Effects of sciatic nerve transection on glucose uptake in the presence and absence of lactate in the frog dorsal root ganglia and spinal cord

Rigon, Fabiana; Horst, Andréa; Kucharski, Luiz Carlos; Silva, R S M; Faccioni-Heuser, Maria Cristina; Partata, Wania Aparecida

doi:10.1590/1519-6984.26012

Cited by 4 publications

(5 citation statements)

References 48 publications

(51 reference statements)

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“…an intact group) was not used because SNT did not induce significant changes in sham-operated frogs (Rigon et al, 2014;Burgess and McIlwain, 1994;Sinicropi et al, 1982). In addition, our AChE activity values showed a similar range as previously described for spinal cord of frog Rana pipiens (Sinicropi et al, 1982).…”

Section: Discussionsupporting

confidence: 76%

“…The SNT was made under anesthesia (prilocaine 0.1 mL/100 g body weight) and sterile conditions as described by Rigon et al (2014Rigon et al ( , 2013. For nerve transection the right sciatic nerve was randomly exposed and completely transected approximately 3 mm distal to the sciatic notch.…”

Section: Surgerymentioning

confidence: 99%

“…This species has been used as a model for the study of SNT-induced neuropathic pain (Rigon et al, 2014(Rigon et al, , 2013Guedes et al, 2004a, b;Partata et al, 2002) because it provides a phylogenetic perspective on the mechanisms of pain research (Rigon et al, 2013). These studies reported alterations that shared similarities with those observed in mammals, while others were unique to this animal species.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

et al. 2017

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Sciatic nerve transection (SNT), a model for studying neuropathic pain, mimics the clinical symptoms of "phantom limb", a pain condition that arises in humans after amputation or transverse spinal lesions. In some vertebrate tissues, this condition decreases acetylcholinesterase (AChE) activity, the enzyme responsible for fast hydrolysis of released acetylcholine in cholinergic synapses. In spinal cord of frog Rana pipiens, this enzyme's activity was not significantly changed in the first days following ventral root transection, another model for studying neuropathic pain. An answerable question is whether SNT decreases AChE activity in spinal cord of frog Lithobates catesbeianus, a species that has been used as a model for studying SNT-induced neuropathic pain. Since each animal model has been created with a specific methodology, and the findings tend to vary widely with slight changes in the method used to induce pain, our study assessed AChE activity 3 and 10 days after complete SNT in lumbosacral spinal cord of adult male bullfrog Lithobates catesbeianus. Because there are time scale differences of motor endplate maturation in rat skeletal muscles, our study also measured the AChE activity in bullfrog tibial posticus (a postural muscle) and gastrocnemius (a typical skeletal muscle that is frequently used to study the motor system) muscles. AChE activity did not show significant changes 3 and 10 days following SNT in spinal cord. Also, no significant change occurred in AChE activity in tibial posticus and gastrocnemius muscles at day 3. However, a significant decrease was found at day 10, with reductions of 18% and 20% in tibial posticus and gastrocnemius, respectively. At present we cannot explain this change in AChE activity. While temporally different, the direction of the change was similar to that described for rats. This similarity indicates that bullfrog is a valid model for investigating AChE activity following SNT.Keywords: acetylcholinesterase, sciatic nerve transection, tibial posticus, gastrocnemius, spinal cord, frog. Efeito da Secção do Nervo Isquiático sobre a atividade da acetilcolinesterase em medula espinal e músculos esqueléticos da rã-touro Lithobates catesbeianus ResumoA transecção do nervo isquiático (SNT), um modelo para estudar dor neuropática, simula os sintomas clínicos do "membro fantasma", uma condição dolorosa que ocorre nos humanos após amputação ou secção completa da medula espinal. Essa condição muda a atividade da acetilcolinesterase (AChE), a enzima responsável pela rápida hidrólise da acetilcolina liberada nas sinapses colinérgicas, em alguns tecidos de vertebrados. Em medula espinal de rã Rana pipiens, a atividade da AChE não foi significativamente alterada nos primeiros dias após a secção da raiz ventral, outro modelo para o estudo da dor neuropática. Uma questão ainda não respondida é se a SNT diminui a atividade da AChE na medula espinal de rã Lithobates catesbeianus, uma espécie que vem sendo usada como modelo em estudos da dor neuropática induzida por SNT. Como...

