical methods of decellularization include the use of acids and alkalis (acetic acid, peracetic acid, hydrochloric acid, sulfuric acid, ammonium hydroxide), nonionic detergents (Triton X-100), ionic detergents (sodium dodecyl sulfate, sodium deoxycholate, Triton X-200) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], zwitterionic detergents (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, sulfobetaine-10, sulfobetaine-16), organic solvent (Tri(n-butyl)phosphate) [3,10], hypertonic and hypotonic solutions [2,3,8,10,13,15], and chelating agents (EDTA). These reagents at higher concentrations extensively disrupt the structural proteins of ECM scaffolds and make it impossible to analyze by routine techniques [17].…”

“…These reagents at higher concentrations extensively disrupt the structural proteins of ECM scaffolds and make it impossible to analyze by routine techniques [17]. Fourier transform infrared (FTIR) spectroscopy is one of the preferred technique for identification of biomolecules through the study of their characteristic vibrational movements [11,13,14,18]. This technique is simple, reproducible, nondestructive to the tissue, and only small amounts of tissue (micrograms to nanograms) with a minimum preparation are required.…”