Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity

Warda, E. Ashour; Aty, Abeer A. Abd El; Hamed, Eman R.; Mahmoud, Ahmed S.; Ahmed, Ishtiaq

doi:10.1590/1678-4324-2016160245

Cited by 13 publications

(6 citation statements)

References 27 publications

(18 reference statements)

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“…Table 2 shows a wide variation on the enzymatic activity between the different combinations of factors. This agreed with various studies that reported wide ranges in the response at this stage of the analysis, supporting the need for further optimization [31][32]. The Plackett-Burman design is useful as a first screening since it only considers main effects.…”

Section: Results and Discussion 1) Screening Of Key Factors For Lacca...supporting

confidence: 88%

An Optimal Culture Medium for Laccase Production and Sugar Cane Vinasse Biotreatment with Trametes villosa Using Plackett-Burman and Central Composite Designs

Elena Caballero,

Jimenez,

Miranda

et al. 2024

App. Envi. Res.

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The optimal conditions for laccase production and vinasse biotreatment with a native strain of Trametes villosa were determined by a screening-optimization approach. Eleven factors including nutrient concentration, vinasse dilution (%v/v), inoculum volume, carbon to nitrogen ratio and initial pH, were investigated for their effects on laccase activity applying the Plackett-Burman screening design. The selected factors were optimized using a central composite design, and then evaluated on a vinasse biotreatment experiment. The factors that contributed the most to the enzymatic activity were the concentrations of MgSO4∙7H2O (A), FeSO4∙7H2O (B) and CuSO4∙5H2O (C), alongside initial pH. After 10 days, laccase activity was 544.038 U L-1 for the following concentrations of A, B and C: 0.250 g L-1, 0.020 mg L-1, and 0.100 g L-1, respectively. Vinasse biotreatment under optimized conditions resulted in 82.74%, 78% and 75.97% of phenol, color, and COD removal respectively, while final pH value was 6.90. These results showed that the native strain of T. villosa has a good potential for further research on laccase production and vinasse sustainable management.

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Section: Results and Discussion 1) Screening Of Key Factors For Lacca...supporting

confidence: 88%

An Optimal Culture Medium for Laccase Production and Sugar Cane Vinasse Biotreatment with Trametes villosa Using Plackett-Burman and Central Composite Designs

Elena Caballero,

Jimenez,

Miranda

et al. 2024

App. Envi. Res.

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“…P. putida DSMZ 548 has a high capability to degrade rather recalcitrant and inhibiting xenobiotics 27 , and related strains are successfully used for the production of bio-based polymers and a broad range of chemicals 28 . B. diminuta ATCC 11568 is a relevant coenzyme Q10 producer 29 , a valuable molecule for pharmaceutical and cosmetic applications 30 , and related strains are used as well in the production of other fine chemicals, enzymes and in bioremediation [31][32][33] . S. fuliginis ATCC 27551, previously known as Flavobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

“…B. diminuta ATCC 11568 is a relevant coenzyme Q10 producer, a valuable molecule for pharmaceutical and cosmetic applications, and related strains are used in the production of other fine chemicals, enzymes, and in bioremediation. − S. fuliginis ATCC 27551, previously known as Flavobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

Tamayo-Ramos

Rumbo

Caso³

et al. 2018

ACS Appl. Mater. Interfaces

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Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA.

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“…Thus, the composition of the culture medium can affect chitinase production. 16 The nitrogen source and fermentation conditions impact chitinase synthesis in marine Bacillus sp. R2.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Kurniawan¹,

Leelakriangsak²,

Panphon³

2022

J. Pure Appl. Microbiol.

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This study aimed to optimize the medium compositions and cultural conditions for improved chitinase production by a potential strain of Bacillus isolated from the marine environment and determine the antifungal activity of its chitinase against plant pathogenic fungi. Five potential isolates were cultured for chitinase production by submerged fermentation using colloidal chitin in a liquid medium. In this study, chitinase activity was determined by measuring reducing sugars, which were determined by the 3,5-dinitrosalicylic acid (DNS) assay. The most potential isolate, B26, showed similarity to Bacillus paramycoides based on the 16S rRNA gene sequence. The maximum chitinase production was achieved at 6.52±0.02 U/mL after 72 h of incubation in a medium containing 2% squid pen powder, supplemented with 0.5% sodium nitrate and 2% NaCl, with an initial pH of 7. It was observed that the optimization of cultural conditions resulted in 2.83 times higher chitinase production than an unoptimized medium. The antifungal activity of crude chitinase against phytopathogenic fungi was evaluated by a well-diffusion method. The chitinase of B. paramycoides B26 effectively inhibited the growth of Fusarium solani TISTR 3436 (83.4%) and Penicillium chrysogenum TISTR 3554 (80.12%).

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Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity

Abstract: ABSTRACT

Cited by 13 publications

References 27 publications

An Optimal Culture Medium for Laccase Production and Sugar Cane Vinasse Biotreatment with Trametes villosa Using Plackett-Burman and Central Composite Designs

An Optimal Culture Medium for Laccase Production and Sugar Cane Vinasse Biotreatment with Trametes villosa Using Plackett-Burman and Central Composite Designs

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

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