Bemisia tabaci (Genn.) is one of the most important pests in cultivated areas of vegetables and ornamental crops around the world. Based on the mitochondrial cytochrome oxidize I (mtCOI) sequence, there is evidence that B. tabaci should be considered a cryptic species complex of 11 groups containing 24 species. Two of the groups, Middle East-Asia Minor 1 and Mediterranean include biotypes B and Q, respectively. In this study we evaluated the mtCOI sequence of B. tabaci populations collected in sites of the state of São Paulo, Brazil. Using PCR-RFLP with Taq I, a typical biotype B profile was obtained for all specimens. Based on the comparison with mtCOI reference sequences we found four haplotypes all belonging to the Middle EastAsia Minor 1. They occurred in the hosts pepper (Capsicum annuum L.), tomato (Solanum lycopersicum L.), eggplant (Solanum melongena L.) and cucurbitaceae plants.
From Janu a ry/2 00 7 to July/20 0 8 a survey wa s ca rried ou t to evaluate the occurrence of begomoviruses in pepper and tomato crops from São Paulo State. Total DNA was extracted from 710 pepper and 103 toma to samples, and the presence of begomoviru ses wa s tested by Polymerase chain reaction (PCR). The same samples were tested by Rolling Circle Amplification (RCA) followed by PCR, and some positive sa mples a na lyzed by RCA-RFLP a nd clea ved by the restriction enzyme HpaII to evaluate the genetic variability of these isolates. By PCR, 99 (13.94%) samples collected from pepper and 39 (3 7 .8 6 %) from toma to were positives for the presence of begomovirus, while by RCA-PCR 3 33 (46 .9 0%) and 82 (79 .6 1%) RESUMOUm levantamento pa ra avaliar a ocorrência de begomovírus nas culturas de pimentão e tomateiro no estado de São Paulo foi realizado entre janeiro/2007 e julho/2008. O DNA total de amostras de pimentão (710) e de tomateiro (103) foi extraído e a presença de begomovírus foi testa da por PCR. Pa ra lela mente, a s mesma s a mostra s fora m a va liadas por a mplifica çã o por círculo rola nte (RCA) segu idas de PCR, e algumas amostras positivas analisadas por RCA-RFLP com a enzima de restriçã o Hp a II, a fim de se conhecer a va ria bilida de genética dos isolados. Os resultados demonstraram que, para a técnica de PCR, 9 9 a mostra s de pimentã o (1 3 ,9 4 %) e 3 9 de toma teiro (37,86%) foram positivas para a presença de begomovírus, enquanto Rocha, K.C.G.; Marubayashi, J.M.; Navas-Castillo, J.; Pavan, M.A.; Krause-Sakate, R. Ocorrência e variabilidade genética do Tomato severe rugose virus em tomateiro e pimentão no Estado de São Paulo. Summa Phytopathologica, v.36, n.3, p.222-227, 2010. from pepper and tomato, respectively, indicating higher sensitivity of this technique. The 5' region of the coat protein (CP) gene and a segment of the intergenic region was analyzed indicating the presence of Tomato severe rugose virus (ToSRV) in pepper and tomato plants. However, the partial sequencing of clones from RCA products from a toma to sa mple indica ted mixed infection of ToSRV with To ma to yellow vein streak virus (ToYVSV). By RCA-RFLP four restriction profiles were observed for ToSRV in pepper, while 18 profiles for begomovirus from tomato plants, indicating higher degree of genetic variability for begomovirus found in tomato plants compared to that in pepper pla nts.O tomateiro (Solanum lycopersicum L.) e pimentão (Capsicum annuum L.) são as hortaliças mais cultivadas no Brasil. No entanto, as doenças causadas por vírus têm preocupado produtores devido às perdas ocasionadas. Dentre estes destacam-se os begomovírus pertencentes à Família Geminiviridae (10).A família Geminiviridae é constituída por quatro gêneros: Mastrevirus, Curtovirus, Topocuvirus e Begomovirus (26). Os vírus pertencentes ao gênero Begomovirus são considerados os mais importantes pelo número de espécies que infectam plantas cultivadas e invasoras (7, 10), constituindo-se atualmente num dos principais problemas fitossanit...
RESUMO O presente trabalho teve por objetivo estudar os aspectos biológicos, morfológicos e comportamentais de uma linhagem unipariental de Aspidiotus nerii
Powdery mildew is one of the most commom foliar diseases of cultivated and wild cucurbits worldwide. Six different fungal species have been reported to be associated with powdery mildew of cucurbits, such as Podosphaera xanthii and Golovinomyces cichoracearum. The aim of the study was to identify the causal agent of the disease in watermelon plants (Citrullus lanatus) cultivated in greenhouses of the State of São Paulo. DNA total was extracted using Chelex method and the ITS region was amplified via standard PCR reaction using the specific primers PMITS1 and PMITS2. A fragment of 700pb was amplified and the sequence showed 99% of identity with P. xanthii (accession number JQ728480). Morphological observation showed that the conidia were ellipsoidal-ovoid, had fibrosin bodies and a bifurcated germinative tube in the lateral position. These characteristics and the molecular analyses confirmed P. xanthi as the etiological agent of powdery mildew in watermelon plants in São Paulo State. Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state
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