Variation of Hydroxyapatite Content in Soft Gelatin Affects Mesenchymal Stem Cell Differentiation

Kantawong, Fahsai; Tanum, Junjira; Wattanutchariya, Wassanai; Sooksaen, Pat

doi:10.1590/1678-4324-2016150650

Cited by 5 publications

(4 citation statements)

References 36 publications

(39 reference statements)

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“…A study by Kantawong et al showed that addition of nano-hydroxyapatite (HA) could enhance cell adhesion on a soft substrate and, maybe together with the releasing of Ca 2+ , induced a cell response to change gene expression and differentiation. Gelatin with 1.0 mg/mL supported neuronal gene expression in HMSCs, compared with pure gelatin and gelatin with 0.5 mg/mL of HA [53]. These studies supported that the influence of modulus on NSC differentiation that control stem cell fate would be possible for applications in reprogramming and cellular transdifferentiation.…”

Section: Biomimetic Biomaterials For Fibroblast Reprogrammingmentioning

confidence: 56%

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The Potential of Fibroblast Transdifferentiation to Neuron Using Hydrogels

Kantawong

2021

Processes

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Currently there is a big drive to generate neurons from differentiated cells which would be of great benefit for regenerative medicine, tissue engineering and drug screening. Most studies used transcription factors, epigenetic reprogramming and/or chromatin remodeling drugs which might reflect incomplete reprogramming or progressive deregulation of the new program. In this review, we present a potential different method for cellular reprogramming/transdifferentiation to potentially enhance regeneration of neurons. We focus on the use of biomaterials, specifically hydrogels, to act as non-invasive tools to direct transdifferentiation, and we draw parallel with existing transcriptional and epigenetic methods. Hydrogels are attractive materials because the properties of hydrogels can be modified, and various natural and synthetic substances can be employed. Incorporation of extracellular matrix (ECM) substances and composite materials allows mechanical properties and degradation rate to be controlled. Moreover, hydrogels in combinations with other physical and mechanical stimuli such as electric current, shear stress and tensile force will be mentioned in this review.

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Section: Biomimetic Biomaterials For Fibroblast Reprogrammingmentioning

confidence: 56%

“…Moreover, GA residuals can be harmful to the cultured cells. This problem was solved by soaking hydrogels in water before lyophilization to remove all GA residuals [52,53]. Various methods and principles about hydrogel crosslinking were provided by Hu et al [54].…”

Section: Biomimetic Biomaterials For Fibroblast Reprogrammingmentioning

confidence: 99%

The Potential of Fibroblast Transdifferentiation to Neuron Using Hydrogels

Kantawong

2021

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“… 19 Furthermore, it was shown that the biomaterials from the study of Tanum et al 19 could induce a neural differentiation in the HMSCs. 20 The present study aimed to demonstrate that the gelatin-hydroxyapatite-pig brain biomaterials could induce the expression of the reprogramming factor Klf4 and nerve transcription factors, such as NFIa, NFIb, and SOX9 in fibroblasts. Thus, we hypothesized that biomaterials could be used effectively and economically as a tool to generate iNSCs from fibroblasts.…”

Section: Introductionmentioning

confidence: 96%

Reprogramming of mouse fibroblasts into neural lineage cells using biomaterials

Kantawong¹,

Saksiriwisitkul²,

Riyapa³

et al. 2018

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Introduction: Induced neural stem cells (iNSCs) have the ability of differentiation into neurons, astrocytes and oligodendrocytes. iNSCs are very useful in terms of research and treatment. The present study offers an idea that biomaterials could be one of the tools that could modulate reprogramming process in the fibroblasts. Methods: Gelatin biomaterials were fabricated into 3 types, including (i) gelatin, (ii) gelatin with 1 mg/mL hydroxyapatite, and (iii) gelatin with hydroxyapatite and pig brain. NIH/3T3 fibroblasts were cultured on each type of biomaterial for 7, 9 and 14 days. RT-PCR was performed to investigate the gene expression of the fibroblasts on biomaterials compared to the fibroblasts on tissue culture plates. PI3K/Akt signaling was performed by flow cytometry after 24 hours seeding on the biomaterials. The biomaterials were also tested with the human APCs and PDL cells. Results: The fibroblasts exhibited changes in the expression of the reprogramming factor; Klf‫4 and the neural transcription factors; NFIa, NFIb and Ptbp1 after 9 days culture. The cultivation of fibroblasts on the biomaterials for 7 days showed a higher expression of the transcription factor SOX9. The expression of epigenetic genes; Kat2a and HDAC3 were changed upon the cultivation on the biomaterials for 9 days. The fibroblasts cultured on the biomaterials showed an activation of PI3K/Akt signaling. The human APCs and human PDL cells developed mineralization process on biomaterials Conclusion: Changes in the expression of Klf4, NFIa, NFIb, Ptbp1 and SOX9 indicated that fibroblasts were differentiated into an astrocytic lineage. It is possible that the well-designed biomaterials could work as powerful tools in the reprogramming process of fibroblasts into iNSCs.

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“…A previous study showed that photocurable hydrogel made from pure gelatin possessed ultrasoft property which induced neural differentiation in adipose derived stem cells (Kantawong et al, 2015). When hydroxyapatite was added to pure gelatin, it was found that the material could induce neural differentiation in hMSCs (Kantawong et al, 2016). If gelatin was applied on its own as the material of the scaffold, it would not be possible to obtain the kind of scaffold that could provide enough mechanical and physical properties for wound regeneration.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Blended Gelatin–Polyvinyl Alcohol–Chitosan Scaffold for Wound Regeneration

Yin¹,

Udomsom²,

Kantawong³

2020

CMUJNS

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A single type polymer had very limited function and property which was not enough to be applied in the complex real situation. This study aimed to prepare the composited scaffolds by blending the gelatin, polyvinyl alcohol and chitosan together. The blended scaffolds were fabricated by making the final concentration of 7% gelatin, 0.5 % PVA and 0.1% chitosan and crosslinking by glutaraldehyde. The Young's modulus was investigated by using atomic force microscopy (AFM). The pore size was investigated using the scanning electron microscope (SEM). The swelling rate of scaffolds were tested by water displacement method. The degradation rate of the scaffolds was studied using lysozyme digestion. The MTT assay was applied within this study in order to find out the relative cell viability upon culturing with the gelatin and the blended scaffolds compared to tissue culture plates. Collagen type IV expression was investigated in mouse fibroblasts cultured on both scaffolds for 10 days using real time PCR. The results showed that Young's moduli of gelatin and blended scaffold were 53.30±26.80 kPa and 98.01±17.50 kPa, respectively. The average pore size of gelatin and blended scaffolds were 336.33±52.25 μm and 68.17±8.91 μm, respectively. The sample's porosity of gelatin and blended scaffolds were 85. 41±2. 11% and 21. 48±1. 01% , respectively. The swelling rate and the degradation rate of gelatin scaffold were higher than blended scaffold. The MTT

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Variation of Hydroxyapatite Content in Soft Gelatin Affects Mesenchymal Stem Cell Differentiation

Abstract: Gelatin is a common material used in tissue engineering

Cited by 5 publications

References 36 publications

The Potential of Fibroblast Transdifferentiation to Neuron Using Hydrogels

The Potential of Fibroblast Transdifferentiation to Neuron Using Hydrogels

Reprogramming of mouse fibroblasts into neural lineage cells using biomaterials

Fabrication of Blended Gelatin–Polyvinyl Alcohol–Chitosan Scaffold for Wound Regeneration

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