Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Silva, Letícia F.; Pinto, P.S.A.; Ducas, C.T.S.; Santos, Tatiane O.; Nieto, Emilio; Peixoto, Rafaella Paola Meneguete Guimarães

doi:10.1590/1678-4162-7291

Cited by 6 publications

(5 citation statements)

References 18 publications

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“…The ELISA and Immunoblot tests have been applied to determine the epidemiological profile of the TC complex (Santos et al 2013, Silva et al 2015. Records of the occurrence of the TC complex promote streamlined control measures and the use of questionnaires is an important tool for identifying risk factors (Souza et al 2013).…”

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confidence: 99%

Prevalence and risk factors for porcine cysticercosis in rural communities of eastern Minas Gerais, Brazil

et al. 2017

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ABSTRACT.-Acevedo-Nieto E.C., Pinto P.S.A., Silva L.F., Guimarães-Peixoto R.P.M., Santos T.O., Ducas C.T.S. & Bevilacqua P.D. 2017. Prevalence and risk factors for porcine cysticercosis in rural communities of eastern Minas Gerais, Brazil. Pesquisa Veterinária Brasileira 37(9):905-910. Setor de Medicina Veterinária Preventiva, Departamento de Veterinária, Universidade Federal de Viçosa, Avenida PH Rolfs s/n. Viçosa, Cysticercosis is caused by Taenia solium, a parasitic zoonosis that affects human and pigs raised free-range in developing countries. The epidemiology of the taeniosis cysticercosis complex in Brazil is poorly understood especially when it comes to field research. The aim of this study was to estimate the prevalence and identify the risk factors associated with porcine cysticercosis in rural communities located in the east of Minas Gerais (MG), Brazil. From 371 farms in the county of Tumiritinga/MG, 101 farms from 14 communities were randomly sampled. Blood samples from pigs were collected, and epidemiological questionnaires were carried out. The serum samples obtained were analyzed through immunodiagnosis techniques, including ELISA and Western Blot, both for the detection of antibodies. The data obtained by different surveys were analyzed using EpiInfo 3.5.1 software to determine seroprevalence and risk factors associated with cysticercosis. The prevalence of farms with porcine cysticercosis was 9.9% (10/101) and antibody-based seropositive was 5.3% (13/247). The results indicate that cysticercosis occurs in high level in the rural area never studied before. These results suggest the presence of tapeworm carriers contributing to the occurrence and maintenance of this zoonotic life cycle in the county. Regarding risk factors, the most significant determinants for porcine cysticercosis in the field were free-range pig management (OR: 17.4, p: 0.0001), the method of disposal of human faeces in the environmental (OR: 7.6; p 0.012), and the size of the farm. Porcine cysticercosis was diagnosed only in areas represented by Agrarian Reform Settlements. From the results, it is possible to recommend as a means of control and prevention the destination of human faeces in appropriate sanitary landfills and the production of pigs in an enclosed area. Additionally, improving education in the communities sampled will indirectly affect the spreading of this disease. The results draw attention to the largest studies of this zoonotic disease in human settlements from rural areas due to the lack of knowledge about the epidemiology of the taeniosis-cysticercosis complex. The results could serve as the basis to support public policies aimed at creating an effective program to control the taeniosis-cysticercosis complex in the studied area. Awareness of TC among medical doctors, veterinarians, meat animal family producers and the public should be developed through appropriate information and education.

