Serum concentrations of acute phase proteins in goats and ewes with naturally acquired Staphylococcus aureus mastitis

Abstract

Serum protein concentrations, including acute phase proteins (APPs), of goats and ewes with naturally acquired Sthaphylococcus aureus mastitis were determined by means of SDS-PAGE electrophoresis to evaluate the relevance of these APPs as biomarkers of the disease in these species. Fifteen healthy goats and 5 goats with naturally acquired staphylococci mastitis, as well as fifteen healthy ewes and 5 ewes with staphylococci mastitis were submitted to daily blood sampling during 7 days. In goats, an increase of 570%, 125%, 621%, and 279% in serum concentrations of ceruloplasmin, fibrinogen, haptoglobin and α 1 -acid glycoprotein, respectively, was observed. In sheep the increase in serum concentrations of ceruloplasmin, fibrinogen, haptoglobin and α 1 -acid glycoprotein was of 337%, 90%, 461%, and 225%, respectively. Our results indicate that these APPs have considerable potencial as early and sensible biomarkers of mastitis caused by S. aureus in goats and sheep.

Evaluation of alternatives to cautery disbudding of dairy goat kids using physiological measures of immediate and longer-term pain

“…Normal haptoglobin concentrations for adult goats range between 0.39 and 1.26 g/L (Heller and Johns, 2015;Saidu et al, 2016), which can increase to 1.70 g/L with mastitis (Simplício et al, 2017), which causes inflammation of the mammary tissues and is considered to be painful (Leslie and Petersson-Wolfe, 2012;Fitzpatrick et al, 2013). The values we observed following clove oil injections were substantial, highlighting potentially undesirable side effects.…”

Potential Novel Biomarkers for Mastitis Diagnosis in Sheep

“…Regarding ovine mastitis, to our knowledge, there are only two studies investigating APP response in case of naturally occurring mastitis [28,72], but the studies are not complex, since they investigated the APP concentration only in one diagnostic matrix, Simplicio et al [72] in serum, while Miglio et al [28] in milk. There is also a complex study investigating the protein profile changes in ewes experimentally infected with Mannheimia haemolytica [73], which reveals that after bacterial inoculation the protein profile of blood plasma as well as milk changed significantly.…”

“…For example, in ewes blood plasma there were identified 33 differentially abundant proteins (compared to findings before the challenge): 6 with decrease, 13 with new appearance, and 14 with varying abundance [73] including acute-phase proteins. Simplicio et al [72] investigated serum APP concentration in ewes and goats with naturally acquired staphylococcal mastitis and claimed that increase in serum ceruloplasmin, fibrinogen, haptoglobin, and α1-acid glycoprotein was of 337%, 90%, 461%, and 225%, respectively. While Miglio et al [28] reported the highest milk MAA concentration in ewes with subclinical mastitis comparing to healthy ewes (114.37 ± 41.14 vs. 29.68 ± 27.98 µg/mL).…”

Genetic variation of myostatin MSTN gene and protein polymorphism in three goat populations

“…Overall, nonsynonymous polymorphisms in the coding regions of genes are of major importance since they have a typically significant positive or negative effect on an organism's phenotype (or vice versa) (Grochowska et al, 2020). A polyacrylamide gel electrophoresis is a valuable tool for establishing the quantitative distribution of proteins in goats (Shauket et al, 1998;Simplício et al, 2017) illustrated that the detection and quantification of acute-phase proteins by SDSP-AGE was useful and reliable as a complementary test in goats. The protein profile can be used as a marker depending on the presence or absence bands, band intensity, molecular weight and relative front values (El-Hamamsy et al, 2018).…”