Colheita farmacológica de sêmen de onças-pardas (Puma concolor: Mammalia: Carnivora: Felidae)

Araújo, Gediendson Ribeiro de; Paula, Tarcízio Antônio Rêgo de; Deco‐Souza, Thyara de; Morato, Ronaldo Gonçalves; Bergo, L.C.F.; Silva, Leanes Cruz da; Jorge‐Neto, Pedro Nacib; Sampaio, Breno Fernandes Barreto

doi:10.1590/1678-4162-11030

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…Currently, pharmacological semen collection by urethral catheterization is the gold-standard method for obtaining semen in wild felines ( Araujo et al, 2018 , 2020b ; Jeong et al, 2018 ; Jorge et al, 2019 ; Kheirkhah et al, 2017 ; Lueders et al, 2012 ), other wild species ( Araujo et al, 2023 ; Curry and Roth, 2016 ; Jeong et al, 2019 ; Silinski et al, 2002 ; Silva et al, 2022 ) and in domestic species ( Cavalero et al, 2019 ; Madrigal-Valverde et al, 2021 ; Silva et al, 2021 ), and it allowed efficient semen recovery from the five male jaguars in this study. The first step in the development of assisted reproduction technology for a species is the effective collection of sperm, which aims to permit, in the future, the growth of genetic variety across in situ and ex situ populations, embracing the concept of One Conservation ( Pizzutto et al, 2021 ).…”

Section: Discussionmentioning

confidence: 82%

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

et al. 2023

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The cryopreservation of jaguar semen must be improved to produce high-quality biobanking doses. Until now, the rare studies of semen freezing in the species have only evaluated glycerol, always with a significant reduction in sperm quality in thawed semen. The purpose of this study was to assess the efficacy of three cryoprotectants, dimethylsulfoxide (DMSO), glycerol (GLY), and methanol (MET), in the cryopreservation of jaguar semen in an LDL-based extender, as well as the effect of thawing temperature on dosage quality. Five mature males with a history of reproduction were used. On the males, an infrared thermal image (IRT) was captured, the spicules and testes were analyzed, and the CASA system was used to evaluate the quality of fresh and thawed sperm. The superficial IRT was 4.6 ± 1.2 °C cooler than the anal sphincter, and the semen measured between 27.3 and 28.7 °C shortly after exiting the urethra. The total motility of fresh sperm was 55.3 ± 22.6%, and progressive motility was 36.3 ± 18%. The total motility of thawed sperm was 5.28 ± 2.51%, 4.49 ± %2.49, and 0.51 ± 0.62% for DMSO, GLY, and MET, respectively. DMSO and GLY performed better than MET, and there was no difference in thawing temperature (37°C 30 s vs. 50°C 12 s). All animals exhibit a considerable level of morphological changes in sperm. Low amounts of total and progressive motility were found in the thawed sperm. Males with a high level of sperm morphological changes were found to be fertile, but the lone male with normospermia was infertile. Thus, we contest the applicability of the commonly used morphological classification for bovines to felid species.

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Section: Discussionmentioning

confidence: 82%

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

et al. 2023

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“…Although electroejaculation is a well‐established semen collection technique in mammals (Silva et al, 2004), the pharmacological semen collection technique has demonstrated higher efficiency in obtaining higher quality semen in several wild species, like wild and domestic felids (Araujo et al 2018, 2020; Araujo, Deco‐Souza, et al, 2020; Jeong et al, 2018; Jorge Neto et al, 2019; Jorge‐Neto et al, 2020; Kheirkhah et al, 2017; Lueders et al, 2012; Silva, Ullony, et al, 2019; Zambelli, Prati, Cunto, Iacono, & Merlo, 2008), bear (Curry & Roth, 2016; Jeong et al, 2019) and even giant anteater (Silva, Araujo, et al, 2019). This study was performed before the description of the pharmacological collection technique in wild animals, being the electroejaculator at the time the best option for semen recovery.…”

