Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR

Leão, Evelynne Urzêdo; Spadotti, David Marques de Almeida; Rocha, Kelly Cristina Gonçales; Lima, Élison Fabrício Bezerra; Tavella, Luciana; Turina, Massimo; Krause‐Sakate, Renate

doi:10.1590/1678-4324-2017160425

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…A 433 bp fragment of COI was used as a genetic marker for the identification of the ten thrips species including F. occidentalis , Parthenothrips dracaenae (Heeger), Anaphothrips obscurus (Müller), T. palmi, T. tabaci , T. angusticeps Uzel, Echinothrips americanus Morgan, Hercinothrips femoralis (Reuter), H. haemorrhoidalis (Bouché), and T. picipes (Zetterstedt) [ 32 ]. These markers detected the immature stages as well as adults, even up to 1:120 dilutions without any cross-reactivity to other thrips species [ 55 , 56 ]. In addition to morphology, COI sequences were used to substantiate the incursion of new species, such as H. longisensibilis Xie, Mound, and Zhang in northern Brazil [ 57 ]; and T. parvispinus (Karny) [ 58 ], Podothrips erami Minaei, and F. occidentalis in India [ 59 , 60 ].…”

Section: Pcr-based Identification Of Thrips Using Molecular Markersmentioning

confidence: 99%

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Ghosh

Jangra

Dietzgen

et al. 2021

Insects

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Thrips are insect pests of economically important agricultural, horticultural, and forest crops. They cause damage by sucking plant sap and by transmitting several tospoviruses, ilarviruses, carmoviruses, sobemoviruses, and machlomoviruses. Accurate and timely identification is the key to successful management of thrips species. However, their small size, cryptic nature, presence of color and reproductive morphs, and intraspecies genetic variability make the identification of thrips species challenging. The use of molecular and electronic detection platforms has made thrips identification rapid, precise, sensitive, high throughput, and independent of developmental stages. Multi-locus phylogeny based on mitochondrial, nuclear, and other markers has resolved ambiguities in morphologically indistinguishable thrips species. Microsatellite, RFLP, RAPD, AFLP, and CAPS markers have helped to explain population structure, gene flow, and intraspecies heterogeneity. Recent techniques such as LAMP and RPA have been employed for sensitive and on-site identification of thrips. Artificial neural networks and high throughput diagnostics facilitate automated identification. This review also discusses the potential of pyrosequencing, microarrays, high throughput sequencing, and electronic sensors in delimiting thrips species.

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Section: Pcr-based Identification Of Thrips Using Molecular Markersmentioning

confidence: 99%

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Ghosh

Jangra

Dietzgen

et al. 2021

Insects

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show abstract

Invasive flower thrips, Thrips parvispinus (Karny) occurrence, host expansion and genetic diversification in a tropical poly-crop ecosystem

Palanisamy,

Marimuthu,

Narayanasamy

et al. 2023

Mol Biol Rep

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Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR

Cited by 2 publications

References 19 publications

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

Invasive flower thrips, Thrips parvispinus (Karny) occurrence, host expansion and genetic diversification in a tropical poly-crop ecosystem

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