Sequencing and multiple antimicrobial resistance of Pseudomonas fluorescens isolated from Nile tilapia fish in Egypt

Abstract

Pseudomonas fluorescens is one of the main causes of septicemic diseases among freshwater fish, causing severe economic losses and decreasing farm efficiency. Thus, this research was aimed to investigate the occurrence of P. fluorescens in Nile Tilapia (O. niloticus) fish in Egypt, gene sequencing of 16SrDNA gene, and antimicrobial susceptibility. P. fluorescens strains were detected in 32% (128\400) of apparently healthy (9%; 36\400) and diseased (23%; 92\400) Nile tilapia fish. The highest prevalence was observed in gills of fish, 31.3% followed by intestine 26.9%, liver 24.2%, and kidneys 17.6%. The PCR results for the 16SrDNA gene of P. fluorescens showed 16SrDNA gene in 30% of examined isolates. Moreover, Homogeny and a strong relationship between strains of P. fluorescens was confirmed using 16SrDNA sequences. Beside the responsibility of 16SrDNA gene on the virulence of P. fluorescens. The results of antimicrobial susceptibility tests revealed that all strains were resistant to piperacillin (100%), followed by ceftazidime (29.7%), and cefepime (25.8%). The strains of P. fluorescence were highly sensitive to cefotaxime (74.2%), followed by ceftriaxone and levofloxacin (70.3% each). Interestingly, 29.7% of strains of P. fluorescens were multiple antimicrobial-resistant (MAR).

Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data

“…A literature search was conducted to analyze which bacterial species have been reported as pathogenic to fish using different terms or keywords such as pathogenic bacteria, fish pathogens, fish pathogenic bacteria, fish bacterial disease, fish diseases, and fish pathogens, among others. This search was performed using PubMed, PubMed Central, ScienceDirect, JSTOR and Scopus databases, resulting in 22 articles relevant to the objective (Almodóvar et al, 2004;Ellermeier and Slauch, 2007;Geng et al, 2007;Swain et al, 2007;Ilardi et al, 2009;Wang et al, 2009;Geng et al,2010a,b;Loch and Faisal, 2010;Troxell et al, 2010;Dang et al, 2011;Johnson et al, 2011;Humbert et al, 2013;Ngwa et al, 2014;Liu et al, 2015;Harke et al, 2016;Menanteau-Ledouble et al, 2016;Bukowska et al, 2017;Pękala-Safińska, 2018;Basri et al, 2020;Li et al, 2020;Shabana et al, 2022). These species were then compared with the taxonomic data obtained herein and were displayed using a relative read abundance on a chord diagram created with the circlize package (Gu et al, 2014).…”

“…P. fluorescens is one of the main causes of septicemic diseases in freshwater fish, causing severe economic losses in aquaculture (Swain et al, 2007;Wang et al, 2009;Shabana et al, 2022). This species is usually associated with pathologies of the skin and fins of fish and has also been reported to cause sudden mortality in fish (Pękala-Safińska, 2018).…”