Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants

Al-Rasheed, Nouf M.; Fadda, Laila; Ali, Hanaa M.; Yacoub, Hazar

doi:10.1590/1678-4324-2016150553

Cited by 10 publications

(8 citation statements)

References 52 publications

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“…Chlorogenic acid an ester of caffeic acid and quinic acid (3-O-caffeoylquinic acid (CGA)) possess significant antioxidant activity as well as antibacterial, anticarcinogenic, antiinflammatory, antihypertension activities [22,23] . Chlorogenic acid is one of the most abundant hydroxycinnamic acid derivative in human diet and is widely distributed in medicinal plants, a number of fruits and vegetables as well as daily beverages like coffee, tea, wine, and tobacco [22,24] .…”

Section: Resultsmentioning

confidence: 99%

“…Chlorogenic acid is one of the most abundant hydroxycinnamic acid derivative in human diet and is widely distributed in medicinal plants, a number of fruits and vegetables as well as daily beverages like coffee, tea, wine, and tobacco [22,24] . Related to its hepatoprotective activity many studies have been reported besides its other biological activities.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore pretreatment of chlorogenic acid protected the PC12 cells from β-amyloid (Aβ) which are reported to reduced viability of PC12 cells by reducing the level of intracellular Ca +2 and apoptosis related of proteins. Chlorogenic acid was reported to potentiate the antiapoptotic and antifibrogenic effects of silymarin and this combination increased the expression of Bcl-2 down regulated by CCl 4 [22] . In current study chlorogenic acid was also investigated for its hepatoprotective activity in acute hepatotoxicity test model induced by CCl 4 together with hydrangenol-8-O-β-glucoside and scorzotomentosin-4'-O-β-glucoside.…”

Section: Resultsmentioning

confidence: 99%

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Preclinical Evaluation of Scorzonera sp. Root Extracts and Major Compounds Against Acute Hepatotoxicity Induced by Carbon Tetrachloride

Acikara¹,

Yigitbasi²,

Sakul³

et al. 2017

pharmaceutical-sciences

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Evaluation of hepatoprotective activities of Scorzonera roots and their major compounds, was aimed in current study. Scorzonera latifolia, S. tomentosa, S. mollis ssp. szowitsii, S. parviflora and S. cana var. jacquiniana roots, methanol-water (80:20) extracts together with chlorogenic acid, scorzotomentosin-4'-O-β-glucoside, hydrangenol-8-O-β-glucoside as major compounds isolated from S. latifolia roots were tested for their hepatoprotective activities. Sprague Dawley rats were used for experiment and hepatotoxicity was induced by carbon tetrachloride. Aspartate aminotransferase and alanine aminotransferase levels were measured and all results were confirmed by histopathological examination. Plasma aspartate aminotransferase and alanine aminotransferase levels of examined groups were not significant when compared to carbon tetrachloride-treated groups. However histopathological results have revealed that all tested groups have less damage when compared to carbon tetrachloride group except scorzotomentosin-4'-O-β-glucoside and hydrangenol-8-O-β-glucoside groups. Scorzonera species displayed moderate hepatoprotective activities against carbon tetrachloride induced acute toxicity. Chlorogenic acid, among tested compounds exhibited higher activity than all tested Scorzonera species as well as other isolated compounds. Therefore chlorogenic acid could be suggested as responsible compound.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Preclinical Evaluation of Scorzonera sp. Root Extracts and Major Compounds Against Acute Hepatotoxicity Induced by Carbon Tetrachloride

Acikara¹,

Yigitbasi²,

Sakul³

et al. 2017

pharmaceutical-sciences

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“…Such dramatic muscle damage, necrosis, was not observed in animals treated with MLT, which suggests that MLT was able to increase all skeletal muscle cells ability to cope with an increase in ROS production. Besides necrosis a significant increase in ROS, seen after CCl 4 application, can induce cell apoptosis (Yu et al, 2005), which is characterized by an increase in pro-apoptotic protein (Bax), TGF-β and Smad-2 expression (Al-Rasheed et al, 2016). Previous studies evaluated the potential of MLT in preventing anti-Fas antibodies induced cell damage, where the MLT almost fully prevented an increase in Bax expression (Kocic et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effects of melatonin on oxidative stress parameters and pathohistological changes in rat skeletal muscle tissue following carbon tetrachloride application