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Prevalence and risk factors for porcine cysticercosis in rural communities of eastern Minas Gerais, Brazil

et al. 2017

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“…Nevertheless the veterinarians and agricultural and livestock technicians of the Secretariat of Agricultural and Fisheries Development of the State of Paraíba (SEDAP) were involved in the fieldwork, so the operational and financial capacity of the SEDAP was taken into consideration and herd sample size was adjusted to their field capacity. For the secondary units, the minimum number of animals to be examined within each herd in order to allow it to be classified as positive was estimated using the concept of aggregate sensitivity and specificity (DOHOO et al, 2003), considering 81.25% (SILVA et al, 2015a) and 98.3% (SILVA et al, 2015b) for the sensitivity and specificity, respectively, for indirect ELISA and immunoblot tests serially applied and 31% for the intra-herd estimated prevalence (ASAAVA et al, 2009). The Herdacc version 3 software (JORDAN, 1995) was used during this process, and the sample size was selected so that the herd sensitivity and specificity values would be ≥ 90%.…”

Section: Samplingmentioning

confidence: 99%

Herd-level spatial cluster analysis of bovine cysticercosis in the state of Paraíba, northeastern Brazil

Maia

Pinto

Peixoto

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of this survey was to identify spatial clustering of bovine cysticercosis-positive herds in the state of Paraíba. The state was divided into three sampling groups: sampling stratum 1 (Sertão mesoregion), sampling stratum 2 (Borborema mesoregion) and sampling stratum 3 (Zona da Mata and Agreste mesoregions), and 2382 cows aging ≥ 24 months from 474 farms were sampled. Serological diagnoses of bovine cysticercosis were initially done by means of indirect ELISA, and positive serum samples were confirmed by a immunoblot test. Herds were deemed positive for cysticercosis if they presented at least one positive animal in herds of up to 29 females, and two positive animals in herds with more than 29 females. The spatial clustering was assessed using the Cuzick-Edwards k-nearest neighbor method and spatial scan statistics. A significant clustering of positive herds was detected in the southern part of the Borborema mesoregion. Given that serological tests for bovine cysticercosis are not widely available, and also that replacement and maintenance of herds through animal purchases is common in the region, it can be concluded that prevention measures should be applied at herd level.

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“…The production and application of recombinant proteins and chimeras have been used in the diagnosis of different diseases such as Toxoplasma gondii [ 14 ] and Leishmania infantum [ 15 ] infection, classical swine fever [ 16 ], and also Taenia solium infection, mainly focusing on human neurocysticercosis [ 17 ] but not on the diagnosis of animal cysticercosis. In the diagnosis of bovine cysticercosis, antigenic proteins of Taenia crassiceps larvae, especially those with low molecular mass, have been used in its immunological diagnosis [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis

et al. 2018

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Bovine cysticercosis is a worldwide distributed zoonosis caused by the larval form of Taenia saginata present in bovine muscles. The diagnosis is based on the postmortem inspection at slaughterhouses and consists of the macroscopic visualization of lesions caused by cysticercosis in muscle sites. However, parasitized animals can pass unnoticed during sanitary inspection. Thus, the objective of this study was to characterize and evaluate the performance of different peptides from different regions of T. saginata for the cysticercosis diagnosis using enzyme-linked immunosorbent assay. We generated and evaluated a new recombinant protein chimera derived from the fusion of different peptides. We selected three distinct regions of T. saginata and predicted six peptides with antigenic potential (EP2–EP7). These peptides were analyzed individually and selected for generating a new chimeric recombinant protein. The new protein was termed rqTSA-25, and its performance rates were: 93.3% sensitivity (confidence interval (CI) = 76–98%), 95.3% specificity (CI = 82–99%), 93% positive predictive value (CI = 76–98%), 95% negative predictive value (CI = 82–99%), and 95% accuracy. In the immunoblot, this protein showed no false positive or false negative reaction. Thus, the use of rqTSA-25 is recommended for the diagnosis of bovine cysticercosis.

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Relevant peptides of Taenia crassiceps for the diagnosis of bovine cysticercosis by immunoblot

Cited by 6 publications

References 18 publications

Prevalence and risk factors for porcine cysticercosis in rural communities of eastern Minas Gerais, Brazil

Prevalence and risk factors for porcine cysticercosis in rural communities of eastern Minas Gerais, Brazil

Herd-level spatial cluster analysis of bovine cysticercosis in the state of Paraíba, northeastern Brazil

Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis

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