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Section: Discussionsupporting

confidence: 76%

Section: Surgerymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

et al. 2017

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“…: IR-UU-AEC-3/1656/AD). Animals were anesthetized by intraperitoneal administration of ketamine hydrochloride 5%, 90 mg kg -1 (Ketaset 5%; Alfasan, Woerden, The Netherlands) and xylazine hydrochloride 2%, 5 mg kg -1 (Rompun 2%, Bayer, Leverkusen, Germany) and sterile conditions [27]. Surgery was performed in the control group without nerve transection, but in the other groups, complete left sciatic nerve transection was created.…”

Section: Surgical Proceduresmentioning

confidence: 99%

The regenerative role of mast cells and mesenchymal stem cells in histopathology of sciatic nerve and tibialis cranialis muscle, following denervation in rats

Bakhtiary

Shahrooz

Hobbenaghi

et al. 2022

Preprint

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Atrophy of the leg muscles following sciatic nerve transection can lead to the death of myofibers. The aim of this study was to evaluate the process of nerve and muscle regeneration using mast cells and mesenchymal stem cells. In this study, 90 adult male Wistar rats weighing 150-200g were randomly divided into six main groups (n=15) and each group into three subgroups (2, 4 and 8 weeks, n=5). Groups: control; without surgery, transection (Tr); Fixing the two ends of the transected sciatic nerve (8 mm gap) in silicone tube, scaffold (S); Put collagen gel inside the silicone tube, mast cell (MC); Placed 3×104 mast cells mixed with scaffold, mesenchymal stem cell (MSC); Placed 3×104 mesenchymal stem cells mixed with scaffold, and mast cell-mesenchymal stem cell (MC+MSC); 3×104 of each of the mast cell and mesenchymal stem cells along with scaffold. Animals euthanized and sampled at weeks 2, 4 and 8 for muscle and nerve histological and nerve immunohistochemistical evaluations. Histomorphometric evaluation results of tibialis cranialis muscle (TCM) included: diameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf) and ratio of the blood vessels number to the number of muscle fibers (v/mf) in all treatment groups, especially the MC + MSC group, increased compared to the Tr group but the number of mast cells, the percentage of sarcoplasmolysis and necrosis fibers dramatically decreased. Histomorphometric evaluation results of the nerve in its various parts and immunohistochemistry results also showed improve nerve conduction in all groups, especially the MC + MSC group compared to the Tr group. All results were time dependent and in most cases at 8th week were better the other times. Overall, the results of this study showed that the simultaneous use of MCs and MSCs, especially in the eighth week, accelerated the healing of the sciatic nerve and TCM following reinnervation.

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“…The process of amphibian metamorphosis, characterized by behavioral, morphological, physiological and biochemical changes, is a classic model used to study the transition of vertebrates from an aquatic to a terrestrial environment (Bo et al, 2018;Gao et al, 2018;Kroth et al, 2018;Rigon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic activity of bone markers on Lithobates catesbeianus (Shaw, 1802) growth during the ossification process

et al. 2024

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In order to better understand the ossification processes in anurans our study was carried out on tadpoles and adults of Lithobates catesbeianus. In this sense, we characterized the kinetic properties of alkaline phosphatase with p-nitrophenylphosphatase (pNPP) and pyrophosphate (PPi) and evaluated the activities of tartrate-resistant acid phosphatase and acid phosphatase. The enzyme extracts were obtained from tadpoles and adult femurs, which were divided into epiphysis and diaphysis. After homogenization, the samples were submitted to differential centrifugation to obtain cell membranes and, further, to phospholipase C (PIPLC) treatment, to remove membrane-bound proteins anchored by phosphatidylinositol. The average of specific activity for pNPP hydrolysis (at pH 10.5) by alkaline phosphatase released by phosphatidylinositol-specific phospholipase C (PIPLC) from Bacillus cereus among different bone regions at different animal ages was 1,142.57 U.mg-1, while for PPi hydrolysis (at pH 8.0), it was 1,433.82 U.mg-1. Among the compounds tested for enzymatic activity, the one that influenced the most was EDTA, with approximately 67% of inhibition for pNPPase activity and 77% for PPase activity. In the case of kinetic parameters, the enzyme showed a “Michaelian” behavior for pNPP and PPi hydrolysis. The Km value was around 0.6mM for pNPPase activity and ranged from 0.01 to 0.11mM for PPase activity, indicating that the enzyme has a higher affinity for this substrate. The study of pNPP and PPi hydrolysis by the enzyme revealed that the optimum pH of actuation for pNPP was 10.5, while for PPi, which is considered the true substrate of alkaline phosphatase, was 8.0, close to the physiological value. The results show that regardless of the ossification type that occurs, the same enzyme or isoenzymes act on the different bone regions and different life stages of anurans. The similarity of the results of studies with other vertebrates shows that anurans can be considered excellent animal models for the study of biological calcification.

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Effects of sciatic nerve transection on glucose uptake in the presence and absence of lactate in the frog dorsal root ganglia and spinal cord

Cited by 4 publications

References 48 publications

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

Effect of Sciatic Nerve Transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

The regenerative role of mast cells and mesenchymal stem cells in histopathology of sciatic nerve and tibialis cranialis muscle, following denervation in rats

Enzymatic activity of bone markers on Lithobates catesbeianus (Shaw, 1802) growth during the ossification process

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