Sokolović

Lilić²,

Milenković³

et al. 2018

Saudi Pharmaceutical Journal

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Animal models demonstrating skeletal muscle (SM) disorders are rarely investigated, although these disorders accompany liver disorders and can occur during prolonged exercise/training. In cases of SM disorders exogenous antioxidants, such as melatonin, could help by generally improving tissues antioxidant capacities. We aimed to analyze the potential of melatonin in preventing biochemical and structural changes in rat biceps muscle (BM) occurring after an acute exposure to carbon tetrachloride (CCl4). Biceps muscles obtained from male Wistar rats belonging to different experimental groups were biochemically (determination of tissue MDA, total antioxidant capacity, GSH, CAT, SOD and GPx activities) and pathologically analyzed. Also, serum levels of potassium, LHD and CK were analyzed in all experimental animals. The obtained results were statically compared with those from vehicle-treated control group. The applied melatonin prevented potassium and intracellular enzyme leakage (CK and LDH) that was induced by CCl4, as well as an increase in tissue MDA. From a panel of determined oxidative stress parameters melatonin was able to statistically significantly prevent changes in total antioxidative capacity and in CAT, SOD and GPx activities induced by CCl4. Microscopic analysis of BM from the animals exposed to CCl4 revealed significant muscle fiber disorganization and massive inflammatory cell infiltration. All these changes were significantly ameliorated in the group that received melatonin prior to CCl4. Changes in serum and tissue biochemical parameters accompanied the observed pathological changes, which demonstrated a significant influence of melatonin in preventing skeletal muscle damage induced by CCl4.

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“…63 The ROS and free radicals enhance both apoptosis and necrosis in liver tissue and cause increase in TGF-β mRNA expression and decrease in Bcl-2 mRNA expression. 50 The signals include oxidative stress cause changes in organelle architecture and membrane permeability, as well as mitochondrial proteins (including cytochrome c) which are discharged into the cytosol and considered to be the most critical controllers of the intrinsic pathway. The CCl 4 induce an oxidative stress on mitochondria took place.…”

Section: Detection Of Caspase-9 and Bcl-2 In Liver And Kidney Tissuesmentioning

confidence: 99%

Propolis nanoparticles modulate the inflammatory and apoptotic pathways in carbon tetrachloride‐induced liver fibrosis and nephropathy in rats

Izzularab

Megeed

Yehia

2020

Environmental Toxicology

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This study assessed the use of emulsion-produced propolis nanoparticles for treating carbon tetrachloride (CCl 4)-induced liver fibrosis and nephropathy on albino rat model. The evaluation of hepatotoxicity, nephrotoxicity, and the treatment outcomes involved biochemical investigations of blood samples as well as molecular analysis, and histopathological assessment for liver and kidney tissue samples. CCl 4 treatment caused elevated biochemical indicators of hepatotoxic and nephrotoxic effects as detected by liver and kidney functions tests, which improved gradually with propolis nanoparticles treatment. The molecular studies showed an increase in transforming growth factor β (TGF-β), Nephrin, and Caspase-9, while Bcl-2 levels dropped in both liver and kidney tissue samples; such changes were normalized after treatment. The histological findings confirm both biochemical and molecular studies. Our results indicated that propolis nanoparticles had an anti-inflammatory effect as proved by decreased expression of TGF-β in liver tissue and Nephrin in kidney tissue. The propolis nanoparticles showed an anti-apoptotic effect on liver and kidney tissue increasing the expression of Bcl-2 and decreasing the expression of Caspase-9.

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Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants

Abstract: ABSTRACT

Cited by 10 publications

References 52 publications

Preclinical Evaluation of Scorzonera sp. Root Extracts and Major Compounds Against Acute Hepatotoxicity Induced by Carbon Tetrachloride

Preclinical Evaluation of Scorzonera sp. Root Extracts and Major Compounds Against Acute Hepatotoxicity Induced by Carbon Tetrachloride

Effects of melatonin on oxidative stress parameters and pathohistological changes in rat skeletal muscle tissue following carbon tetrachloride application

Propolis nanoparticles modulate the inflammatory and apoptotic pathways in carbon tetrachloride‐induced liver fibrosis and nephropathy in rats

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