Genetic Variability of Brycon hilarii in a Repopulation Program

Bignardi, Annaíza Braga; Povh, Jayme Aparecido; Alves, Mariana Srucki; Goes, Elenice Souza dos Reis; Filho, Ruy Alberto Caetano Corrêa; Castro, Reinaldo José de; Barrero, Nelson Mauricio Lopera

doi:10.1590/1678-4324-2016160102

Cited by 4 publications

(5 citation statements)

References 20 publications

(19 reference statements)

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“…The size of alleles produced by primers derived from the genus Brycon was similar to that in previous research , as B. hilarii (SANCHES & GALETTI, 2006;BIGNARDI et al, 2016) results are close to those observed by these authors and showed moderate genetic variability. The mean value of He was higher than the value of Ho, which likely inferred the significant deviation in the Hw, indicating a heterozygous deficit through the Fis coefficient in most of loci.…”

Section: Discussionsupporting

confidence: 89%

Cross-amplification of heterologous microsatellite markers in Piracanjuba

et al. 2017

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Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

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Section: Discussionsupporting

confidence: 89%

Cross-amplification of heterologous microsatellite markers in Piracanjuba

et al. 2017

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“…In a study with natural populations of Prochilodus lineatus, Lopera- Barrero et al (2016b) found a variation of three to six alleles produced by the Par12 locus and three to five alleles produced by the Par80 locus. The number of alleles produced in our study was lower than that reported by Bignardi et al (2016) for Brycon hilarii using the loci Bh8, Bh13, and Bh16. The same applies to the primers Par80 and Cm1A8, which showed a lower ACTA AMAZONICA number of alleles than that found in the literature (Barbosa et al 2008;Lopera-Barrero et al 2016b;Santos et al 2009).…”

Section: Discussioncontrasting

confidence: 87%

“…Genetic analyzes of populations on a wider geographic scale will be necessary to estimate variability and the level of genetic structure in this species, and to evaluate the possibility of inbreeding in shoals, which would raise F IS values. The size of the alleles produced are close to the values reported by Bignardi et al (2016) for B. hilarii. The primer Bag22 also produced a similar size to that found by Araújo (2012) for B. amazonicus.…”

Section: Discussionsupporting

confidence: 85%

“…In addition, they can be used for restocking programs to control breeder mating and produce fingerlings with high VOL. 50(3) 2020: 232 -238 ACTA AMAZONICA genetic diversity (Bignardi et al 2016). The breeding and genetic control of this species in captivity could reduce fishing pressure and the demand for wild individuals (Oliveira et al 2018), contributing to the conservation of the species.…”

Section: Discussionmentioning

confidence: 99%

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Transferability of heterologous primers in Brycon falcatus

et al. 2020

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The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species.

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“…number of alleles per locus was also similar to that reported in the cross-amplification in B. orbignyanus at some loci such as bom13 (cArmo et al, 2015;cAsTro et al 2017), bh13, bom5, andPar80 (cAsTro et al, 2017). contrarily, the number of alleles was lower relative to those reported from studies that used species-specific primers of B. opalinus and B. hilarii (bArroso et al, 2003;biGnArDi et al, 2016). other studies have demonstrated that the genus Prochilodus also has a high plasticity of microsatellite loci transfer (bArbosA et al, 2008;LoPerA-bArrero et al, 2016b), even among different genera, as shown by cAsTro et al 2017, who observed that Par80, the same loci amplified for B. gouldingi, showed crossamplification in B. orbignyanus.…”

Section: Discussionmentioning

confidence: 69%

Transferability of heterologous microsatellite primers in Brycon gouldingi

et al. 2018

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Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were assessed. Among the 30 primers, seven showed satisfactory transferability, six of which belonged to the genus Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13, and Borg59 (B. orbignyanus), and one belonged to P. argenteus (Par80). The primers for the other species tested showed non-specificity or monomorphism; and were therefore, excluded from the analyses. The number of alleles ranged between two (Borg13 and Borg59) and three (Bh13, BoM5, BoM13, Borg9 and Par80), with sizes varying between 103 bp (BoM5) and 430 bp (Borg9). Four primers showed evidence of null alleles (BoM13, Borg9, Borg13, and Par80), which could probably be attributed to the respective Hardy-Weinberg deviation. Thus, seven primers were validated for cross-amplification in B. gouldingi, which may be used in future studies involving this species.

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Genetic Variability of Brycon hilarii in a Repopulation Program

Cited by 4 publications

References 20 publications

Cross-amplification of heterologous microsatellite markers in Piracanjuba

Cross-amplification of heterologous microsatellite markers in Piracanjuba

Transferability of heterologous primers in Brycon falcatus

Transferability of heterologous microsatellite primers in Brycon gouldingi

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