Idade à puberdade e características reprodutivas de novilhas mestiças F1 Holandês x Gir com fenótipos divergentes para consumo alimentar residual

Fonseca, Adolfo Perez; Alves, Bruno Henrique; Campos, M. M.; Costa, Rogério Magnoli; Machado, F. S.; Pereira, L.; Tomich, T. R.; Brandão, Felipe Zandonadi; Borges, Álan Maia

doi:10.1590/1678-4162-11424

Cited by 3 publications

References 18 publications

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Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Fregulia,

Dias,

Campos

et al. 2024

Mol Biol Rep

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Background Methane (CH4) emissions from rumen fermentation are a significant contributor to global warming. Cattle with high CH4 emissions tend to exhibit lower efficiency in milk and meat production, as CH4 production represents a loss of the gross energy ingested by the animal. The objective of this study was to investigate the taxonomic and functional composition of the rumen microbiome associated with methane yield phenotype in dairy cattle raised in tropical areas. Methods and results Twenty-two Girolando (F1 Holstein x Gyr) heifers were classified based on their methane yield (g CH4 / kg dry matter intake (DMI)) as High CH4 yield and Low CH4 yield. Rumen contents were collected and analyzed using amplicon sequencing targeting the 16 and 18S rRNA genes. The diversity indexes showed no differences for the rumen microbiota associated with the high and low methane yield groups. However, the sparse partial least squares discriminant analysis (sPLS-DA) revealed different taxonomic profiles of prokaryotes related to High and Low CH4, but no difference was found for protozoa. The predicted functional profile of both prokaryotes and protozoa differed between High- and Low CH4 groups. Conclusions Our results suggest differences in rumen microbial composition between CH4 yield groups, with specific microorganisms being strongly associated with the Low (e.g. Veillonellaceae_UCG − 001) and High (e.g., Entodinium) CH4 groups. Additionally, specific microbial functions were found to be differentially more abundant in the Low CH4 group, such as K19341, as opposed to the High CH4 group, where K05352 was more prevalent. This study reinforces that identifying the key functional niches within the rumen is vital to understanding the ecological interplay that drives methane production.

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Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Fregulia,

Dias,

Campos

et al. 2024

Mol Biol Rep

View full text Add to dashboard Cite

show abstract

Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Fregulia,

Dias,

Campos

et al. 2023

Preprint

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Background Methane (CH4) emissions from rumen fermentation are a significant contributor to global warming. Cattle with high CH4 emissions tend to exhibit lower efficiency in milk and meat production, as CH4 production represents a loss of the gross energy ingested by the animal. The objective of this study was to investigate the taxonomic and functional composition of the rumen microbiome associated with methane yield phenotype in dairy cattle raised in tropical areas.Methods and Results Twenty-two Girolando (F1 Holstein x Gyr) heifers were classified based on their methane yield (gCH4/kgDMI) as High CH4 yield and Low CH4 yield. Rumen contents were collected and analyzed using amplicon sequencing targeting the 16S and 18S rRNA genes. The diversity indexes showed no differences for the rumen microbiota associated with the methane yield phenotypes. However, the partial least squares discriminant analysis (sPLS-DA) revealed different taxonomic profiles of prokaryotes related to High- and Low CH4, but no difference was found for protozoa. The functional profile of both prokaryotes and protozoa differed between High- and Low CH4 groups.Conclusions Our results suggest differences in rumen microbial composition between CH4 yield groups, with specific microorganisms being strongly associated with the Low- and High CH4 groups (e.g. Veillonellaceae and Entodinium). Additionally, microbial functions were also differentially abundant when comparing the Low- with High CH4 animals (e.g. K19341 and K05352). This study posits that identifying the key functional niches within the rumen is vital to understanding the ecological interplay that drives methane production.

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Taxonomic and predicted functional signatures reveal linkages between the rumen microbiota and feed efficiency in dairy cattle raised in tropical areas

et al. 2022

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Ruminants digest plant biomass more efficiently than monogastric animals due to their symbiotic relationship with a complex microbiota residing in the rumen environment. What remains unclear is the relationship between the rumen microbial taxonomic and functional composition and feed efficiency (FE), especially in crossbred dairy cattle (Holstein x Gyr) raised under tropical conditions. In this study, we selected twenty-two F1 Holstein x Gyr heifers and grouped them according to their residual feed intake (RFI) ranking, high efficiency (HE) (n = 11) and low efficiency (LE) (n = 11), to investigate the effect of FE on the rumen microbial taxa and their functions. Rumen fluids were collected using a stomach tube apparatus and analyzed using amplicon sequencing targeting the 16S (bacteria and archaea) and 18S (protozoa) rRNA genes. Alpha-diversity and beta-diversity analysis revealed no significant difference in the rumen microbiota between the HE and LE animals. Multivariate analysis (sPLS-DA) showed a clear separation of two clusters in bacterial taxonomic profiles related to each FE group, but in archaeal and protozoal profiles, the clusters overlapped. The sPLS-DA also revealed a clear separation in functional profiles for bacteria, archaea, and protozoa between the HE and LE animals. Microbial taxa were differently related to HE (e.g., Howardella and Shuttleworthia) and LE animals (e.g., Eremoplastron and Methanobrevibacter), and predicted functions were significatively different for each FE group (e.g., K03395—signaling and cellular process was strongly related to HE animals, and K13643—genetic information processing was related to LE animals). This study demonstrates that differences in the rumen microbiome relative to FE ranking are not directly observed from diversity indices (Faith’s Phylogenetic Diversity, Pielou’s Evenness, Shannon’s diversity, weighted UniFrac distance, Jaccard index, and Bray–Curtis dissimilarity), but from targeted identification of specific taxa and microbial functions characterizing each FE group. These results shed light on the role of rumen microbial taxonomic and functional profiles in crossbred Holstein × Gyr dairy cattle raised in tropical conditions, creating the possibility of using the microbial signature of the HE group as a biological tool for the development of biomarkers that improve FE in ruminants.

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Idade à puberdade e características reprodutivas de novilhas mestiças F1 Holandês x Gir com fenótipos divergentes para consumo alimentar residual

Cited by 3 publications

References 18 publications

Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Composition of the rumen microbiome and its association with methane yield in dairy cattle raised in tropical conditions

Taxonomic and predicted functional signatures reveal linkages between the rumen microbiota and feed efficiency in dairy cattle raised in tropical areas

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