Quantification of multi-oocyte follicles in ovaries of bitches

Abstract

The objective of this study was to quantify the number and frequency of monocyte (MnOF) and multi-oocyte (MtOF) follicles in ovaries of bitches subjected to ovary salpingohysterectomy (OSH). Right and left ovaries of 38 bitches were collected after OSH, prepared, and a histological analysis was carried out. The ovaries were subjected to surface and deep histological cuts; the follicles were classified, and the number of follicles and cumulus oophorus complexes (COC) per follicle were quantified for each histological cut. MnOF and MtOF were found in all ovaries, at different developmental stages; primary follicles were grouped in the ovarian cortex, and follicles at other follicular stages presented a random distribution. MtOF containing two, three, four, or more COC were found in the ovaries of bitches, with a decreasing frequency trend, according to the number of COC in the MtOF. The effect of the age, number of estrus, estrus interval, and number of progenies per delivery was not significant for the number and frequency of MtOF in the ovaries of the bitches, whereas the size, number of pregnancies, use and number of contraceptive applications had some effect on the number and frequency of MtOF in the ovaries of the bitches.

Morfometri Oosit Anjing pada Berbagai Umur dan Status Kedewasaan Kelamin

“…Folikulogenesis terjadi 2 -12 minggu setelah lahir dan folikel primordial terlihat pada umur 3 minggu setelah kelahiran (Haenisch-Woehl et al, 2003). Penelitian yang dilakukan oleh Zoppei et al (2021) menunjukan bahwa folikel multi-oosit dapat ditemukan pada ovarium dari berbagai umur dan siklus estrus. Namun sama seperti Lunn dan Wright (2013), oosit sulit dikoleksi pada ovarium anjing prepubertal dimana pada penelitian ini oosit yang berhasil dikoleksi dari kelompok ini paling sedikit adalah 1 oosit.…”