Viable offspring after successful non-surgical embryo transfer in goats

Fonseca, J. F. da; Esteves, L. V.; Zambrini, F. N.; Brandão, Felipe Zandonadi; Peixoto, Maria Gabriela Campolina Diniz; Verneque, R.S.; Siqueira, L. G. B.; Viana, J. H. M.

doi:10.1590/1678-41626783

Cited by 15 publications

(15 citation statements)

References 7 publications

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“…It is unlikely that a combination of hormonal drugs used in this study exerted negative effects on embryo viability although their direct influence on embryo health remains to be tested. Earlier studies have shown that treatment of goats with PGF 2 α (Fonseca et al., ), PGF 2 α+OT (Pereira, Sohnrey, & Holtz, ) or ewes with EB+OT (Wulster‐Radcliffe, Costine, & Lewis, ) prior to embryo recovery did not adversely affect embryo quality. Transfer of embryos recovered from donor goats pretreated with cloprostenol for 16 hr prior to embryo recovery resulted in nearly 60% pregnancy rate when both the fresh or frozen‐thawed, good and excellent quality embryos were used (Fonseca et al., ).…”

Section: Discussionmentioning

confidence: 95%

“…It is unlikely that a combination of hormonal drugs used in this study exerted negative effects on embryo viability although their direct influence on embryo health remains to be tested. Earlier studies have shown that treatment of goats with PGF 2 α (Fonseca et al, 2014), PGF 2 α+OT (Pereira, Sohnrey, & Holtz, 1998) TA B L E 3 Number of Santa Inês ewes' responses (Grades 1-5) for degree of difficulty to transverse the uterine cervix at oestrus and prior to embryo flushing (6-7 days after the onset of oestrus). Embryo recovery was attempted after an application of the cervical dilation protocols * using oestradiol benzoate (EB) administered by intramuscular (EBim) or intravaginal (EBivg) route cervix, Grade 3 to the intermediated cervix and Grades 4 and 5 to the highly asymmetrical cervix.…”

Section: End Points Ebim Ebivgmentioning

confidence: 99%

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Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Fonseca

Zambrini

Guimarães

et al. 2018

Reprod Domestic Animals

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This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7-day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d-cloprostenol by latero-vulvar route 7 days apart. After the second injection of d-cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d-cloprostenol (latero-vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d-cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous-synchronized Santa Inês ewes pretreated with d-cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.

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Section: Discussionmentioning

confidence: 95%

“…It is unlikely that a combination of hormonal drugs used in this study exerted negative effects on embryo viability although their direct influence on embryo health remains to be tested. Earlier studies have shown that treatment of goats with PGF 2 α (Fonseca et al, 2014), PGF 2 α+OT (Pereira, Sohnrey, & Holtz, 1998) TA B L E 3 Number of Santa Inês ewes' responses (Grades 1-5) for degree of difficulty to transverse the uterine cervix at oestrus and prior to embryo flushing (6-7 days after the onset of oestrus). Embryo recovery was attempted after an application of the cervical dilation protocols * using oestradiol benzoate (EB) administered by intramuscular (EBim) or intravaginal (EBivg) route cervix, Grade 3 to the intermediated cervix and Grades 4 and 5 to the highly asymmetrical cervix.…”

Section: End Points Ebim Ebivgmentioning

confidence: 99%

Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes

Fonseca

Zambrini

Guimarães

et al. 2018

Reprod Domestic Animals

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“…It is important to highlight that we believe there are important advantages in the use of goat as a model, such as the low cost of maintaining a goat and its easy of handling compared to other productive species (e.g., cow). Moreover, the success of oocyte/ embryo transfer and recovery in the goat species is well recognized (FONSECA et al, 2013(FONSECA et al, , 2014(FONSECA et al, , 2016.…”

Section: Resultsmentioning

confidence: 99%

“…The female goat was prepared to receive COCs, as described earlier. COCs were transferred (FONSECA et al, 2014) and recovered (FONSECA et al, 2013) through a transcervical route. Briefly, under sedation and anesthesia, the goat was kept in a standing position and contained within an appropriate box.…”

Section: Cocs Transfer and Recoverymentioning

confidence: 99%

“…In the same way, the intra-follicular transfer of oocytes showed interesting results, but requires specialist skill to transfer oocytes into the follicular antrum (SPRÍCIGO et al, 2016). Thus, our group has proven the feasibility of nonsurgical embryo transfer techniques in small ruminants (FONSECA et al, 2014) and, in this study, we used this new technique to perform the maturation of bovine oocytes in a goat's uterus, whilst aiming to establish an efficient and practical method of ex situ maturation for bovine oocytes. This study is the first report to use a goat as a live incubator for the maturation of bovine oocytes.…”

Section: Introductionmentioning

confidence: 97%

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Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?

Fonseca

Batista

Trevizan

et al. 2019

SCA

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We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO 2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat's uterus could present an alternative to nuclear maturation.

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