Section: Discussionmentioning

confidence: 99%

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

et al. 2021

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The giant anteater (Myrmecophaga tridactyla) is being threatened by natural habitat destruction and fragmentation, illegal hunting and road kills. In this context, the generation of basic information on the reproductive parameters of this species is vital, aiming to improve reproductive management via, amongst others, assisted reproductive technologies. This study aimed to describe the morphological and functional features of semen collected from captive giant anteaters. Electroejaculation was performed in 13 animals housed in zoos located in São Paulo state, Brazil. Semen samples were collected from 13 animals in 16 procedures. Samples were evaluated for volume, motility, vigor, pH, concentration, sperm morphology, and functional tests. The following mean values were obtained: volume 1.28 ± 0.27 mL; motility 28.3 ± 6.2%; vigor 2.4 ± 0.25; concentration 129.4 ± 36.1 × 106 sperm/mL; pH 7.4 ± 0.2. Total acrosome, head, midpiece, and tail sperm abnormalities were 3.2 ± 0.8%, 25.4 ± 3.6%, 20.7 ± 3.2%, and 14.7 ± 2.6%, respectively. Intact acrosome was found in 83.7 ± 3.1% and intact membrane in 81.1 ± 4.0% of all samples collected. Mitochondrial activity was 66.4 ± 6.0% (Class I), 18.7 ± 2.9% (Class II), 8.0 ± 2.0% (Class III), 3.9 ± 1.0% (Class IV), and 3.0 ± 0.9% (Class V). Sperm DNA fragmentation rate was 13.2 ± 3.7%. These results indicated that electroejaculation is a feasible method for semen collection in giant anteaters, allowing a more detailed description of the semen in this species.

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“…Collecting semen through catheterization, as described in detail at the works by Araujo et al (2020) and Rosenfield et al (2020), has become a method of interest, and demanded further training.…”

Section: Discussionmentioning

confidence: 99%

“…The incisors and molars grow continuously, which makes their use as an estimator of age much less feasible (Ojasti, 1973). Besides obtaining radiographic images, additional objectives were to practice orotracheal intubation, which is rather challenging, compared to other species (Girotto, 2018), and urethral catheterization, for future pharmaceutical induced semen collection techniques (Araujo et al, 2020;Rosenfield et al, 2019a). Although several publications have partially described the anatomic and radiographic structures of H. hydrochaeris (Araújo et al, 2012;Bode et al, 2016aBode et al, , 2016bBrombini et al, 2018;Hirota et al, 2018;Kihara, 2017;Schimming et al, 2015;Silva, 2019), this is the first reference on the complete radiographic skeletal anatomy, supported by bone specimens, intended as a reference for scientific studies and clinical-surgical applications.…”

Section: Introductionmentioning

confidence: 99%

Gross Osteology and digital radiography of the common Capybara (Hydrochoerus hydrochaeris), Carl Linnaeus, 1766 for scientific and clinical application

Rosenfield

Paretsis

Yanai

et al. 2020

Braz. J. Vet. Res. Anim. Sci.

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Being able to study anatomical structures of wildlife species for science and clinical practice is of great importance. We aimed to describe the normal gross osteology of the common adult capybara Hydrochoerus hydrochaeris (Linnaeus, 1766), using macerated bone specimens and high-resolution digital radiography. The complete axial and appendicular skeleton was further compared with previously reported anatomical findings. For this purpose, we used three adult capybara cadavers (two females and one male), with a mean bodyweight of ±50 kg. Some H. hydrochaeris-specific morphological characteristics, especially in the skull region, show evidence of adaptation to aquatic life, such as an elongated head shape, with eyes, ears, and nose, located dorsally, to stay above water. Additionally, the entire bone structure demonstrates the size and weight support of the largest rodent and its adaptation with respect to foraging and locomotion behaviors. As a semi-aquatic herbivore, classified as cursorial (having limbs adapted for running), the authors believe it should be reclassified as cursorial-swimming/diving. Moreover, we argue to consider a differentiation between H. hydrochaeris silvestris to H. hydrochaeris synanthrope, due to substantial weight variation, in some cases > 100%. This inevitably has an impact on the development of bone structure, thus influencing habitual adaptation, and consequently, its clinical implications and animal handling. Radiography exams helped in the identification of bone structures, which otherwise were not observed in anatomical specimens, such as ossa sesamoidea, including the ossicle lunulae. Likewise, the radiographs were particularly helpful in overall better comprehension of the intubation and catheterization procedures. We believe this work can contribute as a reference to anatomical studies for students and professionals acting in clinic, surgery, and research.

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Colheita farmacológica de sêmen de onças-pardas (Puma concolor: Mammalia: Carnivora: Felidae)

Cited by 13 publications

References 10 publications

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

Gross Osteology and digital radiography of the common Capybara (Hydrochoerus hydrochaeris), Carl Linnaeus, 1766 for scientific and clinical application

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