Fourier Transform Infrared Spectroscopy of the Animal Tissues

Abstract

Animal tissues are extensively used as scaffolds for tissue engineering and regenerative therapies. They are typically subjected to decellularization process to obtain a cell-free extracellular matrix (ECM) scaffolds. It is important to identify chemical structure of the ECM scaffolds and Fourier transform infrared (FTIR) appears to be a technique of choice. In this chapter, FTIR spectra of native and decellularized buffalo aortae, buffalo diaphragms, goat skin, and native bovine cortical bone are presented. The transmittance peaks are that of organic collagen amide A, amide B, amide I, amide II and amide III chemical functional groups in both native and decellularized aortae, diaphragms and skin. In bone, the transmittance peaks are that of inorganic ν1, ν3 PO43−, OH− in addition to organic collagen amide A, amide B, amide I, amide II and amide III chemical functional groups. These important transmittance peaks of the tissue samples will help researchers in defining the chemical structure of these animal tissues.

The role of decellularized cell derived extracellular matrix in the establishment and culture of in vitro breast cancer tumor model

“…In particular, the peaks centered at 1293 cm −1 and 1077 cm −1 were associated with the asymmetric and symmetric phosphodiester vibrations of nucleic acids while the peak observed at 1151 cm −1 were linked with the hydrogen and non-hydrogen bonds of the C-O stretching vibrations. The chemical structure of CD-dECM was corroborated as the ECM via the existence of the several functional groups in the structure such as organic collagen amide A, amide B, amide I, amide II, and amide III with the help of previous literature findings [21][22][23].…”